ENSG00000100744

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000438650 ENSG00000100744 HEK293_OSMI2_6hA HEK293_TMG_6hB GSKIP protein_coding protein_coding 9.007604 1.052847 14.46503 0.1930005 0.551724 3.76756 0.3966971 0.1649897 1.147256 0.08287648 0.26991067 2.7253662 0.06138333 0.1583667 0.07863333 -0.07973333 7.740522e-01 6.509455e-06 FALSE TRUE
ENST00000554182 ENSG00000100744 HEK293_OSMI2_6hA HEK293_TMG_6hB GSKIP protein_coding protein_coding 9.007604 1.052847 14.46503 0.1930005 0.551724 3.76756 3.2398136 0.2943236 4.819758 0.11155666 0.32296723 3.9882730 0.32775000 0.3162333 0.33296667 0.01673333 9.466598e-01 6.509455e-06 FALSE TRUE
ENST00000555181 ENSG00000100744 HEK293_OSMI2_6hA HEK293_TMG_6hB GSKIP protein_coding protein_coding 9.007604 1.052847 14.46503 0.1930005 0.551724 3.76756 4.6452975 0.2367158 7.584019 0.23671582 0.54668197 4.9439415 0.42573750 0.1653667 0.52456667 0.35920000 3.229119e-01 6.509455e-06 FALSE TRUE
ENST00000556095 ENSG00000100744 HEK293_OSMI2_6hA HEK293_TMG_6hB GSKIP protein_coding protein_coding 9.007604 1.052847 14.46503 0.1930005 0.551724 3.76756 0.4650404 0.3342244 0.624469 0.05240331 0.07209097 0.8822005 0.14240417 0.3317667 0.04326667 -0.28850000 6.509455e-06 6.509455e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100744 E001 1.4814517 0.0136968632 4.945301e-01 6.971539e-01 14 96363452 96363525 74 + 0.277 0.001 -8.562
ENSG00000100744 E002 19.6849163 0.0018857814 4.916044e-01 6.950599e-01 14 96363526 96363556 31 + 1.096 1.019 -0.282
ENSG00000100744 E003 22.2399347 0.0017485420 2.703577e-01 5.103480e-01 14 96363557 96363568 12 + 1.148 1.019 -0.468
ENSG00000100744 E004 2.0453152 0.1076552964 2.807135e-02 1.214842e-01 14 96363569 96363723 155 + 0.255 0.720 2.408
ENSG00000100744 E005 5.1332015 0.0557148946 1.316669e-05 2.664451e-04 14 96363724 96363925 202 + 0.445 1.170 2.947
ENSG00000100744 E006 19.6117431 0.0047352079 2.487148e-19 6.568817e-17 14 96363926 96365266 1341 + 0.916 1.711 2.800
ENSG00000100744 E007 0.3393995 0.0274424043 1.000000e+00   14 96378938 96379059 122 + 0.079 0.000 -7.993
ENSG00000100744 E008 21.5608983 0.0019243612 9.301704e-02 2.693293e-01 14 96379688 96379701 14 + 1.142 0.922 -0.808
ENSG00000100744 E009 28.3006291 0.0016817350 1.648872e-01 3.838569e-01 14 96379702 96379734 33 + 1.249 1.099 -0.534
ENSG00000100744 E010 32.9314132 0.0080323070 1.014305e-01 2.844138e-01 14 96379735 96379755 21 + 1.312 1.132 -0.636
ENSG00000100744 E011 21.9346721 0.0043441251 8.425407e-02 2.532573e-01 14 96379756 96379788 33 + 1.151 0.921 -0.840
ENSG00000100744 E012 0.9750270 0.0163560596 5.220675e-01   14 96379789 96380292 504 + 0.175 0.312 1.086
ENSG00000100744 E013 94.0098751 0.0009618705 2.924162e-02 1.251014e-01 14 96382247 96382505 259 + 1.747 1.623 -0.423
ENSG00000100744 E014 289.8640606 0.0025560263 4.008307e-01 6.289681e-01 14 96385523 96387288 1766 + 2.220 2.205 -0.049