ENSG00000099625

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000215376 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding retained_intron 2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.18650175 0.2094757 0.11688364 0.1066008 0.05967963 -0.7905553 0.0621250 0.05293333 0.07830000 0.02536667 9.427808e-01 3.177287e-18 TRUE FALSE
ENST00000590083 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding protein_coding 2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.75409807 1.2101149 0.46538224 0.1232940 0.07776675 -1.3598571 0.2689792 0.33783333 0.30730000 -0.03053333 9.708228e-01 3.177287e-18 FALSE TRUE
ENST00000591127 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding nonsense_mediated_decay 2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.83399962 0.9117992 0.00000000 0.6272022 0.00000000 -6.5263807 0.2436875 0.20476667 0.00000000 -0.20476667 4.680401e-01 3.177287e-18 TRUE TRUE
ENST00000648750 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding nonsense_mediated_decay 2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.16394823 0.4139797 0.07123908 0.2309903 0.04948728 -2.3837494 0.0527375 0.10300000 0.04646667 -0.05653333 8.652258e-01 3.177287e-18 TRUE TRUE
ENST00000650044 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding protein_coding 2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.58548445 0.4056615 0.00000000 0.2043988 0.00000000 -5.3773374 0.1747250 0.10186667 0.00000000 -0.10186667 4.768281e-01 3.177287e-18 FALSE TRUE
MSTRG.16044.5 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding   2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.08257119 0.5122063 0.00000000 0.2561724 0.00000000 -5.7065479 0.0265875 0.15860000 0.00000000 -0.15860000 5.074447e-01 3.177287e-18 FALSE FALSE
MSTRG.16044.6 ENSG00000099625 HEK293_OSMI2_6hA HEK293_TMG_6hB CBARP protein_coding   2.94941 3.78825 1.522431 0.5557785 0.06189789 -1.309513 0.30542559 0.0000000 0.86892639 0.0000000 0.09125512 6.4576704 0.1579458 0.00000000 0.56786667 0.56786667 3.177287e-18 3.177287e-18 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000099625 E001 37.0058247 0.001019401 3.206836e-05 0.0005715728 19 1228287 1229178 892 - 1.241 1.553 1.078
ENSG00000099625 E002 18.1462897 0.015529904 4.270850e-03 0.0306662962 19 1229179 1229695 517 - 0.919 1.268 1.263
ENSG00000099625 E003 6.6093895 0.018303661 2.655765e-01 0.5049767933 19 1229696 1229947 252 - 0.668 0.844 0.711
ENSG00000099625 E004 8.8541468 0.003404005 7.626633e-02 0.2372599490 19 1229948 1230137 190 - 0.721 0.964 0.946
ENSG00000099625 E005 3.6302893 0.006695349 8.986117e-02 0.2635299790 19 1230138 1230142 5 - 0.346 0.662 1.560
ENSG00000099625 E006 25.4756762 0.014257442 1.767769e-01 0.3994491898 19 1230143 1231100 958 - 1.436 1.308 -0.444
ENSG00000099625 E007 21.5130192 0.006239902 4.361805e-01 0.6559412647 19 1231101 1231275 175 - 1.336 1.263 -0.254
ENSG00000099625 E008 36.5035979 0.010345074 1.624298e-01 0.3803735541 19 1233426 1233636 211 - 1.581 1.472 -0.372
ENSG00000099625 E009 30.1202777 0.026088048 5.369481e-01 0.7269843100 19 1234191 1234290 100 - 1.467 1.392 -0.258
ENSG00000099625 E010 15.1953061 0.002552937 8.435694e-01 0.9213885131 19 1234291 1234331 41 - 1.120 1.135 0.056
ENSG00000099625 E011 15.3129188 0.002307106 2.874870e-01 0.5282321662 19 1234571 1234573 3 - 1.055 1.161 0.384
ENSG00000099625 E012 27.1235363 0.001314440 6.507480e-01 0.8040264223 19 1234574 1234646 73 - 1.348 1.378 0.105
ENSG00000099625 E013 26.2562294 0.003629272 5.392400e-01 0.7284450839 19 1234647 1234742 96 - 1.393 1.337 -0.194
ENSG00000099625 E014 0.6673924 0.019132168 8.933670e-01   19 1234743 1234827 85 - 0.206 0.180 -0.245
ENSG00000099625 E015 13.0610034 0.054634676 9.952202e-01 0.9991622160 19 1235001 1235007 7 - 1.074 1.062 -0.043
ENSG00000099625 E016 25.4276319 0.007367055 1.539166e-01 0.3679261254 19 1235008 1235145 138 - 1.433 1.306 -0.441
ENSG00000099625 E017 6.3953443 0.004929053 6.737683e-01 0.8187985033 19 1235146 1235336 191 - 0.850 0.787 -0.246
ENSG00000099625 E018 3.9919366 0.008124701 9.031069e-01 0.9534142955 19 1235337 1235342 6 - 0.667 0.644 -0.097
ENSG00000099625 E019 4.7704361 0.011613261 6.184965e-01 0.7827970392 19 1235343 1235390 48 - 0.769 0.691 -0.316
ENSG00000099625 E020 22.4616620 0.002216827 3.708677e-02 0.1471366979 19 1235501 1235565 65 - 1.414 1.242 -0.602
ENSG00000099625 E021 26.8176554 0.001613081 2.948208e-01 0.5353479855 19 1235779 1235914 136 - 1.424 1.340 -0.292
ENSG00000099625 E022 11.4358115 0.006352908 5.311176e-01 0.7228619160 19 1235915 1235918 4 - 1.077 1.003 -0.273
ENSG00000099625 E023 16.0144431 0.002159944 7.860857e-01 0.8881505872 19 1235996 1236121 126 - 1.139 1.161 0.079
ENSG00000099625 E024 5.7355636 0.004869372 8.991442e-02 0.2636178337 19 1237756 1238027 272 - 0.536 0.811 1.167