ENSG00000100650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000553369 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding protein_coding 160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 14.587919 5.720776 31.11980 1.5690229 2.0444139 2.44149387 0.08243750 0.04856667 0.18823333 0.13966667 1.447941e-08 9.895981e-32 FALSE FALSE
ENST00000554465 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding retained_intron 160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 10.697253 5.248055 21.48200 1.9320446 0.3306511 2.03119858 0.06340833 0.04353333 0.13010000 0.08656667 5.333704e-04 9.895981e-32 FALSE FALSE
ENST00000556184 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding retained_intron 160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 16.243255 15.168035 16.83031 4.0009614 1.5689240 0.14993376 0.10289583 0.12953333 0.10190000 -0.02763333 6.892675e-01 9.895981e-32 FALSE TRUE
ENST00000556330 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding retained_intron 160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 8.064813 5.853927 16.69688 0.2631351 0.7283645 1.51050311 0.05114583 0.05420000 0.10100000 0.04680000 4.059220e-02 9.895981e-32 FALSE TRUE
ENST00000557154 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding protein_coding 160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 22.560478 14.868727 0.00000 0.4787387 0.0000000 -10.53903541 0.15282083 0.13840000 0.00000000 -0.13840000 9.895981e-32 9.895981e-32 FALSE TRUE
MSTRG.9806.11 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding   160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 11.615273 9.338048 15.62345 3.7514104 2.1337378 0.74189895 0.07587500 0.07726667 0.09443333 0.01716667 8.418664e-01 9.895981e-32 FALSE TRUE
MSTRG.9806.4 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding   160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 25.739262 15.568597 16.21761 2.0998911 1.4000387 0.05888535 0.15831667 0.14320000 0.09833333 -0.04486667 6.155070e-01 9.895981e-32 TRUE TRUE
MSTRG.9806.5 ENSG00000100650 HEK293_OSMI2_6hA HEK293_TMG_6hB SRSF5 protein_coding   160.3875 113.2107 165.1803 18.84729 2.696295 0.5449904 29.543934 26.832959 18.93486 4.4365259 3.5866581 -0.50273735 0.18350417 0.23696667 0.11430000 -0.12266667 2.075485e-02 9.895981e-32 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100650 E001 0.1308682 3.6640514840 8.692293e-01   14 69726900 69727191 292 + 0.001 0.096 7.711
ENSG00000100650 E002 0.4868358 4.0278218194 8.770167e-01   14 69766114 69766467 354 + 0.259 0.095 -1.733
ENSG00000100650 E003 0.5283420 3.9900071952 8.709610e-01   14 69766468 69766728 261 + 0.259 0.110 -1.505
ENSG00000100650 E004 1.3681088 2.2406404071 9.620667e-01   14 69766729 69767092 364 + 0.341 0.386 0.263
ENSG00000100650 E005 2.4994218 0.4569622082 1.000000e+00 1.000000e+00 14 69767093 69767111 19 + 0.394 0.629 1.137
ENSG00000100650 E006 2.8144308 0.3218546730 1.000000e+00 1.000000e+00 14 69767112 69767115 4 + 0.454 0.658 0.945
ENSG00000100650 E007 28.0981273 0.0013290707 3.209624e-01 5.605492e-01 14 69767116 69767141 26 + 1.442 1.490 0.164
ENSG00000100650 E008 65.7157293 0.0010840873 1.384223e-09 7.854090e-08 14 69767142 69767156 15 + 1.980 1.684 -0.998
ENSG00000100650 E009 250.2561303 0.0002872148 4.172549e-01 6.415932e-01 14 69767157 69767171 15 + 2.406 2.405 -0.005
ENSG00000100650 E010 274.9950774 0.0006334631 1.479143e-01 3.593136e-01 14 69767172 69767174 3 + 2.436 2.453 0.057
ENSG00000100650 E011 284.5844134 0.0007291950 8.893347e-02 2.617949e-01 14 69767175 69767181 7 + 2.446 2.471 0.083
ENSG00000100650 E012 302.5163786 0.0008333380 1.735542e-01 3.952643e-01 14 69767182 69767185 4 + 2.477 2.494 0.055
ENSG00000100650 E013 463.0225011 0.0001647049 2.194014e-02 1.023839e-01 14 69767186 69767239 54 + 2.660 2.679 0.064
