ENSG00000085978

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392017 ENSG00000085978 HEK293_OSMI2_6hA HEK293_TMG_6hB ATG16L1 protein_coding protein_coding 18.5773 16.11909 20.69903 1.241 0.3158806 0.3605952 2.9474876 3.7164483 4.022262 1.58610263 0.26239136 0.1137882 0.16199583 0.22496667 0.1945667 -0.03040000 1.000000e+00 1.632889e-14 FALSE TRUE
ENST00000392020 ENSG00000085978 HEK293_OSMI2_6hA HEK293_TMG_6hB ATG16L1 protein_coding protein_coding 18.5773 16.11909 20.69903 1.241 0.3158806 0.3605952 6.0718347 4.3928553 6.312599 0.20945510 0.37482229 0.5220782 0.32622917 0.27573333 0.3052667 0.02953333 8.755393e-01 1.632889e-14 FALSE TRUE
ENST00000473865 ENSG00000085978 HEK293_OSMI2_6hA HEK293_TMG_6hB ATG16L1 protein_coding retained_intron 18.5773 16.11909 20.69903 1.241 0.3158806 0.3605952 2.9356593 4.1949480 0.000000 0.97046034 0.00000000 -8.7159441 0.16554167 0.26206667 0.0000000 -0.26206667 1.632889e-14 1.632889e-14 FALSE FALSE
ENST00000479942 ENSG00000085978 HEK293_OSMI2_6hA HEK293_TMG_6hB ATG16L1 protein_coding retained_intron 18.5773 16.11909 20.69903 1.241 0.3158806 0.3605952 3.3734640 1.2060120 5.346167 0.31595463 0.68697980 2.1390436 0.17510417 0.07480000 0.2579333 0.18313333 3.821578e-03 1.632889e-14 TRUE TRUE
MSTRG.20082.8 ENSG00000085978 HEK293_OSMI2_6hA HEK293_TMG_6hB ATG16L1 protein_coding   18.5773 16.11909 20.69903 1.241 0.3158806 0.3605952 0.7882895 0.3547371 1.649184 0.03753871 0.05549702 2.1855452 0.04154583 0.02226667 0.0796000 0.05733333 5.592987e-05 1.632889e-14 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000085978 E001 0.0000000       2 233210051 233210079 29 +      
ENSG00000085978 E002 0.0000000       2 233210327 233210583 257 +      
ENSG00000085978 E003 1.9992651 0.0119331660 3.473396e-02 1.408997e-01 2 233251571 233251669 99 + 0.598 0.248 -1.944
ENSG00000085978 E004 3.1236810 0.0955177514 5.371771e-01 7.271012e-01 2 233251670 233251671 2 + 0.651 0.568 -0.364
ENSG00000085978 E005 3.1236810 0.0955177514 5.371771e-01 7.271012e-01 2 233251672 233251672 1 + 0.651 0.568 -0.364
ENSG00000085978 E006 3.5891470 0.1090449882 3.356528e-01 5.741264e-01 2 233251673 233251675 3 + 0.722 0.568 -0.661
ENSG00000085978 E007 4.3449737 0.0127485297 5.303917e-01 7.223385e-01 2 233251676 233251681 6 + 0.747 0.687 -0.245
ENSG00000085978 E008 10.3968634 0.0031252500 2.075829e-01 4.389097e-01 2 233251682 233251684 3 + 1.091 0.994 -0.353
ENSG00000085978 E009 23.4430220 0.0016899620 1.513155e-02 7.858844e-02 2 233251685 233251700 16 + 1.434 1.290 -0.499
ENSG00000085978 E010 25.0559915 0.0015684006 2.936741e-02 1.254752e-01 2 233251701 233251704 4 + 1.451 1.332 -0.414
ENSG00000085978 E011 24.9251233 0.0016037055 2.280742e-02 1.053394e-01 2 233251705 233251705 1 + 1.451 1.324 -0.441
ENSG00000085978 E012 27.5192090 0.0015171088 9.114336e-02 2.657619e-01 2 233251706 233251713 8 + 1.475 1.397 -0.271
ENSG00000085978 E013 84.6110380 0.0006144744 6.758731e-03 4.351143e-02 2 233251714 233251940 227 + 1.948 1.878 -0.236
ENSG00000085978 E014 32.3064079 0.0011521347 1.117809e-01 3.020460e-01 2 233251941 233251942 2 + 1.534 1.471 -0.217
ENSG00000085978 E015 86.9789302 0.0004640379 1.673323e-03 1.483502e-02 2 233256102 233256195 94 + 1.965 1.880 -0.285
ENSG00000085978 E016 106.1882111 0.0004777919 1.239060e-06 3.420921e-05 2 233263130 233263235 106 + 2.069 1.932 -0.458
