ENSG00000100380

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216218 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding protein_coding 252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 61.88317 28.81458 82.84945 9.322663 2.871312 1.5233667 0.23839583 0.15540000 0.26596667 0.110566667 2.715647e-01 8.017461e-09 FALSE TRUE
ENST00000411695 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding protein_coding 252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 75.43859 49.97366 99.58911 10.934899 6.820985 0.9946762 0.29854583 0.28773333 0.31856667 0.030833333 7.660127e-01 8.017461e-09 FALSE FALSE
MSTRG.22173.2 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding   252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 18.72567 14.70398 23.65214 4.169683 0.688852 0.6853929 0.07740417 0.08086667 0.07623333 -0.004633333 9.622235e-01 8.017461e-09 FALSE TRUE
MSTRG.22173.3 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding   252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 24.13143 17.82685 32.65280 2.247030 2.433334 0.8727874 0.08950833 0.10660000 0.10450000 -0.002100000 1.000000e+00 8.017461e-09 FALSE TRUE
MSTRG.22173.4 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding   252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 24.50305 27.41799 14.13868 6.957328 1.656344 -0.9549819 0.11479167 0.15303333 0.04500000 -0.108033333 8.017461e-09 8.017461e-09 FALSE TRUE
MSTRG.22173.5 ENSG00000100380 HEK293_OSMI2_6hA HEK293_TMG_6hB ST13 protein_coding   252.247 174.8211 311.9998 37.15025 15.47777 0.8356293 45.36859 34.49015 56.60461 5.890046 2.361191 0.7145719 0.17234167 0.20593333 0.18160000 -0.024333333 8.918847e-01 8.017461e-09 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100380 E001 561.3576897 2.098576e-02 8.194860e-05 1.267003e-03 22 40824535 40825250 716 - 2.841 2.560 -0.937
ENSG00000100380 E002 567.2157658 1.188045e-02 1.387831e-06 3.779436e-05 22 40825251 40825515 265 - 2.843 2.571 -0.905
ENSG00000100380 E003 1131.4153107 8.802229e-03 3.216813e-06 7.880681e-05 22 40825516 40826198 683 - 3.129 2.904 -0.748
ENSG00000100380 E004 2128.0389296 1.544431e-03 9.256212e-02 2.685295e-01 22 40826199 40826659 461 - 3.317 3.332 0.051
ENSG00000100380 E005 776.6850293 1.817509e-03 1.065705e-01 2.932581e-01 22 40826660 40826666 7 - 2.877 2.897 0.068
ENSG00000100380 E006 1003.2867318 6.749289e-04 2.480309e-04 3.197551e-03 22 40827096 40827149 54 - 2.980 3.022 0.141
ENSG00000100380 E007 1144.3850171 8.128950e-05 1.331378e-15 2.186640e-13 22 40827150 40827220 71 - 3.026 3.098 0.238
ENSG00000100380 E008 705.1905866 1.256391e-04 9.735002e-08 3.622321e-06 22 40827221 40827229 9 - 2.823 2.882 0.197
ENSG00000100380 E009 1126.9306194 7.567626e-05 2.885935e-05 5.217141e-04 22 40829626 40829671 46 - 3.038 3.068 0.100
ENSG00000100380 E010 857.6069850 3.160134e-04 7.391629e-01 8.598672e-01 22 40829672 40829674 3 - 2.936 2.924 -0.042
ENSG00000100380 E011 1087.8008095 7.943533e-05 1.225140e-01 3.197827e-01 22 40830840 40830874 35 - 3.035 3.036 0.004
ENSG00000100380 E012 887.9183859 8.206625e-04 5.208140e-01 7.154741e-01 22 40830875 40830878 4 - 2.958 2.926 -0.106
ENSG00000100380 E013 1617.1708125 1.790711e-04 9.367881e-01 9.706254e-01 22 40830879 40830956 78 - 3.213 3.195 -0.059
ENSG00000100380 E014 4.0182212 8.857400e-03 3.656348e-02 1.457438e-01 22 40832087 40832216 130 - 0.807 0.466 -1.490
ENSG00000100380 E015 1620.0549030 2.381304e-04 8.288563e-02 2.505096e-01 22 40832569 40832651 83 - 3.207 3.207 0.000
ENSG00000100380 E016 1031.9921127 9.104335e-05 4.812164e-02 1.752801e-01 22 40832652 40832671 20 - 3.010 3.017 0.022
ENSG00000100380 E017 0.3040503 2.744240e-02 3.445401e-01   22 40835106 40835176 71 - 0.188 0.000 -10.683
ENSG00000100380 E018 0.3503582 4.967280e-02 7.699937e-01   22 40835393 40835559 167 - 0.104 0.141 0.505
ENSG00000100380 E019 1899.5536164 5.433121e-05 5.187788e-02 1.841644e-01 22 40835560 40835670 111 - 3.277 3.277 0.001
ENSG00000100380 E020 1.0295171 3.659895e-02 6.252345e-01   22 40835671 40835802 132 - 0.258 0.334 0.512
ENSG00000100380 E021 1464.2075015 2.976382e-04 5.365135e-01 7.266896e-01 22 40835803 40835887 85 - 3.168 3.158 -0.033
ENSG00000100380 E022 1.7278896 1.120751e-01 6.117540e-01 7.780441e-01 22 40835888 40835949 62 - 0.371 0.472 0.541
ENSG00000100380 E023 1374.9574451 9.206100e-05 2.875735e-01 5.283269e-01 22 40840626 40840692 67 - 3.140 3.134 -0.020
ENSG00000100380 E024 1342.2173074 5.469608e-04 5.061858e-01 7.050862e-01 22 40844839 40844909 71 - 3.138 3.107 -0.104
ENSG00000100380 E025 6.4647096 4.850841e-03 3.445855e-02 1.401172e-01 22 40847970 40848044 75 - 0.748 0.993 0.941
ENSG00000100380 E026 1445.0581975 6.655956e-04 3.987789e-02 1.543975e-01 22 40848294 40848369 76 - 3.179 3.125 -0.178
ENSG00000100380 E027 2.2482370 1.285636e-01 6.322281e-01 7.918231e-01 22 40850173 40850230 58 - 0.540 0.411 -0.649
ENSG00000100380 E028 1281.8368738 9.363465e-04 6.195962e-01 7.835349e-01 22 40850823 40850880 58 - 3.111 3.097 -0.047
ENSG00000100380 E029 0.1426347 3.270815e-02 7.275928e-01   22 40854538 40854598 61 - 0.104 0.000 -9.682
ENSG00000100380 E030 1685.6093374 1.372832e-03 2.918158e-01 5.324935e-01 22 40856431 40856964 534 - 3.225 3.220 -0.017