ENSG00000100994

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216962 ENSG00000100994 HEK293_OSMI2_6hA HEK293_TMG_6hB PYGB protein_coding protein_coding 47.04446 73.64713 22.33884 11.60191 0.7575362 -1.720625 34.016106 55.209943 19.18456502 8.2867218 0.59690367 -1.524492 0.76456250 0.75186667 0.859200000 0.10733333 0.007057937 0.007057937 FALSE TRUE
ENST00000428458 ENSG00000100994 HEK293_OSMI2_6hA HEK293_TMG_6hB PYGB protein_coding protein_coding 47.04446 73.64713 22.33884 11.60191 0.7575362 -1.720625 4.716560 4.164486 0.04037265 2.0210424 0.04037265 -6.372814 0.06031667 0.05010000 0.001766667 -0.04833333 0.117500858 0.007057937 FALSE TRUE
ENST00000471359 ENSG00000100994 HEK293_OSMI2_6hA HEK293_TMG_6hB PYGB protein_coding processed_transcript 47.04446 73.64713 22.33884 11.60191 0.7575362 -1.720625 4.642988 6.402213 1.50863150 0.4300357 0.15790928 -2.078051 0.09988333 0.09436667 0.067500000 -0.02686667 0.792619289 0.007057937   FALSE
MSTRG.20492.1 ENSG00000100994 HEK293_OSMI2_6hA HEK293_TMG_6hB PYGB protein_coding   47.04446 73.64713 22.33884 11.60191 0.7575362 -1.720625 3.668807 7.870491 1.60526952 2.1107022 0.17211729 -2.286511 0.07523333 0.10363333 0.071566667 -0.03206667 0.563646735 0.007057937 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100994 E001 28.951159 0.0022595963 3.571096e-02 1.434614e-01 20 25248068 25248084 17 + 1.245 1.437 0.669
ENSG00000100994 E002 279.475455 0.0003853222 3.545386e-01 5.913450e-01 20 25248085 25248421 337 + 2.377 2.364 -0.045
ENSG00000100994 E003 213.095515 0.0002946907 9.847418e-03 5.746205e-02 20 25259237 25259338 102 + 2.298 2.234 -0.214
ENSG00000100994 E004 215.022840 0.0002489370 2.790537e-02 1.210064e-01 20 25269129 25269207 79 + 2.293 2.240 -0.174
ENSG00000100994 E005 245.794369 0.0002520093 5.535328e-02 1.921397e-01 20 25271383 25271486 104 + 2.340 2.299 -0.137
ENSG00000100994 E006 326.996917 0.0001970158 1.753873e-01 3.976904e-01 20 25274592 25274723 132 + 2.450 2.429 -0.069
ENSG00000100994 E007 344.879228 0.0002125069 1.733095e-02 8.665678e-02 20 25276646 25276757 112 + 2.488 2.445 -0.146
ENSG00000100994 E008 315.609623 0.0011473068 4.550447e-02 1.688150e-01 20 25277244 25277326 83 + 2.450 2.403 -0.158
ENSG00000100994 E009 393.020325 0.0008046893 3.356076e-03 2.551263e-02 20 25278319 25278462 144 + 2.557 2.495 -0.208
ENSG00000100994 E010 345.505427 0.0006537862 8.330701e-02 2.513761e-01 20 25279057 25279149 93 + 2.481 2.446 -0.116
ENSG00000100994 E011 229.461226 0.0003964426 1.445724e-02 7.608927e-02 20 25280266 25280283 18 + 2.324 2.264 -0.200
ENSG00000100994 E012 379.288757 0.0002086931 4.271041e-01 6.487897e-01 20 25280284 25280412 129 + 2.502 2.495 -0.022
ENSG00000100994 E013 400.804548 0.0008208006 5.000534e-01 7.007862e-01 20 25280949 25281112 164 + 2.499 2.527 0.094
ENSG00000100994 E014 328.534955 0.0004767860 2.126471e-01 4.448433e-01 20 25282033 25282147 115 + 2.452 2.432 -0.067
ENSG00000100994 E015 382.334791 0.0020648799 4.354649e-02 1.639769e-01 20 25283176 25283277 102 + 2.540 2.488 -0.173
ENSG00000100994 E016 420.218800 0.0005038944 1.209413e-01 3.173654e-01 20 25284104 25284251 148 + 2.561 2.536 -0.085
ENSG00000100994 E017 303.565392 0.0015528240 6.965538e-01 8.333489e-01 20 25288425 25288483 59 + 2.384 2.403 0.064
ENSG00000100994 E018 1.493367 0.0676576324 9.590088e-01 9.813964e-01 20 25289848 25289930 83 + 0.348 0.341 -0.048
ENSG00000100994 E019 446.278972 0.0002096493 9.207969e-02 2.675687e-01 20 25290481 25290622 142 + 2.586 2.561 -0.081
ENSG00000100994 E020 585.549548 0.0001222875 5.251553e-02 1.855167e-01 20 25292406 25292613 208 + 2.703 2.680 -0.077
ENSG00000100994 E021 49.924021 0.0761628660 2.999694e-01 5.403695e-01 20 25293901 25294157 257 + 1.723 1.588 -0.457
ENSG00000100994 E022 485.638417 0.0001524254 1.865899e-01 4.125568e-01 20 25294158 25294292 135 + 2.573 2.610 0.124
ENSG00000100994 E023 356.834162 0.0001995166 9.020001e-03 5.391397e-02 20 25295604 25295670 67 + 2.411 2.484 0.244
ENSG00000100994 E024 2469.762793 0.0005645228 1.553149e-14 2.223950e-12 20 25296370 25298305 1936 + 3.225 3.326 0.335