ENSG00000100234

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266085 ENSG00000100234 HEK293_OSMI2_6hA HEK293_TMG_6hB TIMP3 protein_coding protein_coding 12.44853 12.55171 9.469255 1.067837 0.1922123 -0.4061871 9.030362 10.266150 4.403116 0.6162913 0.3505703 -1.219430 0.7185458 0.8260667 0.4667333 -0.3593333 0.01362890 0.0136289 FALSE TRUE
MSTRG.22005.2 ENSG00000100234 HEK293_OSMI2_6hA HEK293_TMG_6hB TIMP3 protein_coding   12.44853 12.55171 9.469255 1.067837 0.1922123 -0.4061871 3.418173 2.285558 5.066139 0.9811283 0.5273515 1.144887 0.2814542 0.1739333 0.5332667 0.3593333 0.01364928 0.0136289 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100234 E001 3.538542 0.0097542012 1.676812e-01 0.387493528 22 32801705 32801716 12 + 0.496 0.712 0.962
ENSG00000100234 E002 13.683583 0.0175030192 4.912895e-03 0.034162747 22 32801717 32801820 104 + 0.917 1.244 1.188
ENSG00000100234 E003 52.534304 0.0010014854 8.666471e-05 0.001326035 22 32801821 32802067 247 + 1.565 1.767 0.685
ENSG00000100234 E004 50.102093 0.0010386547 1.480171e-01 0.359486184 22 32802068 32802122 55 + 1.639 1.707 0.231
ENSG00000100234 E005 75.259685 0.0005411881 4.864508e-01 0.691524603 22 32849452 32849534 83 + 1.845 1.866 0.071
ENSG00000100234 E006 111.420689 0.0003807494 3.490994e-01 0.586462427 22 32857249 32857360 112 + 2.010 2.034 0.081
ENSG00000100234 E007 134.300108 0.0003654396 9.575484e-01 0.980653789 22 32858017 32858138 122 + 2.115 2.106 -0.033
ENSG00000100234 E008 1138.466677 0.0004850885 6.159877e-04 0.006719049 22 32859180 32863041 3862 + 3.052 3.026 -0.088