ENSG00000100299

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216124 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding protein_coding 14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 2.727643 3.9917609 1.8165462 0.8597950 0.2256457 -1.1315167 0.18901250 0.19970000 0.2207667 0.02106667 9.334822e-01 1.450497e-07 FALSE TRUE
ENST00000356098 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding protein_coding 14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 3.531554 5.2355207 1.4848769 1.2606436 0.7882915 -1.8110593 0.24233750 0.25843333 0.1739333 -0.08450000 8.498515e-01 1.450497e-07 FALSE TRUE
ENST00000395621 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding protein_coding 14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 0.964639 0.5196684 0.8685429 0.2629264 0.8685429 0.7300233 0.07328750 0.02736667 0.1144000 0.08703333 8.673302e-01 1.450497e-07 FALSE TRUE
ENST00000453344 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding protein_coding 14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 1.457034 2.2986368 0.0000000 0.4567129 0.0000000 -7.8508974 0.09032083 0.12010000 0.0000000 -0.12010000 1.450497e-07 1.450497e-07 FALSE TRUE
ENST00000551731 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding retained_intron 14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 3.912683 5.9561353 2.6132312 0.8743442 0.3005699 -1.1854517 0.27451250 0.29666667 0.3171333 0.02046667 9.485123e-01 1.450497e-07 FALSE TRUE
MSTRG.22380.7 ENSG00000100299 HEK293_OSMI2_6hA HEK293_TMG_6hB ARSA protein_coding   14.40435 19.90949 8.189643 1.504415 0.3408286 -1.280547 1.328209 1.4510375 1.1755697 0.2857541 0.1836197 -0.3014127 0.09625417 0.07146667 0.1459333 0.07446667 2.651828e-01 1.450497e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100299 E001 0.1426347 6.0059485027 0.337215548   22 50620663 50620720 58 - 0.209 0.000 -11.215
ENSG00000100299 E002 7.8766979 0.0956053589 0.596071369 0.76777815 22 50622754 50622872 119 - 0.914 0.848 -0.253
ENSG00000100299 E003 15.6734883 0.0232873575 1.000000000 1.00000000 22 50622873 50623027 155 - 1.110 1.127 0.062
ENSG00000100299 E004 52.9644252 0.0440688016 0.924668371 0.96467006 22 50623028 50623773 746 - 1.609 1.633 0.082
ENSG00000100299 E005 16.2518760 0.0296161919 0.515124276 0.71172718 22 50623774 50623895 122 - 1.039 1.160 0.435
ENSG00000100299 E006 32.6406502 0.0427490923 0.377717202 0.61032996 22 50623896 50624169 274 - 1.494 1.408 -0.298
ENSG00000100299 E007 97.8941805 0.0151662220 0.077136393 0.23907312 22 50624170 50625017 848 - 1.986 1.867 -0.400
ENSG00000100299 E008 25.6520874 0.0101916697 0.003195585 0.02458030 22 50625018 50625027 10 - 1.531 1.265 -0.923
ENSG00000100299 E009 22.4897622 0.0146376184 0.016522085 0.08371635 22 50625028 50625028 1 - 1.457 1.221 -0.824
ENSG00000100299 E010 23.2582831 0.0089823957 0.007245799 0.04579810 22 50625029 50625032 4 - 1.478 1.233 -0.853
ENSG00000100299 E011 128.3978255 0.0013546723 0.126633814 0.32647155 22 50625033 50625362 330 - 2.068 1.996 -0.242
ENSG00000100299 E012 85.3853139 0.0018665996 1.000000000 1.00000000 22 50625363 50625464 102 - 1.845 1.833 -0.040
ENSG00000100299 E013 96.9701784 0.0004674147 0.305558743 0.54621007 22 50625579 50625681 103 - 1.862 1.899 0.125
ENSG00000100299 E014 102.5225812 0.0005182043 0.138700567 0.34554685 22 50625936 50626063 128 - 1.870 1.927 0.190
ENSG00000100299 E015 103.8264956 0.0162503818 0.952593009 0.97829720 22 50626154 50626278 125 - 1.919 1.917 -0.007
ENSG00000100299 E016 117.7096052 0.0038115764 0.289749975 0.53030791 22 50626591 50626760 170 - 2.023 1.962 -0.205
ENSG00000100299 E017 23.9337657 0.0953065951 0.220770078 0.45436390 22 50626761 50626833 73 - 1.409 1.266 -0.498
ENSG00000100299 E018 100.1166234 0.0004286053 0.882069627 0.94240157 22 50626834 50626989 156 - 1.907 1.905 -0.009
ENSG00000100299 E019 56.5105624 0.0039584914 0.071456173 0.22771015 22 50626990 50627052 63 - 1.566 1.684 0.404
ENSG00000100299 E020 21.5441402 0.0565340960 0.597878832 0.76887340 22 50627053 50627165 113 - 1.310 1.240 -0.246
ENSG00000100299 E021 120.8941242 0.0013015219 0.027855605 0.12087085 22 50627166 50627406 241 - 1.914 2.001 0.295
ENSG00000100299 E022 124.1219149 0.0010072987 0.423194821 0.64601142 22 50627556 50627792 237 - 1.979 2.003 0.082
ENSG00000100299 E023 77.2511057 0.0017118579 0.340962507 0.57890173 22 50627793 50627897 105 - 1.759 1.800 0.140
ENSG00000100299 E024 52.2794413 0.0060002616 0.147772890 0.35910872 22 50627898 50627945 48 - 1.542 1.639 0.333
ENSG00000100299 E025 64.3825663 0.0066773662 0.185630367 0.41138300 22 50627946 50627990 45 - 1.644 1.727 0.281
ENSG00000100299 E026 80.6222863 0.0044825766 0.218162190 0.45122226 22 50627991 50628173 183 - 1.758 1.820 0.212