ENSG00000100997

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000339157 ENSG00000100997 HEK293_OSMI2_6hA HEK293_TMG_6hB ABHD12 protein_coding protein_coding 58.59731 72.96689 36.69232 9.3533 0.4995714 -0.9915685 34.719128 51.562482 14.329954 6.777368 0.6768652 -1.84656106 0.59182500 0.70710000 0.3909667 -0.31613333 1.258497e-04 6.566457e-44 FALSE TRUE
ENST00000491682 ENSG00000100997 HEK293_OSMI2_6hA HEK293_TMG_6hB ABHD12 protein_coding protein_coding 58.59731 72.96689 36.69232 9.3533 0.4995714 -0.9915685 1.785478 0.000000 8.264118 0.000000 0.5572722 9.69246173 0.04462083 0.00000000 0.2255333 0.22553333 6.566457e-44 6.566457e-44 FALSE FALSE
ENST00000672258 ENSG00000100997 HEK293_OSMI2_6hA HEK293_TMG_6hB ABHD12 protein_coding protein_coding 58.59731 72.96689 36.69232 9.3533 0.4995714 -0.9915685 3.465663 4.368913 0.000000 2.248521 0.0000000 -8.77442906 0.04091250 0.05473333 0.0000000 -0.05473333 2.863612e-07 6.566457e-44 FALSE TRUE
ENST00000673524 ENSG00000100997 HEK293_OSMI2_6hA HEK293_TMG_6hB ABHD12 protein_coding nonsense_mediated_decay 58.59731 72.96689 36.69232 9.3533 0.4995714 -0.9915685 8.234453 6.489051 6.425308 2.351378 1.0316157 -0.01421975 0.12820833 0.08740000 0.1744000 0.08700000 3.946401e-01 6.566457e-44 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100997 E001 0.1779838 0.0349044809 1.574429e-01   20 25294742 25294742 1 - 0.191 0.000 -12.720
ENSG00000100997 E002 0.9928126 0.0177857265 6.594916e-01   20 25294743 25294746 4 - 0.323 0.245 -0.543
ENSG00000100997 E003 31.2787229 0.0333535231 4.766795e-03 3.336403e-02 20 25294747 25295030 284 - 1.653 1.348 -1.048
ENSG00000100997 E004 2.0092780 0.0595388700 6.473845e-01 8.017442e-01 20 25300213 25300213 1 - 0.507 0.428 -0.398
ENSG00000100997 E005 6.7374727 0.1629045531 7.499783e-01 8.667667e-01 20 25300214 25300216 3 - 0.889 0.825 -0.245
ENSG00000100997 E006 88.8051904 0.0053725558 6.560000e-01 8.073623e-01 20 25300217 25300254 38 - 1.873 1.905 0.107
ENSG00000100997 E007 230.8662951 0.0054485006 9.371252e-01 9.707651e-01 20 25300255 25300365 111 - 2.301 2.309 0.026
ENSG00000100997 E008 253.7732244 0.0048415362 8.432713e-01 9.212824e-01 20 25300366 25300428 63 - 2.337 2.351 0.049
ENSG00000100997 E009 580.9425765 0.0013571302 2.367509e-05 4.410460e-04 20 25300429 25300698 270 - 2.621 2.731 0.365
ENSG00000100997 E010 208.1647718 0.0018126623 3.224941e-05 5.742303e-04 20 25300699 25300702 4 - 2.138 2.297 0.532
ENSG00000100997 E011 440.8859464 0.0022947658 1.139541e-06 3.181330e-05 20 25300703 25300832 130 - 2.464 2.620 0.521
ENSG00000100997 E012 313.4610754 0.0007404669 5.032716e-03 3.480922e-02 20 25300833 25300884 52 - 2.376 2.458 0.275
ENSG00000100997 E013 11.4557627 0.0124595085 7.565974e-01 8.705813e-01 20 25301249 25301294 46 - 1.060 1.028 -0.116
ENSG00000100997 E014 11.9381407 0.1027041124 9.206191e-01 9.625448e-01 20 25301332 25301378 47 - 1.055 1.055 -0.001
ENSG00000100997 E015 14.2459409 0.0618735123 4.938737e-01 6.966155e-01 20 25301719 25301833 115 - 1.197 1.101 -0.342
