ENSG00000101003

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262460 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding protein_coding 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 11.1636350 4.11262128 13.1701634 1.52202341 0.7254010 1.676734416 0.51714583 0.53136667 0.42800000 -0.10336667 8.760523e-01 3.581613e-07 FALSE TRUE
ENST00000696793 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding nonsense_mediated_decay 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 2.9130962 0.52808800 4.4537920 0.52808800 0.8561135 3.052355762 0.10147083 0.07063333 0.14403333 0.07340000 4.052994e-01 3.581613e-07 TRUE TRUE
ENST00000696798 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding retained_intron 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 0.3609877 0.37882819 0.3782356 0.37882819 0.3782356 -0.002200357 0.02023333 0.06703333 0.01263333 -0.05440000 9.334349e-01 3.581613e-07   FALSE
ENST00000696804 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding protein_coding 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 2.0138843 0.00000000 3.7549038 0.00000000 1.0108222 8.556469189 0.05871667 0.00000000 0.12000000 0.12000000 3.581613e-07 3.581613e-07 FALSE TRUE
ENST00000696806 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding protein_coding 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 1.0560240 0.41773532 1.5125366 0.02364944 0.2217384 1.831686628 0.05854583 0.05893333 0.04873333 -0.01020000 8.495849e-01 3.581613e-07 FALSE FALSE
ENST00000696870 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding nonsense_mediated_decay 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 1.4202534 0.08806553 2.2069965 0.08806553 1.3098037 4.498716605 0.04153333 0.01560000 0.07170000 0.05610000 7.586594e-01 3.581613e-07   FALSE
ENST00000696875 ENSG00000101003 HEK293_OSMI2_6hA HEK293_TMG_6hB GINS1 protein_coding protein_coding 22.75981 7.237034 30.9182 0.8553481 0.9074422 2.09346 1.0790594 0.70609761 1.0088170 0.17148001 0.1753686 0.508666727 0.07203750 0.10100000 0.03256667 -0.06843333 1.582694e-01 3.581613e-07   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000101003 E001 0.0000000       20 25391008 25391198 191 +      
ENSG00000101003 E002 0.0000000       20 25407611 25407672 62 +      
ENSG00000101003 E003 0.1779838 0.0361273903 1.000000e+00   20 25407673 25407675 3 + 0.070 0.000 -8.942
ENSG00000101003 E004 2.2547742 0.0160267884 6.374182e-01 7.953175e-01 20 25407676 25407682 7 + 0.439 0.529 0.447
ENSG00000101003 E005 3.0408587 0.0083239152 8.497730e-01 9.248452e-01 20 25407683 25407684 2 + 0.559 0.529 -0.135
ENSG00000101003 E006 3.2188426 0.0089691382 7.580672e-01 8.713771e-01 20 25407685 25407685 1 + 0.579 0.529 -0.229
ENSG00000101003 E007 3.6647197 0.0072792407 7.230288e-01 8.499777e-01 20 25407686 25407687 2 + 0.599 0.660 0.269
ENSG00000101003 E008 6.9617730 0.0045679170 7.720764e-01 8.798278e-01 20 25407688 25407697 10 + 0.841 0.804 -0.144
ENSG00000101003 E009 14.3078571 0.0030917772 9.634963e-02 2.753451e-01 20 25407698 25407701 4 + 1.154 0.971 -0.667
ENSG00000101003 E010 18.5352949 0.0037953029 1.890792e-02 9.212258e-02 20 25407702 25407706 5 + 1.266 1.023 -0.871
ENSG00000101003 E011 19.5428947 0.0064163663 2.209919e-02 1.029324e-01 20 25407707 25407708 2 + 1.286 1.047 -0.854
ENSG00000101003 E012 36.4066943 0.0010656106 9.776514e-03 5.715607e-02 20 25407709 25407728 20 + 1.538 1.352 -0.641
