ENSG00000100354

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335727 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding protein_coding 3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 1.5966731 2.1501481 1.8847455 0.2536044 0.07194900 -0.1891262 0.41827500 0.50960000 0.34760000 -0.16200000 0.09348400 0.01323848 FALSE TRUE
ENST00000402203 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding protein_coding 3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 0.4234750 0.5506738 0.3152338 0.1648662 0.04503868 -0.7856845 0.11791667 0.12453333 0.05903333 -0.06550000 0.42305456 0.01323848 FALSE TRUE
ENST00000446273 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding protein_coding 3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 0.9456411 0.6592385 1.7804685 0.1221594 0.02803179 1.4197448 0.22602500 0.15343333 0.33006667 0.17663333 0.01323848 0.01323848 FALSE TRUE
ENST00000489500 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding retained_intron 3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 0.1778190 0.0000000 0.5923561 0.0000000 0.30531603 5.9125448 0.03540000 0.00000000 0.10140000 0.10140000 0.29123262 0.01323848   FALSE
MSTRG.22186.1 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding   3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 0.3312452 0.1308498 0.8414369 0.1308498 0.10947480 2.5957421 0.08310833 0.04066667 0.15326667 0.11260000 0.30541406 0.01323848 FALSE TRUE
MSTRG.22186.4 ENSG00000100354 HEK293_OSMI2_6hA HEK293_TMG_6hB TNRC6B protein_coding   3.900031 4.237288 5.454984 0.5225273 0.3798015 0.3636751 0.1649677 0.4398547 0.0000000 0.2448517 0.00000000 -5.4913873 0.04823750 0.09173333 0.00000000 -0.09173333 0.24783794 0.01323848 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100354 E001 1.3629473 0.3739958126 2.518035e-01   22 40044817 40044840 24 + 0.494 0.209 -1.779
ENSG00000100354 E002 4.6316505 0.0058446326 6.118926e-02 2.054437e-01 22 40044841 40044998 158 + 0.856 0.606 -1.024
ENSG00000100354 E003 0.1308682 0.0308792895 5.561257e-01   22 40045474 40045588 115 + 0.000 0.115 10.820
ENSG00000100354 E004 7.8260872 0.0040761456 5.499265e-02 1.913262e-01 22 40117055 40117128 74 + 1.039 0.830 -0.786
ENSG00000100354 E005 5.7369827 0.0130958894 2.201263e-01 4.536712e-01 22 40125772 40125773 2 + 0.896 0.745 -0.592
ENSG00000100354 E006 9.6261504 0.0035083092 1.461710e-01 3.566601e-01 22 40125774 40125862 89 + 1.091 0.949 -0.520
ENSG00000100354 E007 7.9489447 0.0037758413 4.078643e-02 1.567564e-01 22 40156115 40156182 68 + 1.053 0.831 -0.834
ENSG00000100354 E008 2.7079761 0.0089398136 1.362313e-01 3.417274e-01 22 40177925 40177941 17 + 0.678 0.451 -1.049
ENSG00000100354 E009 3.5885841 0.0148882890 3.519280e-01 5.889848e-01 22 40177942 40177948 7 + 0.736 0.605 -0.553
ENSG00000100354 E010 20.2998927 0.0020039247 6.270158e-02 2.088204e-01 22 40177949 40178140 192 + 1.398 1.269 -0.449
ENSG00000100354 E011 23.0553393 0.0015087881 2.859648e-02 1.231355e-01 22 40246015 40246102 88 + 1.454 1.310 -0.500
ENSG00000100354 E012 0.0000000       22 40246103 40246362 260 +      
ENSG00000100354 E013 11.4890689 0.0030927145 1.923896e-02 9.329597e-02 22 40251179 40251200 22 + 1.197 0.978 -0.795
ENSG00000100354 E014 36.0891332 0.0030315598 1.517741e-01 3.648849e-01 22 40261832 40262173 342 + 1.603 1.526 -0.263
