ENSG00000100226

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216044 ENSG00000100226 HEK293_OSMI2_6hA HEK293_TMG_6hB GTPBP1 protein_coding protein_coding 56.93551 85.94501 38.1439 10.50724 1.305787 -1.171751 47.764435 75.488039 28.983475 12.7157657 1.0313363 -1.3807109 0.81472083 0.86666667 0.75993333 -0.10673333 3.905161e-01 6.910357e-07 FALSE TRUE
ENST00000418601 ENSG00000100226 HEK293_OSMI2_6hA HEK293_TMG_6hB GTPBP1 protein_coding nonsense_mediated_decay 56.93551 85.94501 38.1439 10.50724 1.305787 -1.171751 3.376418 4.035089 3.227523 0.3468745 0.4034834 -0.3212809 0.07023333 0.04946667 0.08536667 0.03590000 4.362299e-01 6.910357e-07 FALSE FALSE
MSTRG.22133.2 ENSG00000100226 HEK293_OSMI2_6hA HEK293_TMG_6hB GTPBP1 protein_coding   56.93551 85.94501 38.1439 10.50724 1.305787 -1.171751 1.601999 1.414311 3.011882 0.1315439 0.2712906 1.0851837 0.03449583 0.01696667 0.07940000 0.06243333 6.910357e-07 6.910357e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100226 E001 1.2755951 0.1394855767 4.972874e-01   22 38705742 38705817 76 + 0.182 0.350 1.252
ENSG00000100226 E002 3.2588037 0.0447424698 3.177099e-01 5.576859e-01 22 38705934 38705943 10 + 0.408 0.628 1.061
ENSG00000100226 E003 3.4202194 0.0685751850 5.667518e-01 7.478263e-01 22 38705944 38705948 5 + 0.488 0.630 0.653
ENSG00000100226 E004 234.9031524 0.0066373302 2.274263e-01 4.622886e-01 22 38705949 38706117 169 + 2.249 2.348 0.329
ENSG00000100226 E005 185.5998311 0.0064578204 5.682239e-01 7.489584e-01 22 38706118 38706147 30 + 2.212 2.224 0.042
ENSG00000100226 E006 0.1426347 0.0316538257 1.660432e-01   22 38706511 38706519 9 + 0.181 0.000 -17.244
ENSG00000100226 E007 0.7603386 0.0679860101 1.217293e-01   22 38706520 38706781 262 + 0.409 0.145 -1.975
ENSG00000100226 E008 0.2617363 0.0876573999 7.134299e-01   22 38706782 38706856 75 + 0.000 0.145 15.447
ENSG00000100226 E009 357.7567871 0.0002394115 1.601835e-02 8.188234e-02 22 38708845 38708956 112 + 2.515 2.503 -0.040
ENSG00000100226 E010 583.2473526 0.0030840544 3.632341e-02 1.451150e-01 22 38715907 38716087 181 + 2.738 2.710 -0.092
ENSG00000100226 E011 1.4863530 0.1498822600 7.139965e-01 8.443246e-01 22 38716088 38716240 153 + 0.407 0.346 -0.348
ENSG00000100226 E012 894.1841100 0.0016708131 1.687258e-05 3.304142e-04 22 38716652 38717000 349 + 2.945 2.887 -0.195
ENSG00000100226 E013 525.3634717 0.0001640169 1.728597e-10 1.166291e-08 22 38721742 38721865 124 + 2.724 2.653 -0.237
ENSG00000100226 E014 539.0078394 0.0001241138 1.094613e-08 5.088706e-07 22 38724297 38724411 115 + 2.726 2.669 -0.190
ENSG00000100226 E015 3.0121055 0.0146798247 7.036190e-01 8.379221e-01 22 38725176 38725440 265 + 0.488 0.581 0.437
ENSG00000100226 E016 4.5874605 0.0132454062 4.985335e-01 6.997765e-01 22 38725807 38726005 199 + 0.754 0.682 -0.297
ENSG00000100226 E017 575.5200390 0.0002382260 2.287842e-04 2.987989e-03 22 38726006 38726150 145 + 2.731 2.706 -0.082
ENSG00000100226 E018 329.5573329 0.0009397578 3.202083e-01 5.599071e-01 22 38726258 38726305 48 + 2.462 2.475 0.043
ENSG00000100226 E019 453.5164866 0.0002029641 2.721453e-01 5.120150e-01 22 38726306 38726440 135 + 2.597 2.616 0.062
ENSG00000100226 E020 457.3470598 0.0001847137 4.602651e-03 3.248695e-02 22 38727213 38727348 136 + 2.623 2.609 -0.048
ENSG00000100226 E021 566.0048461 0.0008170835 3.702168e-01 6.041196e-01 22 38727983 38728161 179 + 2.692 2.710 0.061
ENSG00000100226 E022 18.0867148 0.0607788460 9.027459e-01 9.532186e-01 22 38728256 38729461 1206 + 1.195 1.243 0.169
ENSG00000100226 E023 527.8075064 0.0015905292 6.201895e-01 7.839524e-01 22 38729462 38729662 201 + 2.638 2.688 0.165
ENSG00000100226 E024 185.3902488 0.0005868280 9.645626e-01 9.841976e-01 22 38730612 38730612 1 + 2.191 2.231 0.135
ENSG00000100226 E025 245.4241559 0.0003226874 9.323706e-01 9.683392e-01 22 38730613 38730659 47 + 2.312 2.354 0.141
ENSG00000100226 E026 442.9319149 0.0005254880 3.294843e-01 5.684650e-01 22 38730660 38730881 222 + 2.555 2.615 0.200
ENSG00000100226 E027 3827.0733211 0.0037631325 3.281663e-06 8.017578e-05 22 38730882 38733587 2706 + 3.426 3.568 0.469
ENSG00000100226 E028 16.2669287 0.5301042000 6.590768e-01 8.094445e-01 22 38734150 38734232 83 + 1.373 1.099 -0.967
ENSG00000100226 E029 9.6945129 0.3886004912 2.744079e-01 5.143743e-01 22 38734233 38734323 91 + 1.253 0.820 -1.591
ENSG00000100226 E030 10.9976975 0.5008315312 4.423193e-01 6.602849e-01 22 38734324 38734324 1 + 1.255 0.912 -1.242
ENSG00000100226 E031 19.3466616 0.5932675600 6.808262e-01 8.231863e-01 22 38734325 38734397 73 + 1.442 1.170 -0.952
ENSG00000100226 E032 8.2291221 0.5659398077 7.600775e-01 8.725833e-01 22 38735174 38735310 137 + 0.997 0.895 -0.381
ENSG00000100226 E033 24.3877155 0.5303189828 9.961594e-01 9.997150e-01 22 38736029 38736256 228 + 1.435 1.325 -0.383
ENSG00000100226 E034 28.7115319 0.6046487122 9.495049e-01 9.767797e-01 22 38737951 38738299 349 + 1.486 1.401 -0.293