ENSG00000085978 E017 87.3734196 0.0004921017 9.708098e-06 2.043707e-04 2 233263992 233264065 74 + 1.985 1.848 -0.459
ENSG00000085978 E018 0.3503582 0.0288513029 8.460155e-01   2 233264066 233264101 36 + 0.104 0.142 0.514
ENSG00000085978 E019 134.2486756 0.0011390901 4.908829e-05 8.188115e-04 2 233264892 233265143 252 + 2.159 2.058 -0.336
ENSG00000085978 E020 0.0000000       2 233265955 233266159 205 +      
ENSG00000085978 E021 68.8426231 0.0027807404 6.949141e-01 8.322454e-01 2 233270002 233270067 66 + 1.827 1.846 0.062
ENSG00000085978 E022 78.3953536 0.0018249564 8.589289e-02 2.562422e-01 2 233272966 233273052 87 + 1.903 1.866 -0.127
ENSG00000085978 E023 8.0670774 0.0176997975 2.650656e-01 5.043925e-01 2 233273053 233273720 668 + 0.985 0.880 -0.398
ENSG00000085978 E024 30.8749143 0.0026854056 3.736802e-02 1.479154e-01 2 233273721 233273777 57 + 1.527 1.424 -0.355
ENSG00000085978 E025 1.9789859 0.1348883169 8.797252e-01 9.410995e-01 2 233273991 233274041 51 + 0.463 0.468 0.023
ENSG00000085978 E026 4.1410221 0.3309321362 1.439387e-01 3.533049e-01 2 233274304 233274675 372 + 0.800 0.529 -1.154
ENSG00000085978 E027 99.7271395 0.0049930596 1.441763e-01 3.536376e-01 2 233274676 233274778 103 + 2.002 1.974 -0.094
ENSG00000085978 E028 0.3206185 0.0274424043 2.823946e-01   2 233275379 233275467 89 + 0.187 0.000 -10.167
ENSG00000085978 E029 0.7804750 0.0209263996 2.804661e-01   2 233277148 233277567 420 + 0.318 0.142 -1.483
ENSG00000085978 E030 85.3988991 0.0119045652 5.285522e-01 7.209984e-01 2 233277568 233277610 43 + 1.922 1.929 0.026
ENSG00000085978 E031 94.3115657 0.0136820326 3.935474e-01 6.231843e-01 2 233277611 233277673 63 + 1.968 1.966 -0.005
ENSG00000085978 E032 70.2949850 0.0073351387 4.414316e-01 6.596962e-01 2 233281105 233281146 42 + 1.838 1.840 0.007
ENSG00000085978 E033 68.7812369 0.0011392120 5.797866e-01 7.570778e-01 2 233281147 233281175 29 + 1.823 1.837 0.046
ENSG00000085978 E034 100.3054113 0.0037869236 2.693189e-01 5.091172e-01 2 233282682 233282753 72 + 1.995 1.987 -0.025
ENSG00000085978 E035 0.8102147 0.1097611274 8.826114e-01   2 233282754 233282995 242 + 0.257 0.249 -0.059
ENSG00000085978 E036 125.0889763 0.0005662090 1.088177e-01 2.970926e-01 2 233289854 233289970 117 + 2.091 2.077 -0.048
ENSG00000085978 E037 42.2505496 0.0021434066 3.191272e-01 5.588429e-01 2 233289971 233289974 4 + 1.627 1.607 -0.067
ENSG00000085978 E038 24.1040999 0.0159329256 1.916249e-03 1.651643e-02 2 233289975 233290106 132 + 1.480 1.227 -0.881
ENSG00000085978 E039 31.2261183 0.0393465616 2.551257e-02 1.137903e-01 2 233290107 233290247 141 + 1.575 1.368 -0.710
ENSG00000085978 E040 110.4127146 0.0004629670 6.499776e-01 8.035551e-01 2 233290248 233290353 106 + 2.010 2.063 0.180
ENSG00000085978 E041 57.8742727 0.0008341281 6.224561e-01 7.855057e-01 2 233292128 233292132 5 + 1.753 1.767 0.048
ENSG00000085978 E042 153.8914806 0.0011773479 5.976613e-04 6.557477e-03 2 233292133 233292277 145 + 2.112 2.259 0.494
ENSG00000085978 E043 107.8972773 0.0066162145 6.262933e-03 4.101300e-02 2 233292387 233292434 48 + 1.946 2.124 0.597
ENSG00000085978 E044 139.2330536 0.0026558210 1.786298e-02 8.857163e-02 2 233293256 233293357 102 + 2.078 2.207 0.433
ENSG00000085978 E045 640.3277537 0.0056067680 4.307834e-08 1.743077e-06 2 233294257 233295674 1418 + 2.696 2.913 0.722