ENSG00000099995

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215793 ENSG00000099995 HEK293_OSMI2_6hA HEK293_TMG_6hB SF3A1 protein_coding protein_coding 106.7727 144.9021 98.85066 24.46928 4.69466 -0.5517097 71.37385 99.26414 55.93591 18.796571 1.656057 -0.8273855 0.6681458 0.6790000 0.5669 -0.11210000 0.08893516 0.02181431 FALSE TRUE
MSTRG.21927.5 ENSG00000099995 HEK293_OSMI2_6hA HEK293_TMG_6hB SF3A1 protein_coding   106.7727 144.9021 98.85066 24.46928 4.69466 -0.5517097 32.47674 43.67201 38.43496 6.773247 1.728464 -0.1842450 0.3024375 0.3036333 0.3889 0.08526667 0.02181431 0.02181431 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000099995 E001 134.594990 3.602314e-03 9.614900e-01 9.827807e-01 22 30331988 30332245 258 - 2.102 2.105 0.012
ENSG00000099995 E002 3949.415872 1.798856e-04 9.411757e-06 1.989708e-04 22 30332246 30334695 2450 - 3.542 3.582 0.130
ENSG00000099995 E003 999.917009 8.171478e-05 2.153838e-04 2.845804e-03 22 30335467 30335538 72 - 2.939 2.990 0.171
ENSG00000099995 E004 1080.588147 1.251091e-04 6.047670e-04 6.620897e-03 22 30335652 30335753 102 - 2.975 3.023 0.157
ENSG00000099995 E005 918.477609 9.739406e-04 5.756037e-02 1.973021e-01 22 30337026 30337180 155 - 2.908 2.949 0.135
ENSG00000099995 E006 575.647977 6.335509e-03 9.214832e-02 2.677031e-01 22 30337690 30337800 111 - 2.681 2.757 0.254
ENSG00000099995 E007 383.393096 6.252863e-03 5.911069e-02 2.007791e-01 22 30337801 30337816 16 - 2.495 2.585 0.299
ENSG00000099995 E008 659.640619 1.974266e-03 7.304116e-02 2.309152e-01 22 30337817 30337897 81 - 2.757 2.810 0.176
ENSG00000099995 E009 648.540700 1.595415e-04 1.321580e-02 7.131478e-02 22 30338789 30338839 51 - 2.757 2.802 0.148
ENSG00000099995 E010 1016.480496 4.756706e-04 7.208924e-03 4.561770e-02 22 30338840 30338960 121 - 2.949 2.997 0.158
ENSG00000099995 E011 867.617665 3.941949e-04 2.018352e-02 9.652564e-02 22 30338961 30339034 74 - 2.885 2.927 0.139
ENSG00000099995 E012 6.474974 9.182436e-02 9.025296e-01 9.531276e-01 22 30339035 30339129 95 - 0.841 0.853 0.050
ENSG00000099995 E013 860.870806 1.458313e-04 6.828364e-01 8.244615e-01 22 30339130 30339179 50 - 2.903 2.914 0.034
ENSG00000099995 E014 950.957135 5.769363e-04 5.387417e-01 7.281196e-01 22 30339180 30339251 72 - 2.957 2.949 -0.027
ENSG00000099995 E015 581.153318 1.802912e-04 8.844871e-02 2.609061e-01 22 30340196 30340208 13 - 2.755 2.731 -0.080
ENSG00000099995 E016 1324.044513 7.938667e-05 4.608199e-01 6.737424e-01 22 30340209 30340381 173 - 3.098 3.095 -0.011
ENSG00000099995 E017 4.071880 5.451414e-02 3.750916e-01 6.082577e-01 22 30340382 30340694 313 - 0.780 0.623 -0.652
ENSG00000099995 E018 980.828879 8.511662e-05 2.959943e-01 5.364922e-01 22 30340695 30340812 118 - 2.972 2.964 -0.026
ENSG00000099995 E019 1594.481854 1.099371e-04 3.433935e-07 1.111630e-05 22 30341692 30341885 194 - 3.207 3.160 -0.156
ENSG00000099995 E020 1494.152322 1.504198e-03 5.985197e-05 9.716319e-04 22 30342200 30342319 120 - 3.197 3.119 -0.258
ENSG00000099995 E021 862.904966 1.889927e-03 1.991611e-04 2.666099e-03 22 30342320 30342350 31 - 2.964 2.878 -0.287
ENSG00000099995 E022 8.219055 1.602573e-01 8.033576e-02 2.455209e-01 22 30342492 30342546 55 - 1.147 0.782 -1.368
ENSG00000099995 E023 7.948143 3.301366e-01 1.410919e-01 3.491636e-01 22 30342547 30342574 28 - 1.130 0.784 -1.297
ENSG00000099995 E024 14.301429 1.230073e-01 2.670142e-01 5.066290e-01 22 30342575 30342804 230 - 1.277 1.089 -0.667
ENSG00000099995 E025 1009.753572 9.565666e-04 5.537950e-06 1.260179e-04 22 30342805 30342879 75 - 3.029 2.948 -0.269
ENSG00000099995 E026 2.154381 4.144210e-01 8.556169e-02 2.556239e-01 22 30344910 30344932 23 - 0.714 0.286 -2.159
ENSG00000099995 E027 1631.465161 5.575964e-05 9.878811e-14 1.231528e-11 22 30344933 30345190 258 - 3.229 3.164 -0.216
ENSG00000099995 E028 4.904855 1.749321e-02 2.637676e-01 5.030287e-01 22 30346221 30346311 91 - 0.624 0.795 0.710
ENSG00000099995 E029 1129.976649 8.234847e-04 6.782088e-03 4.362376e-02 22 30346312 30346519 208 - 3.056 3.013 -0.144
ENSG00000099995 E030 0.446685 2.295705e-02 2.036978e-02   22 30352545 30352950 406 - 0.375 0.000 -11.003
ENSG00000099995 E031 743.054925 2.971330e-02 3.729973e-01 6.065121e-01 22 30352951 30353072 122 - 2.801 2.863 0.208
ENSG00000099995 E032 478.231577 1.536265e+00 4.488881e-01 6.649078e-01 22 30356730 30356919 190 - 2.466 2.721 0.848