ENSG00000100997 E016 21.4893831 0.0487511968 2.912020e-01 5.317848e-01 20 25301834 25302034 201 - 1.383 1.257 -0.440
ENSG00000100997 E017 366.4465226 0.0003387343 3.945429e-01 6.239868e-01 20 25302219 25302276 58 - 2.485 2.511 0.089
ENSG00000100997 E018 354.6007497 0.0001616289 7.826864e-01 8.862156e-01 20 25302277 25302346 70 - 2.481 2.495 0.047
ENSG00000100997 E019 6.3411593 0.0123996169 2.919653e-01 5.326380e-01 20 25303263 25303549 287 - 0.912 0.772 -0.542
ENSG00000100997 E020 263.8632808 0.0004485008 6.109932e-01 7.776123e-01 20 25303550 25303577 28 - 2.368 2.361 -0.021
ENSG00000100997 E021 339.8765397 0.0016447309 5.887323e-01 7.627995e-01 20 25303578 25303628 51 - 2.480 2.469 -0.036
ENSG00000100997 E022 4.9402422 0.0057510851 1.417472e-03 1.302453e-02 20 25306299 25306540 242 - 0.991 0.562 -1.732
ENSG00000100997 E023 372.4653130 0.0009609360 7.739470e-02 2.396288e-01 20 25306833 25306915 83 - 2.539 2.501 -0.127
ENSG00000100997 E024 337.8288345 0.0017888276 3.471546e-01 5.846676e-01 20 25307966 25308045 80 - 2.484 2.463 -0.072
ENSG00000100997 E025 279.7826806 0.0004442613 7.465155e-01 8.646776e-01 20 25308457 25308494 38 - 2.388 2.387 -0.003
ENSG00000100997 E026 311.7349421 0.0007190404 2.008395e-01 4.307666e-01 20 25309446 25309498 53 - 2.452 2.426 -0.086
ENSG00000100997 E027 345.6614558 0.0002074296 8.606992e-03 5.208328e-02 20 25309499 25309575 77 - 2.515 2.466 -0.163
ENSG00000100997 E028 3.6969938 0.0069090873 7.027152e-01 8.372836e-01 20 25310200 25310472 273 - 0.574 0.644 0.307
ENSG00000100997 E029 227.6624165 0.0002240708 6.522293e-05 1.045333e-03 20 25314925 25314970 46 - 2.369 2.273 -0.320
ENSG00000100997 E030 222.9316140 0.0002827731 6.370522e-03 4.155251e-02 20 25317048 25317078 31 - 2.339 2.274 -0.218
ENSG00000100997 E031 3.1011778 0.0078850622 3.387145e-01 5.768637e-01 20 25319052 25319155 104 - 0.686 0.538 -0.649
ENSG00000100997 E032 0.1723744 0.0376664934 1.000000e+00   20 25319773 25319825 53 - 0.000 0.075 9.621
ENSG00000100997 E033 341.6667750 0.0001864332 1.643976e-03 1.462620e-02 20 25320199 25320318 120 - 2.520 2.460 -0.200
ENSG00000100997 E034 343.6287137 0.0002030419 4.469841e-05 7.562865e-04 20 25323325 25323430 106 - 2.535 2.455 -0.266
ENSG00000100997 E035 8.3861530 0.0500027696 6.584910e-01 8.090150e-01 20 25338885 25339004 120 - 0.966 0.920 -0.171
ENSG00000100997 E036 2.0692577 0.1084348675 3.251185e-01 5.643325e-01 20 25339220 25339226 7 - 0.578 0.369 -1.052
ENSG00000100997 E037 254.9539184 0.0003107874 1.209946e-02 6.685431e-02 20 25339227 25339351 125 - 2.388 2.332 -0.187
ENSG00000100997 E038 0.0000000       20 25339622 25339694 73 -      
ENSG00000100997 E039 0.1308682 0.0326491905 1.000000e+00   20 25339695 25339749 55 - 0.000 0.075 9.617
ENSG00000100997 E040 0.1426347 0.0320119168 1.576924e-01   20 25341946 25342096 151 - 0.190 0.000 -12.734
ENSG00000100997 E041 2.4828407 0.0556315891 8.619520e-02 2.567922e-01 20 25389926 25390387 462 - 0.191 0.562 2.264
ENSG00000100997 E042 118.8313443 0.0021980287 1.240964e-06 3.425622e-05 20 25390513 25390839 327 - 1.834 2.069 0.792