ENSG00000088881

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380648 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding protein_coding 4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 0.3083813 0.2511884 0.07687037 0.25118837 0.07687037 -1.5881547 0.05103750 0.03283333 0.05513333 0.02230000 0.9404585816 0.0005114974 FALSE TRUE
ENST00000449079 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding nonsense_mediated_decay 4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 0.5207506 1.1046097 0.00000000 0.34132250 0.00000000 -6.8003948 0.09496250 0.15380000 0.00000000 -0.15380000 0.0005114974 0.0005114974 TRUE TRUE
ENST00000477287 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding processed_transcript 4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 1.6931990 2.1357113 0.49022700 0.03556991 0.18593143 -2.1008012 0.33294583 0.30576667 0.27956667 -0.02620000 0.9542590238 0.0005114974   FALSE
ENST00000609451 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding protein_coding 4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 1.3270721 2.1646539 0.66561734 0.53264748 0.10843139 -1.6865075 0.28103333 0.31126667 0.40600000 0.09473333 0.8157743763 0.0005114974 FALSE TRUE
MSTRG.20261.3 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding   4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 0.2820348 0.2176740 0.16277147 0.15394772 0.16277147 -0.3981043 0.07295417 0.03223333 0.07890000 0.04666667 1.0000000000 0.0005114974 FALSE FALSE
MSTRG.20261.6 ENSG00000088881 HEK293_OSMI2_6hA HEK293_TMG_6hB EBF4 protein_coding   4.960442 7.020843 1.689157 0.3286388 0.1971465 -2.048879 0.7230563 1.0310814 0.19151407 0.13906560 0.01012574 -2.3691303 0.14337917 0.14640000 0.11700000 -0.02940000 0.8408854184 0.0005114974 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000088881 E001 0.1308682 0.0326491905 1.0000000000   20 2692874 2692877 4 + 0.001 0.054 6.782
ENSG00000088881 E002 0.1308682 0.0326491905 1.0000000000   20 2692878 2692878 1 + 0.000 0.054 8.837
ENSG00000088881 E003 0.2617363 0.1077103340 1.0000000000   20 2692879 2692950 72 + 0.000 0.101 9.549
ENSG00000088881 E004 3.9677883 0.2334093982 0.5638223393 0.745983772 20 2692951 2693148 198 + 0.413 0.610 0.951
ENSG00000088881 E005 0.3088520 0.0263656723 0.1970381099   20 2693574 2693660 87 + 0.251 0.054 -2.561
ENSG00000088881 E006 13.8917229 0.0443609292 0.3928032708 0.622662042 20 2693661 2693782 122 + 0.908 1.054 0.541
ENSG00000088881 E007 20.9922771 0.0038924242 0.2351686113 0.471093042 20 2705577 2705667 91 + 1.297 1.185 -0.393
ENSG00000088881 E008 23.3388956 0.0020151206 0.1084691501 0.296473816 20 2705668 2705733 66 + 1.360 1.219 -0.496
ENSG00000088881 E009 25.7318767 0.0014940545 0.2688102113 0.508551847 20 2705974 2706037 64 + 1.360 1.265 -0.331
ENSG00000088881 E010 26.8112435 0.0017279819 0.1016838476 0.284794934 20 2706209 2706264 56 + 1.415 1.280 -0.470
ENSG00000088881 E011 32.5921991 0.0015959542 0.0988152544 0.279719014 20 2707947 2708020 74 + 1.487 1.363 -0.431
ENSG00000088881 E012 26.9628214 0.0123902322 0.4858720350 0.691167327 20 2709574 2709642 69 + 1.359 1.287 -0.248
ENSG00000088881 E013 24.0547198 0.0085326591 0.3225777454 0.561967402 20 2748549 2748630 82 + 1.329 1.231 -0.343
ENSG00000088881 E014 1.3446460 0.0174119377 0.5328620755   20 2749187 2749400 214 + 0.409 0.285 -0.753
ENSG00000088881 E015 22.8328684 0.0241731190 0.8843525406 0.943628508 20 2749401 2749518 118 + 1.241 1.227 -0.050
ENSG00000088881 E016 27.6425571 0.0023956157 0.5878777117 0.762258639 20 2749620 2749753 134 + 1.260 1.313 0.184
ENSG00000088881 E017 25.4092869 0.0020815303 0.4733593956 0.682362397 20 2749847 2749973 127 + 1.329 1.268 -0.215
ENSG00000088881 E018 23.0924783 0.0063282433 0.7991278658 0.895771589 20 2751700 2751788 89 + 1.261 1.240 -0.072
ENSG00000088881 E019 13.4730288 0.0026201728 0.0041667837 0.030092099 20 2751922 2751987 66 + 0.616 1.066 1.765
ENSG00000088881 E020 12.1547985 0.0026577690 0.0005366673 0.006009789 20 2752086 2752263 178 + 0.409 1.030 2.633
ENSG00000088881 E021 7.4770867 0.0046647727 0.0291761771 0.124893777 20 2752357 2752495 139 + 0.409 0.828 1.872
ENSG00000088881 E022 8.9998137 0.0457496509 0.0191979609 0.093140050 20 2752496 2752545 50 + 0.409 0.899 2.147
ENSG00000088881 E023 17.6562675 0.0432391104 0.6637399865 0.812249769 20 2755088 2755626 539 + 1.178 1.115 -0.224
ENSG00000088881 E024 8.4587481 0.0280740842 0.3614042600 0.596856517 20 2755627 2755649 23 + 0.691 0.849 0.634
ENSG00000088881 E025 15.7585498 0.0091481894 0.0812278825 0.247311819 20 2755650 2755742 93 + 0.861 1.101 0.891
ENSG00000088881 E026 23.1351275 0.0020987114 0.2436930512 0.480708611 20 2755743 2755824 82 + 1.131 1.255 0.441
ENSG00000088881 E027 28.4284100 0.0014575050 0.7213109575 0.848974471 20 2758909 2758984 76 + 1.296 1.331 0.123
ENSG00000088881 E028 95.2594928 0.0006589475 0.8702747119 0.936077920 20 2759268 2759882 615 + 1.839 1.835 -0.016
ENSG00000088881 E029 18.6923001 0.0019977842 0.6123798231 0.778457393 20 2759883 2760108 226 + 1.105 1.163 0.208