ENSG00000084676

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000288599 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding protein_coding 6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 2.0773636 2.39067374 3.25499509 0.49861802 0.202200120 0.4436415 0.33432917 0.50643333 0.409933333 -0.09650000 6.173090e-01 2.044294e-09 FALSE TRUE
ENST00000348332 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding protein_coding 6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.7943952 0.34829875 1.30148543 0.15983991 0.456991197 1.8719669 0.11620000 0.06420000 0.160033333 0.09583333 5.148040e-01 2.044294e-09 FALSE TRUE
ENST00000395856 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding protein_coding 6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.4660796 0.25478229 0.82481004 0.10227158 0.203173443 1.6566413 0.08482917 0.04960000 0.103066667 0.05346667 4.914855e-01 2.044294e-09 FALSE TRUE
ENST00000407230 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding protein_coding 6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.2178571 0.23019326 0.00000000 0.12553019 0.000000000 -4.5861238 0.04473333 0.05793333 0.000000000 -0.05793333 2.103134e-01 2.044294e-09 FALSE TRUE
ENST00000493773 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding retained_intron 6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.4041679 0.09580157 0.01819065 0.04875555 0.009292707 -1.9080723 0.08083333 0.01813333 0.002366667 -0.01576667 2.535240e-01 2.044294e-09 FALSE FALSE
MSTRG.18053.12 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding   6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.3659664 0.00000000 1.03691795 0.00000000 0.122642630 6.7100046 0.04545417 0.00000000 0.129533333 0.12953333 2.044294e-09 2.044294e-09 FALSE TRUE
MSTRG.18053.15 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding   6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.6967332 1.19653957 0.60012882 0.46865697 0.372288662 -0.9836894 0.12214583 0.23990000 0.080166667 -0.15973333 5.405739e-01 2.044294e-09 FALSE TRUE
MSTRG.18053.4 ENSG00000084676 HEK293_OSMI2_6hA HEK293_TMG_6hB NCOA1 protein_coding   6.086102 4.773037 7.988287 1.044926 0.3932366 0.741764 0.3201795 0.00000000 0.38615407 0.00000000 0.386154068 5.3079897 0.05408750 0.00000000 0.046866667 0.04686667 9.260073e-01 2.044294e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000084676 E001 2.8349001 0.0081629908 7.295640e-01 8.539740e-01 2 24491254 24491291 38 + 0.605 0.562 -0.195
ENSG00000084676 E002 4.5732864 0.0059951597 9.376011e-02 2.707451e-01 2 24491292 24491369 78 + 0.831 0.604 -0.935
ENSG00000084676 E003 5.0565380 0.0078755427 3.318029e-01 5.706507e-01 2 24491370 24491545 176 + 0.831 0.712 -0.478
ENSG00000084676 E004 2.5080749 0.0147854465 1.500075e-01 3.623305e-01 2 24491546 24491602 57 + 0.633 0.400 -1.124
ENSG00000084676 E005 0.3088520 0.0268668204 8.407449e-01   2 24491914 24491931 18 + 0.106 0.139 0.454
ENSG00000084676 E006 0.4812263 0.0220584353 4.148025e-01   2 24491932 24492022 91 + 0.106 0.244 1.456
ENSG00000084676 E007 0.1723744 0.0645905405 3.676433e-01   2 24492023 24492057 35 + 0.000 0.140 11.508
ENSG00000084676 E008 0.0000000       2 24492058 24492188 131 +      
ENSG00000084676 E009 0.1308682 0.0326491905 3.695511e-01   2 24554386 24554388 3 + 0.000 0.139 11.739
ENSG00000084676 E010 0.2617363 0.0896806349 1.502883e-01   2 24554389 24554421 33 + 0.000 0.242 12.336
ENSG00000084676 E011 0.7567544 0.0210541434 9.805449e-02   2 24554422 24554502 81 + 0.106 0.399 2.454
ENSG00000084676 E012 0.7567544 0.0210541434 9.805449e-02   2 24554503 24554520 18 + 0.106 0.399 2.454
ENSG00000084676 E013 1.6562112 0.0120053376 8.565825e-01 9.285898e-01 2 24554521 24554573 53 + 0.423 0.400 -0.125
ENSG00000084676 E014 1.7387462 0.0114839864 2.939463e-01 5.345946e-01 2 24556015 24556055 41 + 0.505 0.329 -0.955
ENSG00000084676 E015 0.6190593 0.0247985952 4.739310e-01   2 24563930 24563961 32 + 0.261 0.139 -1.123
