ENSG00000085274

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000349841 ENSG00000085274 HEK293_OSMI2_6hA HEK293_TMG_6hB MYNN protein_coding protein_coding 6.998481 1.252365 14.65804 0.1333458 1.046835 3.538474 2.361052 0.3430838 4.533224 0.16213485 0.3742930 3.685634 0.3279750 0.3018667 0.3122000 0.01033333 0.958323730 0.004736914 FALSE TRUE
ENST00000356716 ENSG00000085274 HEK293_OSMI2_6hA HEK293_TMG_6hB MYNN protein_coding protein_coding 6.998481 1.252365 14.65804 0.1333458 1.046835 3.538474 1.183474 0.4925821 2.345699 0.08828907 0.2148130 2.228724 0.2035042 0.3879667 0.1605000 -0.22746667 0.004736914 0.004736914 FALSE TRUE
ENST00000602391 ENSG00000085274 HEK293_OSMI2_6hA HEK293_TMG_6hB MYNN protein_coding nonsense_mediated_decay 6.998481 1.252365 14.65804 0.1333458 1.046835 3.538474 3.220827 0.3916571 7.487829 0.20095097 0.9041269 4.222437 0.4317375 0.2892000 0.5075667 0.21836667 0.730658301 0.004736914 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000085274 E001 7.887492 0.031243707 0.64705878 0.80149963 3 169773396 169773404 9 + 0.772 0.907 0.523
ENSG00000085274 E002 23.203929 0.028523318 0.03135808 0.13138241 3 169773405 169773420 16 + 1.228 0.993 -0.848
ENSG00000085274 E003 25.980462 0.025695361 0.02113512 0.09978108 3 169773421 169773426 6 + 1.274 1.031 -0.870
ENSG00000085274 E004 40.307002 0.019221842 0.05420771 0.18957406 3 169773427 169773462 36 + 1.448 1.312 -0.470
ENSG00000085274 E005 20.895429 0.052490873 0.90201586 0.95291832 3 169774031 169774097 67 + 1.147 1.237 0.317
ENSG00000085274 E006 89.736025 0.003301941 0.02393268 0.10888846 3 169774265 169774561 297 + 1.783 1.712 -0.240
ENSG00000085274 E007 32.246531 0.730629154 0.33758622 0.57597155 3 169778768 169778883 116 + 1.305 1.545 0.830
ENSG00000085274 E008 96.978016 1.357368770 0.51076672 0.70857085 3 169778884 169779288 405 + 1.782 1.953 0.576
ENSG00000085274 E009 39.567550 0.866587726 0.58209338 0.75842875 3 169779289 169779333 45 + 1.414 1.517 0.354
ENSG00000085274 E010 82.078040 1.341525035 0.65688313 0.80788714 3 169779334 169779561 228 + 1.727 1.792 0.218
ENSG00000085274 E011 67.340153 0.008883647 0.59745816 0.76861179 3 169780590 169780749 160 + 1.644 1.738 0.320
ENSG00000085274 E012 90.008688 0.023088539 0.55242716 0.73799057 3 169782465 169782643 179 + 1.770 1.796 0.088
ENSG00000085274 E013 52.923372 0.057038943 0.88634213 0.94475976 3 169783477 169783560 84 + 1.540 1.603 0.217
ENSG00000085274 E014 4.037190 0.006604438 0.70035101 0.83585662 3 169783730 169783858 129 + 0.540 0.657 0.521
ENSG00000085274 E015 60.272259 0.058214794 0.96790557 0.98580090 3 169784622 169784708 87 + 1.593 1.667 0.251
ENSG00000085274 E016 149.534284 0.043448601 0.73199982 0.85541223 3 169786416 169789716 3301 + 1.976 2.092 0.388