ENSG00000099804

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215574 ENSG00000099804 HEK293_OSMI2_6hA HEK293_TMG_6hB CDC34 protein_coding protein_coding 176.3326 269.9642 77.81428 63.40248 2.309529 -1.79453 124.605316 204.16842 58.330303 50.475253 1.93252757 -1.8072656 0.71977083 0.74926667 0.7497000 0.0004333333 1.000000000 9.683209e-12 FALSE TRUE
ENST00000586283 ENSG00000099804 HEK293_OSMI2_6hA HEK293_TMG_6hB CDC34 protein_coding protein_coding 176.3326 269.9642 77.81428 63.40248 2.309529 -1.79453 11.135318 18.51135 4.745638 4.625120 0.84976903 -1.9614787 0.06077083 0.06793333 0.0605000 -0.0074333333 0.900494068 9.683209e-12 FALSE FALSE
MSTRG.15990.5 ENSG00000099804 HEK293_OSMI2_6hA HEK293_TMG_6hB CDC34 protein_coding   176.3326 269.9642 77.81428 63.40248 2.309529 -1.79453 9.781515 13.95334 8.186441 2.249374 0.07776462 -0.7685748 0.05712500 0.05430000 0.1053667 0.0510666667 0.004901927 9.683209e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000099804 E001 0.767713 0.0176292369 0.3069619171   19 531665 531704 40 + 0.001 0.256 9.146
ENSG00000099804 E002 1.434918 0.0127128726 0.0977136613 0.277774757 19 531705 531720 16 + 0.000 0.388 11.962
ENSG00000099804 E003 20.223787 0.0207560068 0.0022004310 0.018410789 19 531721 531759 39 + 0.874 1.299 1.544
ENSG00000099804 E004 20.890991 0.0162409180 0.0010077885 0.009971959 19 531760 531768 9 + 0.874 1.314 1.597
ENSG00000099804 E005 39.842059 0.0790019568 0.0019515229 0.016743973 19 531769 531818 50 + 1.003 1.586 2.048
ENSG00000099804 E006 404.152543 0.0001375845 0.0018477078 0.016041270 19 531819 531994 176 + 2.448 2.528 0.265
ENSG00000099804 E007 504.702392 0.0001427603 0.0700649651 0.224931333 19 531995 532031 37 + 2.572 2.617 0.148
ENSG00000099804 E008 879.796152 0.0001182835 0.1126997021 0.303653898 19 532032 532099 68 + 2.862 2.846 -0.053
ENSG00000099804 E009 644.622578 0.0001438849 0.1591254388 0.375469244 19 532100 532108 9 + 2.729 2.711 -0.057
ENSG00000099804 E010 1194.608475 0.0001112809 0.0015272627 0.013801114 19 535837 535923 87 + 3.008 2.974 -0.112
ENSG00000099804 E011 942.267425 0.0001144267 0.0050035342 0.034646899 19 536243 536278 36 + 2.905 2.871 -0.112
ENSG00000099804 E012 1143.482904 0.0001052973 0.3017524982 0.542291575 19 536279 536340 62 + 2.968 2.961 -0.023
ENSG00000099804 E013 18.498200 0.0978448933 0.9908901998 0.997173066 19 536341 536715 375 + 1.202 1.191 -0.041
ENSG00000099804 E014 10.959378 0.0222086250 0.7008218214 0.836185467 19 536953 537012 60 + 1.031 0.976 -0.203
ENSG00000099804 E015 1874.641460 0.0002060084 0.1944501133 0.422650441 19 537013 537147 135 + 3.183 3.173 -0.034
ENSG00000099804 E016 133.761434 0.0043278254 0.1204506464 0.316557153 19 538678 539024 347 + 2.094 2.016 -0.260
ENSG00000099804 E017 28.480986 0.0303139205 0.5147548613 0.711494569 19 541217 541252 36 + 1.441 1.353 -0.304
ENSG00000099804 E018 795.575859 0.0001189866 0.4330375507 0.653512063 19 541339 541342 4 + 2.810 2.804 -0.019
ENSG00000099804 E019 830.823233 0.0001472853 0.5048019649 0.704169342 19 541343 541345 3 + 2.827 2.823 -0.014
ENSG00000099804 E020 979.494690 0.0001172228 0.4151795440 0.639912883 19 541346 541360 15 + 2.900 2.895 -0.017
ENSG00000099804 E021 3317.729015 0.0004465116 0.0001077652 0.001591531 19 541361 542092 732 + 3.385 3.430 0.150