ENSG00000100354 E015 23.8394779 0.0105490532 2.213927e-01 4.551488e-01 22 40264688 40264797 110 + 1.442 1.348 -0.324
ENSG00000100354 E016 48.1450393 0.0024638331 1.328048e-02 7.156731e-02 22 40264798 40265002 205 + 1.750 1.627 -0.418
ENSG00000100354 E017 237.1643727 0.0006951688 6.758674e-14 8.695290e-12 22 40265003 40266800 1798 + 2.456 2.287 -0.564
ENSG00000100354 E018 42.0408058 0.0009299074 1.491468e-01 3.610168e-01 22 40266801 40267036 236 + 1.668 1.603 -0.221
ENSG00000100354 E019 13.2607934 0.0023271158 8.709770e-02 2.584517e-01 22 40270122 40270280 159 + 1.053 1.225 0.617
ENSG00000100354 E020 45.7356121 0.0048492425 3.852385e-01 6.166801e-01 22 40273425 40273600 176 + 1.692 1.655 -0.128
ENSG00000100354 E021 0.0000000       22 40276840 40276981 142 +      
ENSG00000100354 E022 31.9718698 0.0037202104 4.222133e-01 6.453086e-01 22 40277077 40277151 75 + 1.543 1.504 -0.135
ENSG00000100354 E023 25.5917269 0.0013970066 7.539858e-01 8.691151e-01 22 40277999 40278044 46 + 1.427 1.418 -0.030
ENSG00000100354 E024 39.4577928 0.0017490115 4.670244e-01 6.780534e-01 22 40279995 40280143 149 + 1.576 1.632 0.189
ENSG00000100354 E025 0.2027342 0.0337785459 5.544242e-01   22 40281119 40281289 171 + 0.000 0.115 10.800
ENSG00000100354 E026 35.8749412 0.0012432415 5.468625e-01 7.340735e-01 22 40285645 40285770 126 + 1.539 1.587 0.163
ENSG00000100354 E027 34.2243868 0.0052521370 2.599448e-01 4.989669e-01 22 40300455 40300586 132 + 1.499 1.586 0.300
ENSG00000100354 E028 0.2735028 0.0261041505 9.106239e-01   22 40300856 40300909 54 + 0.128 0.115 -0.178
ENSG00000100354 E029 23.9525291 0.0106188765 8.544540e-01 9.272840e-01 22 40300910 40301005 96 + 1.386 1.419 0.113
ENSG00000100354 E030 38.9302261 0.0014356554 7.713987e-01 8.794509e-01 22 40301150 40301333 184 + 1.607 1.603 -0.012
ENSG00000100354 E031 0.2027342 0.0337785459 5.544242e-01   22 40301334 40301495 162 + 0.000 0.115 10.800
ENSG00000100354 E032 42.3201585 0.0018278460 6.139044e-01 7.794773e-01 22 40308512 40308649 138 + 1.642 1.626 -0.052
ENSG00000100354 E033 46.6882110 0.0029649449 9.765308e-01 9.900208e-01 22 40310817 40310993 177 + 1.668 1.682 0.048
ENSG00000100354 E034 42.6315013 0.0009489252 9.339399e-01 9.690931e-01 22 40312505 40312651 147 + 1.625 1.642 0.057
ENSG00000100354 E035 37.1202952 0.0015191372 5.417486e-01 7.303356e-01 22 40312902 40312997 96 + 1.548 1.597 0.168
ENSG00000100354 E036 64.4864270 0.0007175086 6.644968e-02 2.170716e-01 22 40315283 40315507 225 + 1.761 1.855 0.320
ENSG00000100354 E037 38.8369147 0.0013823496 4.547820e-01 6.693267e-01 22 40315942 40316012 71 + 1.565 1.620 0.189
ENSG00000100354 E038 45.8739755 0.0009214296 9.325948e-01 9.684583e-01 22 40321090 40321229 140 + 1.665 1.673 0.027
ENSG00000100354 E039 1.9656107 0.0317850068 8.267019e-01 9.118856e-01 22 40321230 40321799 570 + 0.485 0.452 -0.166
ENSG00000100354 E040 39.0339311 0.0015738580 3.470942e-01 5.846169e-01 22 40322854 40323038 185 + 1.565 1.632 0.229
ENSG00000100354 E041 40.9825332 0.0010392128 2.078730e-01 4.392497e-01 22 40323039 40323348 310 + 1.576 1.659 0.281
ENSG00000100354 E042 11.3324595 0.0039887821 7.970712e-01 8.945881e-01 22 40323349 40323375 27 + 1.091 1.077 -0.049
ENSG00000100354 E043 848.3571436 0.0009647076 3.216138e-09 1.681571e-07 22 40323376 40335808 12433 + 2.876 2.966 0.300