ENSG00000100225

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266087 ENSG00000100225 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXO7 protein_coding protein_coding 108.3062 110.2824 76.48415 14.4994 2.054757 -0.5279127 48.285195 53.172770 30.203262 6.4232973 0.3029360 -0.8157770 0.45557917 0.48350000 0.39543333 -0.0880666667 0.1184099524 0.0003456357 FALSE TRUE
ENST00000397426 ENSG00000100225 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXO7 protein_coding protein_coding 108.3062 110.2824 76.48415 14.4994 2.054757 -0.5279127 28.683348 23.129185 28.817173 3.7084351 1.5691265 0.3170913 0.26355833 0.20980000 0.37620000 0.1664000000 0.0003456357 0.0003456357 FALSE TRUE
ENST00000420700 ENSG00000100225 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXO7 protein_coding nonsense_mediated_decay 108.3062 110.2824 76.48415 14.4994 2.054757 -0.5279127 11.745975 15.973418 6.352855 2.0731859 0.1729065 -1.3288298 0.10698750 0.14516667 0.08326667 -0.0619000000 0.0040987427 0.0003456357 FALSE TRUE
ENST00000452138 ENSG00000100225 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXO7 protein_coding protein_coding 108.3062 110.2824 76.48415 14.4994 2.054757 -0.5279127 6.074581 4.343648 3.012725 0.5006604 0.3766869 -0.5263749 0.05150833 0.03963333 0.03916667 -0.0004666667 1.0000000000 0.0003456357 FALSE TRUE
MSTRG.22000.2 ENSG00000100225 HEK293_OSMI2_6hA HEK293_TMG_6hB FBXO7 protein_coding   108.3062 110.2824 76.48415 14.4994 2.054757 -0.5279127 8.537019 9.581306 5.717121 1.9476760 0.6737111 -0.7439172 0.08012500 0.08513333 0.07440000 -0.0107333333 0.8545275051 0.0003456357 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100225 E001 14.4837130 0.0023692877 1.660900e-01 3.852845e-01 22 32474676 32474724 49 + 1.082 1.215 0.474
ENSG00000100225 E002 54.6451520 0.0025224731 2.395454e-02 1.089632e-01 22 32474725 32474736 12 + 1.644 1.771 0.432
ENSG00000100225 E003 147.8085938 0.0003460934 1.335378e-08 6.084729e-07 22 32474737 32474797 61 + 2.026 2.211 0.619
ENSG00000100225 E004 143.9477130 0.0003206669 3.833738e-08 1.572601e-06 22 32474798 32474810 13 + 2.017 2.198 0.604
ENSG00000100225 E005 290.6518534 0.0013734259 8.495134e-12 7.402099e-10 22 32474811 32475014 204 + 2.308 2.507 0.663
ENSG00000100225 E006 241.8837292 0.0004312646 4.681116e-07 1.462132e-05 22 32475015 32475124 110 + 2.277 2.409 0.440
ENSG00000100225 E007 0.2922838 0.0268159122 6.826921e-01   22 32475125 32475236 112 + 0.154 0.095 -0.805
ENSG00000100225 E008 0.3337900 0.0299725354 6.861393e-01   22 32475237 32475251 15 + 0.154 0.095 -0.801
ENSG00000100225 E009 0.3337900 0.0299725354 6.861393e-01   22 32475252 32475254 3 + 0.154 0.095 -0.801
ENSG00000100225 E010 0.3337900 0.0299725354 6.861393e-01   22 32475255 32475256 2 + 0.154 0.095 -0.801
ENSG00000100225 E011 26.8056427 0.0013765545 5.923934e-01 7.651819e-01 22 32475257 32475398 142 + 1.395 1.434 0.133
ENSG00000100225 E012 0.5530924 0.0222251577 9.276922e-01   22 32475593 32475957 365 + 0.154 0.173 0.200
ENSG00000100225 E013 1.4458422 0.1718964026 1.327302e-01 3.360892e-01 22 32478035 32478196 162 + 0.549 0.241 -1.774
ENSG00000100225 E014 527.7238161 0.0005189540 1.521820e-01 3.655447e-01 22 32478981 32479132 152 + 2.718 2.693 -0.084
ENSG00000100225 E015 768.2112513 0.0003701438 1.611614e-04 2.232193e-03 22 32479133 32479275 143 + 2.900 2.844 -0.184
ENSG00000100225 E016 1065.7058744 0.0003045391 3.171197e-05 5.663879e-04 22 32483897 32484124 228 + 3.039 2.988 -0.171
ENSG00000100225 E017 746.2926948 0.0001628301 4.965970e-04 5.642906e-03 22 32485068 32485209 142 + 2.882 2.835 -0.156
ENSG00000100225 E018 6.5844083 0.1356902930 2.183524e-02 1.020342e-01 22 32487288 32487744 457 + 1.084 0.653 -1.672
ENSG00000100225 E019 520.9270688 0.0008962842 5.982645e-04 6.562763e-03 22 32487745 32487828 84 + 2.739 2.670 -0.228
ENSG00000100225 E020 46.6547149 0.0140460739 3.255797e-12 3.068309e-10 22 32487829 32491085 3257 + 1.921 1.380 -1.840
ENSG00000100225 E021 707.7277518 0.0001188362 1.211156e-01 3.176412e-01 22 32491086 32491181 96 + 2.842 2.822 -0.068
ENSG00000100225 E022 19.8892977 0.0904723197 3.930147e-03 2.875526e-02 22 32491182 32493104 1923 + 1.526 1.086 -1.542
ENSG00000100225 E023 949.1070620 0.0001538370 4.992987e-01 7.003258e-01 22 32493105 32493281 177 + 2.960 2.954 -0.022
ENSG00000100225 E024 418.7601432 0.0002040443 7.732728e-03 4.806189e-02 22 32495493 32495530 38 + 2.566 2.618 0.171
ENSG00000100225 E025 1710.7897396 0.0009248819 1.822204e-04 2.474034e-03 22 32498144 32498829 686 + 3.174 3.235 0.202