ENSG00000100294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000290429 ENSG00000100294 HEK293_OSMI2_6hA HEK293_TMG_6hB MCAT protein_coding protein_coding 66.53894 100.454 26.95942 21.73684 0.3805886 -1.897282 12.83010 17.13156 8.335259 3.717125 0.5698787 -1.038469 0.2120250 0.1711667 0.3088333 0.1376667 0.001202232 0.001202232 FALSE TRUE
ENST00000327555 ENSG00000100294 HEK293_OSMI2_6hA HEK293_TMG_6hB MCAT protein_coding protein_coding 66.53894 100.454 26.95942 21.73684 0.3805886 -1.897282 34.25930 51.58428 11.764355 11.207452 0.6657690 -2.131563 0.5026833 0.5154000 0.4363000 -0.0791000 0.470015527 0.001202232 FALSE TRUE
MSTRG.22277.3 ENSG00000100294 HEK293_OSMI2_6hA HEK293_TMG_6hB MCAT protein_coding   66.53894 100.454 26.95942 21.73684 0.3805886 -1.897282 17.98621 29.76003 5.902840 7.093242 0.2827931 -2.331937 0.2586750 0.2922000 0.2192000 -0.0730000 0.221551216 0.001202232 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100294 E001 101.369130 0.253302338 0.90862311 0.95644235 22 43132209 43132879 671 - 1.949 1.902 -0.159
ENSG00000100294 E002 238.981793 0.450237144 0.43496106 0.65494887 22 43132880 43132994 115 - 2.182 2.301 0.400
ENSG00000100294 E003 1092.457722 1.350270612 0.50111444 0.70158590 22 43132995 43133486 492 - 2.798 2.967 0.563
ENSG00000100294 E004 477.248514 0.974406551 0.50749661 0.70602602 22 43137081 43137298 218 - 2.473 2.602 0.428
ENSG00000100294 E005 13.329313 0.153180200 0.01227856 0.06756127 22 43140830 43141055 226 - 1.309 0.968 -1.228
ENSG00000100294 E006 13.327926 0.213234838 0.06376104 0.21116033 22 43141056 43141161 106 - 1.268 0.997 -0.972
ENSG00000100294 E007 406.808493 0.030568742 0.15555009 0.37023318 22 43141162 43141249 88 - 2.467 2.521 0.181
ENSG00000100294 E008 7.488584 0.004649838 0.57545756 0.75396501 22 43142892 43142925 34 - 0.834 0.852 0.071
ENSG00000100294 E009 644.521555 0.284474696 0.68297563 0.82455287 22 43142926 43143398 473 - 2.588 2.735 0.487