ENSG00000100221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216039 ENSG00000100221 HEK293_OSMI2_6hA HEK293_TMG_6hB JOSD1 protein_coding protein_coding 42.69615 48.06045 36.8822 2.780189 1.220986 -0.3818348 34.227549 41.08448358 28.612613 3.72741548 0.5956355 -0.5217893 0.7975583 0.8516667 0.7772667 -0.0744000 0.5667175846 0.0005042235 FALSE TRUE
ENST00000683374 ENSG00000100221 HEK293_OSMI2_6hA HEK293_TMG_6hB JOSD1 protein_coding protein_coding 42.69615 48.06045 36.8822 2.780189 1.220986 -0.3818348 3.543224 4.05048979 1.476393 0.58606442 0.6437373 -1.4498382 0.0828250 0.0860000 0.0392000 -0.0468000 0.5683499172 0.0005042235 FALSE TRUE
MSTRG.22125.7 ENSG00000100221 HEK293_OSMI2_6hA HEK293_TMG_6hB JOSD1 protein_coding   42.69615 48.06045 36.8822 2.780189 1.220986 -0.3818348 2.012440 0.09727709 5.333184 0.09727709 0.4930606 5.6382859 0.0507875 0.0022000 0.1441333 0.1419333 0.0005042235 0.0005042235 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100221 E001 1757.0672520 0.0008282637 1.812353e-04 2.462430e-03 22 38685543 38687089 1547 - 3.190 3.247 0.191
ENSG00000100221 E002 152.5091772 0.0007857125 5.800038e-03 3.874603e-02 22 38687090 38687139 50 - 2.101 2.198 0.324
ENSG00000100221 E003 328.2361022 0.0002826331 7.669775e-04 8.002114e-03 22 38687140 38687323 184 - 2.444 2.523 0.263
ENSG00000100221 E004 657.6020217 0.0012078680 6.723434e-01 8.178048e-01 22 38687324 38687717 394 - 2.798 2.800 0.008
ENSG00000100221 E005 452.6470056 0.0011132469 1.449486e-01 3.548719e-01 22 38687718 38687923 206 - 2.654 2.630 -0.079
ENSG00000100221 E006 274.3763409 0.0007592867 4.392039e-01 6.581393e-01 22 38687924 38688001 78 - 2.428 2.417 -0.037
ENSG00000100221 E007 319.3618911 0.0016978651 8.536826e-02 2.553224e-01 22 38688935 38689073 139 - 2.510 2.473 -0.124
ENSG00000100221 E008 216.0865207 0.0003500534 7.174838e-03 4.545955e-02 22 38689074 38689129 56 - 2.356 2.294 -0.205
ENSG00000100221 E009 10.1169994 0.0093909945 7.671847e-01 8.769072e-01 22 38689130 38689295 166 - 1.044 1.019 -0.092
ENSG00000100221 E010 307.5015707 0.0002562437 7.971440e-03 4.918019e-02 22 38689296 38689424 129 - 2.501 2.452 -0.164
ENSG00000100221 E011 0.5059767 0.0211440432 2.025253e-01   22 38699801 38699802 2 - 0.000 0.242 10.933
ENSG00000100221 E012 478.3327498 0.0001264077 9.857517e-08 3.663092e-06 22 38699803 38700123 321 - 2.710 2.629 -0.269
ENSG00000100221 E013 158.6597989 0.0002616793 3.029378e-02 1.282440e-01 22 38700124 38700160 37 - 2.218 2.162 -0.188
ENSG00000100221 E014 166.2697405 0.0002510349 8.668468e-02 2.576674e-01 22 38700161 38700275 115 - 2.228 2.186 -0.140
ENSG00000100221 E015 46.9060921 0.0008662385 8.176979e-02 2.483349e-01 22 38700276 38700317 42 - 1.714 1.628 -0.289
ENSG00000100221 E016 181.1428585 0.0003667146 6.036761e-01 7.725991e-01 22 38700318 38700618 301 - 2.226 2.248 0.073
ENSG00000100221 E017 34.6975629 0.0017193432 1.862850e-05 3.596985e-04 22 38700619 38700667 49 - 1.316 1.617 1.036
ENSG00000100221 E018 12.4850219 0.0054119310 6.778801e-03 4.360923e-02 22 38700800 38700861 62 - 0.909 1.208 1.089
ENSG00000100221 E019 8.8164827 0.0461256346 3.478959e-02 1.410378e-01 22 38700862 38700903 42 - 0.747 1.081 1.272
ENSG00000100221 E020 8.8776798 0.0038235037 1.104536e-03 1.070331e-02 22 38700904 38700943 40 - 0.678 1.094 1.600
ENSG00000100221 E021 2.2489339 0.0892169453 3.524438e-03 2.648952e-02 22 38700966 38701256 291 - 0.000 0.633 12.129
ENSG00000100221 E022 12.1064198 0.0032591295 2.735286e-01 5.133891e-01 22 38701257 38702055 799 - 1.026 1.144 0.425