ENSG00000100281

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216106 ENSG00000100281 HEK293_OSMI2_6hA HEK293_TMG_6hB HMGXB4 protein_coding protein_coding 19.36429 7.958045 32.73955 1.787226 0.9922577 2.039177 7.139143 3.4925073 11.474749 0.6603270 1.3917993 1.7132593 0.38979583 0.45246667 0.3492667 -0.1032000 5.620736e-01 1.300865e-09 FALSE TRUE
ENST00000418170 ENSG00000100281 HEK293_OSMI2_6hA HEK293_TMG_6hB HMGXB4 protein_coding nonsense_mediated_decay 19.36429 7.958045 32.73955 1.787226 0.9922577 2.039177 2.426927 0.0000000 4.645359 0.0000000 1.1932541 8.8627485 0.08793333 0.00000000 0.1418333 0.1418333 1.349571e-08 1.300865e-09 FALSE TRUE
ENST00000455359 ENSG00000100281 HEK293_OSMI2_6hA HEK293_TMG_6hB HMGXB4 protein_coding protein_coding 19.36429 7.958045 32.73955 1.787226 0.9922577 2.039177 2.568195 0.0000000 4.856705 0.0000000 1.1066335 8.9268014 0.08380417 0.00000000 0.1474000 0.1474000 1.300865e-09 1.300865e-09 FALSE TRUE
MSTRG.22022.1 ENSG00000100281 HEK293_OSMI2_6hA HEK293_TMG_6hB HMGXB4 protein_coding   19.36429 7.958045 32.73955 1.787226 0.9922577 2.039177 3.518125 0.3914833 5.638839 0.3914833 0.9715121 3.8145423 0.16189167 0.04163333 0.1719667 0.1303333 2.665347e-01 1.300865e-09 FALSE TRUE
MSTRG.22022.2 ENSG00000100281 HEK293_OSMI2_6hA HEK293_TMG_6hB HMGXB4 protein_coding   19.36429 7.958045 32.73955 1.787226 0.9922577 2.039177 2.733565 3.4800414 4.343693 1.1241024 0.8185313 0.3189956 0.21576250 0.41123333 0.1343000 -0.2769333 7.446950e-03 1.300865e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100281 E001 0.1426347 0.0330508880 1.000000e+00   22 35257430 35257451 22 + 0.071 0.001 -6.583
ENSG00000100281 E002 1.7727401 0.0287686846 3.727997e-01 6.063077e-01 22 35257452 35257486 35 + 0.353 0.522 0.893
ENSG00000100281 E003 1.7727401 0.0287686846 3.727997e-01 6.063077e-01 22 35257487 35257488 2 + 0.353 0.522 0.893
ENSG00000100281 E004 1.9036082 0.0526448479 2.252825e-01 4.596981e-01 22 35257489 35257492 4 + 0.353 0.590 1.203
ENSG00000100281 E005 3.3684917 0.0314358964 9.869629e-01 9.953147e-01 22 35257493 35257495 3 + 0.587 0.591 0.017
ENSG00000100281 E006 64.9493113 0.0221697878 6.323825e-02 2.099886e-01 22 35257496 35257557 62 + 1.782 1.642 -0.475
ENSG00000100281 E007 4.2370393 0.1492413298 4.132940e-01 6.385845e-01 22 35258083 35258588 506 + 0.626 0.785 0.661
ENSG00000100281 E008 4.2769816 0.2439011890 3.209469e-01 5.605406e-01 22 35261854 35262322 469 + 0.585 0.848 1.088
ENSG00000100281 E009 48.4570722 0.0007997554 6.938996e-03 4.434748e-02 22 35262323 35262421 99 + 1.664 1.496 -0.572
ENSG00000100281 E010 0.9832093 0.0208555734 5.806837e-01   22 35262763 35263077 315 + 0.234 0.336 0.716
ENSG00000100281 E011 140.8318325 0.0007423666 1.185932e-05 2.427366e-04 22 35263078 35263226 149 + 2.118 1.952 -0.555
ENSG00000100281 E012 113.8196847 0.0004820283 7.435186e-04 7.808210e-03 22 35263796 35263874 79 + 2.020 1.882 -0.461
ENSG00000100281 E013 58.2943022 0.0006927651 3.445235e-01 5.820998e-01 22 35263982 35264096 115 + 1.717 1.668 -0.169
ENSG00000100281 E014 425.3226003 0.0002043601 2.159981e-02 1.012880e-01 22 35264648 35265603 956 + 2.571 2.528 -0.143
ENSG00000100281 E015 75.7710283 0.0006365902 2.391706e-01 4.756384e-01 22 35283962 35284043 82 + 1.802 1.858 0.190
ENSG00000100281 E016 87.9883248 0.0004841299 7.491607e-01 8.662631e-01 22 35285997 35286061 65 + 1.882 1.869 -0.044
ENSG00000100281 E017 4.6979386 0.2119675718 9.876310e-01 9.956620e-01 22 35286882 35287346 465 + 0.702 0.659 -0.181
ENSG00000100281 E018 114.1267649 0.0004941617 3.484845e-01 5.859378e-01 22 35287347 35287452 106 + 2.004 1.969 -0.117
ENSG00000100281 E019 167.4831009 0.0016535935 8.887234e-01 9.460230e-01 22 35288238 35288407 170 + 2.158 2.165 0.022
ENSG00000100281 E020 169.4975142 0.0005981606 5.243515e-02 1.853253e-01 22 35292992 35293114 123 + 2.177 2.114 -0.212
ENSG00000100281 E021 927.7315914 0.0001846484 7.342207e-14 9.396199e-12 22 35293607 35295807 2201 + 2.877 2.960 0.276