ENSG00000100220

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000216038 ENSG00000100220 HEK293_OSMI2_6hA HEK293_TMG_6hB RTCB protein_coding protein_coding 144.6655 120.086 149.7914 10.04921 8.893496 0.3188627 139.7657 118.6238 138.2601 10.37352 8.123368 0.2209744 0.9640875 0.9872 0.9230667 -0.06413333 0.0005052876 1.121705e-09 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000100220 E001 133.849169 7.446105e-03 5.358463e-01 7.262166e-01 22 32387582 32387651 70 - 2.137 2.116 -0.070
ENSG00000100220 E002 136.874109 5.550933e-03 2.716875e-01 5.115277e-01 22 32387652 32387652 1 - 2.157 2.115 -0.142
ENSG00000100220 E003 1603.239523 1.148718e-03 2.797184e-05 5.075067e-04 22 32387653 32388099 447 - 3.161 3.242 0.271
ENSG00000100220 E004 1090.649495 7.381269e-04 1.779624e-03 1.556150e-02 22 32392240 32392359 120 - 3.005 3.063 0.193
ENSG00000100220 E005 1038.345384 2.974974e-04 4.582984e-04 5.297975e-03 22 32393892 32394002 111 - 2.987 3.039 0.172
ENSG00000100220 E006 1408.247630 1.677023e-04 3.325697e-02 1.367450e-01 22 32395026 32395214 189 - 3.132 3.160 0.092
ENSG00000100220 E007 1269.902247 1.305957e-04 3.732134e-01 6.066876e-01 22 32396074 32396249 176 - 3.095 3.109 0.047
ENSG00000100220 E008 515.638475 2.662285e-04 1.681554e-01 3.881046e-01 22 32397941 32397947 7 - 2.698 2.726 0.093
ENSG00000100220 E009 833.758599 7.546039e-04 9.393309e-01 9.719184e-01 22 32397948 32398041 94 - 2.919 2.924 0.014
ENSG00000100220 E010 398.024964 1.669563e-03 6.915380e-01 8.300896e-01 22 32398042 32398047 6 - 2.604 2.599 -0.017
ENSG00000100220 E011 652.390289 1.693420e-03 1.170617e-01 3.109517e-01 22 32398048 32398100 53 - 2.831 2.800 -0.103
ENSG00000100220 E012 1150.664482 7.703455e-04 8.119610e-04 8.380443e-03 22 32399603 32399759 157 - 3.085 3.037 -0.159
ENSG00000100220 E013 19.900913 2.017099e-03 5.000583e-09 2.513955e-07 22 32399760 32399985 226 - 1.503 1.026 -1.682
ENSG00000100220 E014 18.772293 3.058463e-03 6.075573e-15 9.195829e-13 22 32401562 32401746 185 - 1.525 0.809 -2.578
ENSG00000100220 E015 1019.972823 8.441847e-05 2.180423e-05 4.110178e-04 22 32401747 32401903 157 - 3.030 2.985 -0.147
ENSG00000100220 E016 742.641004 2.318719e-04 7.622678e-02 2.371777e-01 22 32406662 32406761 100 - 2.880 2.859 -0.070
ENSG00000100220 E017 1.194400 2.744390e-02 1.576230e-01   22 32407313 32407405 93 - 0.469 0.215 -1.601
ENSG00000100220 E018 1.213181 1.582537e-02 1.486443e-01   22 32407406 32407526 121 - 0.469 0.215 -1.604
ENSG00000100220 E019 2.146819 2.082276e-02 1.046798e-02 6.014506e-02 22 32407879 32408174 296 - 0.661 0.215 -2.480
ENSG00000100220 E020 583.971569 2.064305e-04 6.431377e-01 7.989317e-01 22 32408175 32408242 68 - 2.760 2.772 0.039
ENSG00000100220 E021 349.210098 1.607615e-04 5.837946e-01 7.594757e-01 22 32408755 32408757 3 - 2.545 2.539 -0.022
ENSG00000100220 E022 370.074189 1.850496e-04 1.907279e-01 4.178112e-01 22 32408758 32408760 3 - 2.578 2.557 -0.070
ENSG00000100220 E023 771.088158 1.208879e-04 9.419287e-04 9.436680e-03 22 32408761 32408833 73 - 2.906 2.866 -0.133
ENSG00000100220 E024 797.163598 1.525028e-04 2.330524e-04 3.035562e-03 22 32412064 32412248 185 - 2.923 2.878 -0.149