ENSG00000100664

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216554 ENSG00000100664 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF5 protein_coding protein_coding 108.452 32.08347 187.3155 8.269938 11.93618 2.545196 29.465244 9.894944 42.160248 3.3165813 0.2267722 2.090005 0.32215833 0.29333333 0.22710000 -0.06623333 7.494643e-01 4.988988e-10 FALSE TRUE
ENST00000559249 ENSG00000100664 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF5 protein_coding retained_intron 108.452 32.08347 187.3155 8.269938 11.93618 2.545196 8.135916 1.897182 4.249576 0.5741226 1.0669755 1.159268 0.05686250 0.06256667 0.02350000 -0.03906667 2.260440e-01 4.988988e-10   FALSE
ENST00000561325 ENSG00000100664 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF5 protein_coding protein_coding 108.452 32.08347 187.3155 8.269938 11.93618 2.545196 4.711948 0.000000 18.342692 0.0000000 5.7198626 10.841776 0.02516667 0.00000000 0.09463333 0.09463333 4.988988e-10 4.988988e-10 FALSE FALSE
MSTRG.10223.5 ENSG00000100664 HEK293_OSMI2_6hA HEK293_TMG_6hB EIF5 protein_coding   108.452 32.08347 187.3155 8.269938 11.93618 2.545196 51.591975 17.414558 101.417355 5.2005761 6.1249902 2.541253 0.44777083 0.52570000 0.54170000 0.01600000 9.626426e-01 4.988988e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100664 E001 0.3503582 6.989180e-02 2.881750e-01   14 103333544 103333663 120 + 0.063 0.224 2.121
ENSG00000100664 E002 1.7199652 1.611086e-02 5.152167e-01 0.7118039484 14 103334189 103334191 3 + 0.349 0.480 0.711
ENSG00000100664 E003 8.0907078 3.832290e-03 7.892330e-01 0.8900319803 14 103334192 103334236 45 + 0.875 0.849 -0.100
ENSG00000100664 E004 68.0418512 2.219404e-02 6.252326e-03 0.0409624909 14 103334237 103334260 24 + 1.676 1.932 0.865
ENSG00000100664 E005 6.2850834 1.813095e-02 1.128422e-04 0.0016528556 14 103334261 103334386 126 + 0.611 1.141 2.057
ENSG00000100664 E006 5.3866221 8.315499e-03 3.733480e-04 0.0044716247 14 103334387 103334388 2 + 0.577 1.071 1.955
ENSG00000100664 E007 71.6588542 1.874652e-02 6.685419e-04 0.0071698001 14 103334389 103334391 3 + 1.687 1.975 0.973
ENSG00000100664 E008 404.5766983 3.431344e-03 5.327962e-01 0.7240478139 14 103334392 103334597 206 + 2.497 2.533 0.118
ENSG00000100664 E009 0.8267829 2.012618e-01 3.035210e-01   14 103334803 103334836 34 + 0.166 0.373 1.550
ENSG00000100664 E010 1.1709841 3.262966e-01 2.222561e-01   14 103334861 103334893 33 + 0.214 0.479 1.662
ENSG00000100664 E011 1.1709841 3.262966e-01 2.222561e-01   14 103334894 103334895 2 + 0.214 0.479 1.662
ENSG00000100664 E012 1.9616099 4.168222e-01 8.672716e-02 0.2577485236 14 103334896 103334948 53 + 0.253 0.755 2.572
ENSG00000100664 E013 2.2123875 1.965715e-01 2.152216e-02 0.1010382382 14 103334949 103335038 90 + 0.288 0.797 2.486
ENSG00000100664 E014 1.4223092 1.346810e-01 3.277310e-02 0.1354167029 14 103335039 103335062 24 + 0.209 0.638 2.432
ENSG00000100664 E015 4.2304337 1.134476e-01 1.029532e-01 0.2870624257 14 103335063 103335185 123 + 0.560 0.882 1.330
