ENSG00000086475

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000327347 ENSG00000086475 HEK293_OSMI2_6hA HEK293_TMG_6hB SEPHS1 protein_coding protein_coding 51.49169 37.25227 58.60088 3.499116 3.270688 0.653453 10.39247 4.343206 13.31218 1.2815811 0.1939121 1.61367973 0.1940292 0.1152333 0.2283000 0.1130667 2.974830e-01 1.806005e-06 FALSE TRUE
MSTRG.3559.3 ENSG00000086475 HEK293_OSMI2_6hA HEK293_TMG_6hB SEPHS1 protein_coding   51.49169 37.25227 58.60088 3.499116 3.270688 0.653453 11.67550 7.765702 16.17485 0.4076889 1.1633671 1.05759948 0.2237125 0.2110333 0.2756333 0.0646000 3.496922e-01 1.806005e-06 FALSE TRUE
MSTRG.3559.7 ENSG00000086475 HEK293_OSMI2_6hA HEK293_TMG_6hB SEPHS1 protein_coding   51.49169 37.25227 58.60088 3.499116 3.270688 0.653453 23.49058 21.424919 21.15121 2.5568913 1.8246715 -0.01854105 0.4670583 0.5727333 0.3596333 -0.2131000 1.806005e-06 1.806005e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000086475 E001 0.8440911 0.3919485080 5.509258e-01   10 13317428 13317438 11 - 0.192 0.379 1.328
ENSG00000086475 E002 144.1403741 0.0173169968 2.272029e-03 1.887500e-02 10 13317439 13318079 641 - 2.236 2.024 -0.709
ENSG00000086475 E003 186.9731024 0.0039363581 2.710982e-09 1.444578e-07 10 13318080 13318563 484 - 2.359 2.113 -0.821
ENSG00000086475 E004 116.2692037 0.0009889227 1.001635e-17 2.192019e-15 10 13318564 13318669 106 - 2.180 1.849 -1.112
ENSG00000086475 E005 268.4627731 0.0006192740 1.860821e-42 3.668280e-39 10 13318670 13319034 365 - 2.547 2.188 -1.198
ENSG00000086475 E006 525.8168410 0.0002831790 2.443371e-01 4.814535e-01 10 13319035 13319356 322 - 2.710 2.723 0.043
ENSG00000086475 E007 544.9425999 0.0001754180 2.052298e-09 1.128286e-07 10 13322835 13323041 207 - 2.692 2.778 0.286
ENSG00000086475 E008 200.2055301 0.0003181711 6.635607e-04 7.126731e-03 10 13323042 13323047 6 - 2.262 2.342 0.269
ENSG00000086475 E009 417.4465159 0.0006132563 1.112401e-03 1.076540e-02 10 13328351 13328450 100 - 2.590 2.650 0.198
ENSG00000086475 E010 367.2874545 0.0002688652 2.144950e-02 1.008114e-01 10 13329698 13329788 91 - 2.543 2.581 0.126
ENSG00000086475 E011 299.6867998 0.0002302439 6.133959e-01 7.792161e-01 10 13333817 13333876 60 - 2.470 2.474 0.013
ENSG00000086475 E012 272.2349869 0.0007545481 9.654125e-01 9.846033e-01 10 13333877 13333900 24 - 2.432 2.427 -0.016
ENSG00000086475 E013 420.0250245 0.0001789306 4.278606e-01 6.493950e-01 10 13333901 13333971 71 - 2.613 2.620 0.024
ENSG00000086475 E014 478.5063060 0.0006881761 6.049052e-01 7.734289e-01 10 13336243 13336350 108 - 2.671 2.676 0.017
ENSG00000086475 E015 281.7024535 0.0002555757 5.652601e-01 7.468767e-01 10 13338705 13338715 11 - 2.441 2.447 0.019
ENSG00000086475 E016 468.1235792 0.0003861854 4.365659e-01 6.561664e-01 10 13338716 13338808 93 - 2.659 2.667 0.027
ENSG00000086475 E017 14.6280830 0.0021683571 6.295761e-02 2.093650e-01 10 13340691 13340908 218 - 1.112 1.270 0.560
ENSG00000086475 E018 545.3180694 0.0004019803 3.098034e-03 2.400608e-02 10 13344758 13345028 271 - 2.709 2.754 0.148
ENSG00000086475 E019 17.6687571 0.0392784841 9.229652e-01 9.638070e-01 10 13345029 13345460 432 - 1.257 1.273 0.056
ENSG00000086475 E020 14.9382088 0.0143252395 1.068157e-05 2.221921e-04 10 13347277 13347629 353 - 0.942 1.394 1.618
ENSG00000086475 E021 73.0675026 0.0007911275 6.858011e-02 2.218757e-01 10 13348000 13348298 299 - 1.827 1.898 0.239