ENSG00000101003 E013 37.1081010 0.0010150050 9.927338e-03 5.783556e-02 20 25407729 25407730 2 + 1.546 1.363 -0.631
ENSG00000101003 E014 40.9617633 0.0008979400 2.721211e-02 1.189145e-01 20 25407731 25407738 8 + 1.582 1.435 -0.501
ENSG00000101003 E015 64.9983904 0.0005669247 6.028164e-03 3.986141e-02 20 25407739 25407768 30 + 1.777 1.631 -0.493
ENSG00000101003 E016 110.0575313 0.0005004111 1.333496e-03 1.241071e-02 20 25407769 25407895 127 + 1.998 1.868 -0.438
ENSG00000101003 E017 6.5344006 0.0975570352 5.298708e-01 7.220071e-01 20 25411124 25411286 163 + 0.783 0.886 0.400
ENSG00000101003 E018 3.8585858 0.1925585652 9.251628e-01 9.648380e-01 20 25413457 25413789 333 + 0.631 0.609 -0.097
ENSG00000101003 E019 86.1829883 0.0004971323 1.231830e-05 2.511648e-04 20 25413790 25413854 65 + 1.908 1.698 -0.708
ENSG00000101003 E020 0.9881986 0.0664380259 5.576990e-01   20 25413855 25413933 79 + 0.230 0.342 0.779
ENSG00000101003 E021 61.4677817 0.0006924798 2.031018e-04 2.709674e-03 20 25417104 25417140 37 + 1.762 1.551 -0.717
ENSG00000101003 E022 61.9474492 0.0005984126 1.100366e-03 1.067143e-02 20 25417141 25417202 62 + 1.761 1.580 -0.615
ENSG00000101003 E023 3.5924735 0.0263334381 6.214393e-01 7.848264e-01 20 25417203 25418104 902 + 0.579 0.662 0.363
ENSG00000101003 E024 73.5048104 0.0051413092 4.665630e-02 1.717066e-01 20 25418105 25418195 91 + 1.823 1.704 -0.399
ENSG00000101003 E025 5.5847283 0.0070031049 3.767237e-01 6.096528e-01 20 25419684 25419805 122 + 0.794 0.660 -0.548
ENSG00000101003 E026 0.4764247 0.0215380257 6.569652e-01   20 25420846 25420848 3 + 0.130 0.203 0.776
ENSG00000101003 E027 1.5767104 0.0835889922 2.552424e-01 4.938594e-01 20 25420849 25420988 140 + 0.310 0.530 1.197
ENSG00000101003 E028 8.2104815 0.0720709617 5.880020e-03 3.915164e-02 20 25423505 25425210 1706 + 0.745 1.189 1.664
ENSG00000101003 E029 94.7190353 0.0004316444 6.177400e-03 4.059533e-02 20 25425211 25425327 117 + 1.931 1.812 -0.399
ENSG00000101003 E030 2.1537160 0.0097965390 6.250885e-01 7.873788e-01 20 25427211 25427292 82 + 0.438 0.529 0.450
ENSG00000101003 E031 1.1647096 0.3084532844 3.369792e-01   20 25427293 25427340 48 + 0.230 0.512 1.687
ENSG00000101003 E032 88.3492216 0.0006141954 2.570280e-01 4.958454e-01 20 25441702 25441776 75 + 1.890 1.843 -0.158
ENSG00000101003 E033 0.3807181 0.0309405822 3.714397e-01   20 25443009 25443023 15 + 0.070 0.204 1.776
ENSG00000101003 E034 0.2027342 0.0327390419 1.300583e-01   20 25443024 25443115 92 + 0.000 0.204 12.121
ENSG00000101003 E035 136.7945436 0.0005974880 1.607977e-02 8.209626e-02 20 25445923 25446039 117 + 2.086 2.000 -0.287
ENSG00000101003 E036 560.7210329 0.0002156926 7.084423e-01 8.408098e-01 20 25446040 25447090 1051 + 2.674 2.672 -0.007
ENSG00000101003 E037 298.7261597 0.0028649869 3.655411e-02 1.457213e-01 20 25447091 25447685 595 + 2.383 2.457 0.247
ENSG00000101003 E038 190.6792073 0.0002678957 9.221436e-06 1.956292e-04 20 25447686 25447922 237 + 2.176 2.305 0.433
ENSG00000101003 E039 171.1321537 0.0004146837 2.187440e-06 5.644699e-05 20 25447923 25448102 180 + 2.123 2.271 0.495
ENSG00000101003 E040 81.2833766 0.0005743065 9.216162e-05 1.396179e-03 20 25448103 25448120 18 + 1.794 1.968 0.587
ENSG00000101003 E041 182.9317043 0.0002923100 1.348025e-08 6.137416e-07 20 25448121 25449072 952 + 2.143 2.311 0.559
ENSG00000101003 E042 0.8026527 0.0172848991 2.344320e-01   20 25452442 25452700 259 + 0.272 0.000 -13.344