ENSG00000084676 E016 0.9223018 0.0592817257 7.174137e-01   2 24563962 24564013 52 + 0.261 0.329 0.462
ENSG00000084676 E017 3.5446066 0.0075831783 5.443276e-01 7.321754e-01 2 24564295 24564309 15 + 0.686 0.605 -0.348
ENSG00000084676 E018 14.6491332 0.0081986338 2.285504e-01 4.635786e-01 2 24564310 24564430 121 + 1.224 1.118 -0.375
ENSG00000084676 E019 7.7786794 0.0042667717 7.153297e-02 2.279036e-01 2 24584476 24584476 1 + 1.004 0.799 -0.777
ENSG00000084676 E020 16.3113502 0.0024081677 4.955712e-02 1.785497e-01 2 24584477 24584540 64 + 1.289 1.130 -0.562
ENSG00000084676 E021 13.4787332 0.0026747732 3.597495e-02 1.441522e-01 2 24584541 24584560 20 + 1.222 1.035 -0.673
ENSG00000084676 E022 33.8398449 0.0011979003 2.693064e-03 2.153222e-02 2 24643966 24644122 157 + 1.606 1.433 -0.593
ENSG00000084676 E023 33.5041314 0.0023830384 6.330289e-02 2.101430e-01 2 24658661 24658766 106 + 1.581 1.478 -0.355
ENSG00000084676 E024 0.6600180 0.0193874923 7.091245e-02   2 24658767 24659129 363 + 0.323 0.000 -14.069
ENSG00000084676 E025 50.2899898 0.0009962588 2.469222e-02 1.112696e-01 2 24665749 24665915 167 + 1.751 1.651 -0.337
ENSG00000084676 E026 41.2248557 0.0011240687 1.635951e-03 1.457083e-02 2 24673366 24673463 98 + 1.684 1.520 -0.560
ENSG00000084676 E027 50.4996513 0.0021803781 3.231371e-04 3.965208e-03 2 24682951 24683128 178 + 1.778 1.596 -0.615
ENSG00000084676 E028 53.5721526 0.0127581414 1.794341e-02 8.885146e-02 2 24691481 24691660 180 + 1.798 1.636 -0.551
ENSG00000084676 E029 36.2053344 0.0049554880 3.387351e-04 4.127207e-03 2 24693252 24693347 96 + 1.656 1.425 -0.791
ENSG00000084676 E030 0.0000000       2 24693768 24693797 30 +      
ENSG00000084676 E031 41.2653770 0.0012221160 1.324887e-01 3.357401e-01 2 24697658 24697798 141 + 1.651 1.581 -0.238
ENSG00000084676 E032 45.4647444 0.0011537824 1.880268e-01 4.144605e-01 2 24705086 24705233 148 + 1.687 1.630 -0.194
ENSG00000084676 E033 34.6239065 0.0065162046 7.469737e-01 8.648951e-01 2 24706568 24706625 58 + 1.550 1.536 -0.047
ENSG00000084676 E034 190.6165121 0.0035889684 9.599637e-02 2.747193e-01 2 24706626 24707726 1101 + 2.299 2.249 -0.170
ENSG00000084676 E035 18.0692429 0.0552394395 4.630032e-01 6.751612e-01 2 24707727 24707730 4 + 1.301 1.237 -0.227
ENSG00000084676 E036 57.6343203 0.0014653933 2.047853e-01 4.356727e-01 2 24707731 24707888 158 + 1.779 1.732 -0.159
ENSG00000084676 E037 63.7578478 0.0008120064 3.699207e-01 6.038931e-01 2 24710931 24711111 181 + 1.817 1.792 -0.087
ENSG00000084676 E038 0.1614157 0.0341483629 6.462311e-01   2 24711112 24711483 372 + 0.106 0.000 -12.002
ENSG00000084676 E039 45.6286900 0.0008520493 8.565293e-01 9.285683e-01 2 24726589 24726706 118 + 1.654 1.677 0.079
ENSG00000084676 E040 60.7426679 0.0007020236 7.918618e-01 8.915165e-01 2 24728308 24728476 169 + 1.773 1.799 0.088
ENSG00000084676 E041 86.0821845 0.0008525776 8.778787e-01 9.401719e-01 2 24729501 24729815 315 + 1.929 1.951 0.073
ENSG00000084676 E042 43.9288395 0.0015538893 2.443120e-01 4.814423e-01 2 24739432 24739533 102 + 1.615 1.693 0.268
ENSG00000084676 E043 95.6632243 0.0029481220 7.493299e-01 8.663534e-01 2 24741784 24742186 403 + 1.972 2.003 0.104
ENSG00000084676 E044 66.1257441 0.0076048806 6.888309e-02 2.225501e-01 2 24751982 24752156 175 + 1.761 1.888 0.426
ENSG00000084676 E045 79.4647427 0.0018633127 1.463722e-02 7.672262e-02 2 24757973 24758156 184 + 1.840 1.962 0.408
ENSG00000084676 E046 49.8155408 0.0053475998 2.563449e-01 4.951125e-01 2 24762687 24762776 90 + 1.659 1.740 0.274
ENSG00000084676 E047 1.5714632 0.0135277122 4.554696e-01 6.698745e-01 2 24767771 24768078 308 + 0.323 0.462 0.783
ENSG00000084676 E048 24.5044268 0.0018613729 6.490309e-01 8.028756e-01 2 24768079 24768135 57 + 1.382 1.427 0.155
ENSG00000084676 E049 21.4996259 0.0034318633 1.409841e-01 3.490189e-01 2 24768221 24768223 3 + 1.282 1.409 0.441
ENSG00000084676 E050 309.9479350 0.0028437376 1.788000e-11 1.474714e-09 2 24768224 24770702 2479 + 2.384 2.595 0.704