ENSG00000100664 E016 14.0390267 3.579150e-01 8.309684e-01 0.9143450521 14 103335186 103335480 295 + 1.080 1.087 0.027
ENSG00000100664 E017 13.9999168 5.352428e-01 6.447127e-01 0.7999938758 14 103335481 103335652 172 + 1.074 1.100 0.094
ENSG00000100664 E018 656.8810060 1.126761e-04 1.463081e-02 0.0767060353 14 103335653 103335791 139 + 2.720 2.691 -0.098
ENSG00000100664 E019 323.8870660 1.714168e-04 6.710920e-02 0.2186414496 14 103335792 103335795 4 + 2.415 2.382 -0.108
ENSG00000100664 E020 854.4911842 8.930522e-05 4.800956e-04 0.0054958119 14 103335796 103335932 137 + 2.836 2.796 -0.136
ENSG00000100664 E021 737.1445324 2.276254e-04 2.028560e-04 0.0027070263 14 103336036 103336117 82 + 2.775 2.722 -0.176
ENSG00000100664 E022 4.8744335 6.731534e-03 1.646879e-01 0.3836046411 14 103336118 103336178 61 + 0.628 0.849 0.898
ENSG00000100664 E023 5.4805090 6.987438e-03 5.317251e-01 0.7232476043 14 103336506 103336676 171 + 0.701 0.805 0.421
ENSG00000100664 E024 405.5220943 1.993908e-04 5.110508e-05 0.0008477891 14 103336677 103336684 8 + 2.521 2.440 -0.272
ENSG00000100664 E025 614.8663113 1.334718e-04 8.592112e-06 0.0001839559 14 103336685 103336763 79 + 2.699 2.629 -0.234
ENSG00000100664 E026 630.6992651 1.193352e-04 6.267854e-06 0.0001400192 14 103336764 103336849 86 + 2.709 2.638 -0.235
ENSG00000100664 E027 7.5797821 1.655231e-01 8.418023e-01 0.9204604308 14 103336850 103337115 266 + 0.837 0.849 0.045
ENSG00000100664 E028 596.8983138 2.408001e-04 2.023907e-01 0.4326501649 14 103337116 103337227 112 + 2.677 2.666 -0.036
ENSG00000100664 E029 4.6523211 5.657545e-03 4.496668e-01 0.6654505038 14 103337228 103337737 510 + 0.687 0.567 -0.522
ENSG00000100664 E030 4.1567165 1.756868e-01 4.468983e-01 0.6633708608 14 103338177 103338326 150 + 0.598 0.752 0.650
ENSG00000100664 E031 736.1800896 3.778537e-03 7.528697e-01 0.8684771284 14 103338327 103338472 146 + 2.761 2.777 0.053
ENSG00000100664 E032 871.7589466 2.191302e-03 4.863512e-01 0.6914956259 14 103338735 103338893 159 + 2.833 2.857 0.081
ENSG00000100664 E033 891.3405572 1.013698e-04 2.680562e-02 0.1176890019 14 103339172 103339333 162 + 2.838 2.886 0.158
ENSG00000100664 E034 4.0453155 3.726765e-01 7.714710e-01 0.8794939561 14 103339334 103339638 305 + 0.592 0.717 0.537
ENSG00000100664 E035 1005.0916812 1.068963e-04 6.089031e-03 0.0401665835 14 103339639 103339803 165 + 2.890 2.943 0.176
ENSG00000100664 E036 1.7096037 1.471349e-02 1.064806e-02 0.0608891124 14 103340404 103340426 23 + 0.249 0.702 2.385
ENSG00000100664 E037 999.3711172 9.314016e-05 7.219009e-01 0.8492202118 14 103340427 103340561 135 + 2.894 2.913 0.065
ENSG00000100664 E038 512.7564044 1.513493e-04 8.532219e-01 0.9267636070 14 103340963 103340969 7 + 2.607 2.618 0.036
ENSG00000100664 E039 1039.5987689 1.865795e-03 4.932498e-01 0.6961344376 14 103340970 103341125 156 + 2.907 2.940 0.108
ENSG00000100664 E040 1982.7664696 2.857585e-03 7.840496e-01 0.8869194106 14 103341126 103342158 1033 + 3.191 3.199 0.025
ENSG00000100664 E041 1903.1522228 3.999949e-03 4.379341e-02 0.1645819437 14 103342159 103345025 2867 + 3.162 3.225 0.208