ENSG00000100650 E014 346.4740737 0.0001591503 6.451927e-02 2.128123e-01 14 69767240 69767251 12 + 2.535 2.551 0.053
ENSG00000100650 E015 228.3221423 0.0002210994 5.912065e-01 7.645249e-01 14 69767252 69767255 4 + 2.369 2.361 -0.026
ENSG00000100650 E016 84.1947191 0.0028093991 9.287260e-01 9.664050e-01 14 69767256 69767380 125 + 1.948 1.920 -0.092
ENSG00000100650 E017 2.9176522 0.0832538214 1.000000e+00 1.000000e+00 14 69767731 69767782 52 + 0.600 0.580 -0.093
ENSG00000100650 E018 2.6104656 0.3385329314 1.000000e+00 1.000000e+00 14 69767783 69767797 15 + 0.601 0.513 -0.404
ENSG00000100650 E019 5.8122896 0.0054974287 3.635683e-01 5.986606e-01 14 69767798 69767902 105 + 0.910 0.777 -0.519
ENSG00000100650 E020 4.6684725 0.0068154268 3.031265e-01 5.437113e-01 14 69767903 69767939 37 + 0.846 0.688 -0.634
ENSG00000100650 E021 5.1085347 0.1332807926 1.000000e+00 1.000000e+00 14 69767940 69767987 48 + 0.762 0.790 0.109
ENSG00000100650 E022 4.5647510 0.7463702489 4.082981e-01 6.346997e-01 14 69767988 69768100 113 + 0.779 0.711 -0.275
ENSG00000100650 E023 5.4236293 0.1121052070 2.970182e-01 5.374626e-01 14 69768101 69768137 37 + 0.902 0.719 -0.721
ENSG00000100650 E024 927.4155732 0.0001332868 4.833077e-01 6.892863e-01 14 69768138 69768282 145 + 2.978 2.967 -0.037
ENSG00000100650 E025 5.1275441 0.0058372571 2.548988e-02 1.137197e-01 14 69768283 69768603 321 + 0.949 0.636 -1.247
ENSG00000100650 E026 419.0974034 0.0009112591 9.363922e-01 9.704268e-01 14 69768604 69768612 9 + 2.637 2.619 -0.058
ENSG00000100650 E027 616.1935565 0.0009947930 1.247537e-01 3.233524e-01 14 69768613 69768674 62 + 2.783 2.798 0.049
ENSG00000100650 E028 6.2987724 0.0069360042 3.826836e-03 2.817745e-02 14 69768675 69768797 123 + 1.047 0.663 -1.493
ENSG00000100650 E029 733.7381307 0.0013655418 6.913459e-03 4.423678e-02 14 69768798 69768896 99 + 2.839 2.884 0.150
ENSG00000100650 E030 83.0405203 0.0006649858 7.653041e-30 5.989228e-27 14 69768897 69769122 226 + 2.147 1.664 -1.628
ENSG00000100650 E031 43.9780497 0.0012695935 1.113902e-15 1.858214e-13 14 69769123 69769181 59 + 1.870 1.404 -1.584
ENSG00000100650 E032 536.1847783 0.0015552644 9.520253e-04 9.520981e-03 14 69769182 69769251 70 + 2.690 2.757 0.220
ENSG00000100650 E033 306.2511747 0.0086405734 3.358107e-01 5.742740e-01 14 69769252 69769510 259 + 2.506 2.470 -0.120
ENSG00000100650 E034 513.7463997 0.0120276474 4.398330e-03 3.136812e-02 14 69769511 69769745 235 + 2.787 2.640 -0.491
ENSG00000100650 E035 667.6550384 0.0150405187 1.458009e-02 7.652711e-02 14 69769746 69770042 297 + 2.892 2.761 -0.437
ENSG00000100650 E036 438.1378765 0.0050151756 8.535259e-02 2.552938e-01 14 69770043 69770447 405 + 2.677 2.613 -0.212
ENSG00000100650 E037 216.2391756 0.0124902004 3.102498e-03 2.403300e-02 14 69770448 69770466 19 + 2.426 2.254 -0.573
ENSG00000100650 E038 580.0572918 0.0016344136 8.487719e-01 9.242236e-01 14 69770467 69770540 74 + 2.773 2.759 -0.044
ENSG00000100650 E039 20.4045787 0.0020127010 4.884469e-09 2.465800e-07 14 69770881 69770994 114 + 1.550 1.072 -1.677
ENSG00000100650 E040 632.3683777 0.0009132321 3.294263e-01 5.683938e-01 14 69770995 69771105 111 + 2.802 2.805 0.010
ENSG00000100650 E041 3.6510345 0.1501111814 1.215299e-01 3.182756e-01 14 69771106 69771108 3 + 0.836 0.476 -1.558
ENSG00000100650 E042 249.8767543 0.0002008652 4.154265e-03 3.001701e-02 14 69771194 69771196 3 + 2.376 2.422 0.153
ENSG00000100650 E043 919.8401013 0.0006436705 1.860046e-09 1.030599e-07 14 69771197 69771544 348 + 2.914 2.999 0.285
ENSG00000100650 E044 307.4066337 0.0012200464 2.250561e-04 2.947914e-03 14 69771545 69771916 372 + 2.438 2.523 0.283
ENSG00000100650 E045 96.6396151 0.0021874832 7.551168e-02 2.358844e-01 14 69771917 69772005 89 + 1.959 2.014 0.186