ENSG00000100532

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216420 ENSG00000100532 HEK293_OSMI2_6hA HEK293_TMG_6hB CGRRF1 protein_coding protein_coding 6.177577 0.9549132 10.42116 0.2045621 0.2647345 3.434356 3.79557203 0.58239440 6.16614239 0.09790807 0.08221656 3.3820761 0.63893750 0.62806667 0.592066667 -0.03600000 0.94678575 0.04723928 FALSE  
ENST00000557317 ENSG00000100532 HEK293_OSMI2_6hA HEK293_TMG_6hB CGRRF1 protein_coding protein_coding 6.177577 0.9549132 10.42116 0.2045621 0.2647345 3.434356 0.85053973 0.20359526 1.67459033 0.12982066 0.24271539 2.9794462 0.13457500 0.19080000 0.161800000 -0.02900000 1.00000000 0.04723928 FALSE  
ENST00000557429 ENSG00000100532 HEK293_OSMI2_6hA HEK293_TMG_6hB CGRRF1 protein_coding retained_intron 6.177577 0.9549132 10.42116 0.2045621 0.2647345 3.434356 0.07980056 0.07427046 0.09525759 0.04610444 0.02741451 0.3208254 0.03336250 0.06786667 0.009266667 -0.05860000 0.04723928 0.04723928 FALSE  
ENST00000557755 ENSG00000100532 HEK293_OSMI2_6hA HEK293_TMG_6hB CGRRF1 protein_coding nonsense_mediated_decay 6.177577 0.9549132 10.42116 0.2045621 0.2647345 3.434356 0.42058767 0.00000000 0.68331238 0.00000000 0.20294382 6.1154336 0.05596667 0.00000000 0.066366667 0.06636667 0.13219869 0.04723928    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100532 E001 3.436622 0.008200999 0.3496470686 0.58695254 14 54509812 54509901 90 + 0.510 0.296 -1.192
ENSG00000100532 E002 2.631756 0.009248451 0.5507851409 0.73688096 14 54509902 54509904 3 + 0.432 0.296 -0.800
ENSG00000100532 E003 2.631756 0.009248451 0.5507851409 0.73688096 14 54509905 54509905 1 + 0.432 0.296 -0.800
ENSG00000100532 E004 2.774391 0.008950892 0.5038492909 0.70348183 14 54509906 54509911 6 + 0.448 0.296 -0.887
ENSG00000100532 E005 34.841228 0.006873949 0.4512697197 0.66653888 14 54509912 54510063 152 + 1.328 1.406 0.271
ENSG00000100532 E006 0.000000       14 54510126 54510184 59 +      
ENSG00000100532 E007 0.000000       14 54510185 54510267 83 +      
ENSG00000100532 E008 49.398216 0.001360590 0.2072355047 0.43850277 14 54522454 54522593 140 + 1.472 1.571 0.338
ENSG00000100532 E009 3.188245 0.007924348 0.9986965481 1.00000000 14 54523150 54523245 96 + 0.465 0.471 0.030
ENSG00000100532 E010 45.310385 0.001032014 0.7598456711 0.87246663 14 54530049 54530226 178 + 1.454 1.438 -0.056
ENSG00000100532 E011 2.408388 0.151184528 0.6808439151 0.82318708 14 54530227 54530405 179 + 0.390 0.469 0.419
ENSG00000100532 E012 2.174660 0.069728537 0.7577449473 0.87125432 14 54530406 54530612 207 + 0.375 0.297 -0.487
ENSG00000100532 E013 2.814166 0.114674151 0.5685909523 0.74923432 14 54530613 54530763 151 + 0.448 0.295 -0.894
ENSG00000100532 E014 31.317332 0.001243937 0.1439191133 0.35329699 14 54530903 54531050 148 + 1.311 1.167 -0.509
ENSG00000100532 E015 0.000000       14 54531051 54531117 67 +      
ENSG00000100532 E016 4.135689 0.118195608 0.0004917476 0.00559869 14 54536073 54537721 1649 + 0.377 1.088 3.026
ENSG00000100532 E017 35.743626 0.001031893 0.2956692182 0.53617066 14 54537722 54537829 108 + 1.360 1.270 -0.317
ENSG00000100532 E018 35.650202 0.001080064 0.2955828313 0.53607829 14 54538063 54538117 55 + 1.360 1.269 -0.317
ENSG00000100532 E019 93.963633 0.000638560 0.8093671755 0.90181454 14 54538118 54539292 1175 + 1.755 1.776 0.070