ENSG00000077254

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370792 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding protein_coding 31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 3.310445 0.1969988 4.999433 0.1094278 1.0202754 4.596953 0.06506667 0.02123333 0.08763333 0.06640000 0.359164064 0.001923047 FALSE TRUE
ENST00000370794 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding protein_coding 31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 13.114098 2.4607231 24.388047 0.2158377 2.9731426 3.303760 0.37158750 0.25893333 0.42603333 0.16710000 0.241460500 0.001923047 FALSE TRUE
ENST00000481579 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding protein_coding 31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 2.584930 1.6531359 4.194157 0.1732714 0.1531556 1.337910 0.14179583 0.17516667 0.07313333 -0.10203333 0.001923047 0.001923047 FALSE TRUE
ENST00000533928 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding retained_intron 31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 1.328467 0.9185194 2.107509 0.2026813 0.3582405 1.189364 0.06150833 0.09616667 0.03670000 -0.05946667 0.089876117 0.001923047   FALSE
MSTRG.1447.5 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding   31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 3.730261 1.6102530 7.404106 0.6001215 1.7907796 2.194054 0.11888750 0.17540000 0.12796667 -0.04743333 0.954450209 0.001923047 FALSE TRUE
MSTRG.1447.8 ENSG00000077254 HEK293_OSMI2_6hA HEK293_TMG_6hB USP33 protein_coding   31.69747 9.465917 57.4852 0.3789081 1.226915 2.601104 2.242074 0.2900687 6.580799 0.2900687 0.3423927 4.457089 0.04362917 0.02883333 0.11433333 0.08550000 0.272327378 0.001923047 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000077254 E001 0.5352391 0.2304387287 2.421715e-02   1 77695987 77695990 4 - 0.053 0.516 4.125
ENSG00000077254 E002 45.0337534 0.0018143644 4.155048e-05 7.110265e-04 1 77695991 77696074 84 - 1.465 1.738 0.931
ENSG00000077254 E003 204.1989368 0.0002564542 9.942509e-06 2.086330e-04 1 77696075 77696321 247 - 2.137 2.286 0.497
ENSG00000077254 E004 510.7275457 0.0004998740 4.460287e-10 2.763882e-08 1 77696322 77697052 731 - 2.534 2.677 0.475
ENSG00000077254 E005 56.2856560 0.0007559517 1.947844e-01 4.230630e-01 1 77697053 77697074 22 - 1.597 1.685 0.297
ENSG00000077254 E006 293.5527210 0.0002133560 1.162344e-07 4.245918e-06 1 77697075 77697474 400 - 2.294 2.442 0.495
ENSG00000077254 E007 17.2922613 0.0654700488 1.422251e-03 1.305502e-02 1 77697609 77697862 254 - 1.005 1.484 1.695
ENSG00000077254 E008 142.4639252 0.0004376102 9.454243e-05 1.426266e-03 1 77697863 77697931 69 - 1.981 2.139 0.527
ENSG00000077254 E009 148.7577763 0.0133681077 1.294343e-01 3.309085e-01 1 77701369 77701471 103 - 2.008 2.119 0.371
ENSG00000077254 E010 5.1791335 0.0063795155 2.792463e-04 3.521905e-03 1 77701472 77701651 180 - 0.528 1.053 2.118
ENSG00000077254 E011 1.0534597 0.0269523068 3.984420e-06   1 77711502 77711651 150 - 0.000 0.761 15.139
ENSG00000077254 E012 1.2376005 0.3015438802 1.337808e-03   1 77711652 77711664 13 - 0.053 0.776 5.254
ENSG00000077254 E013 5.7842836 0.0052415102 1.032348e-04 1.536413e-03 1 77711665 77711746 82 - 0.576 1.109 2.102
ENSG00000077254 E014 118.6597758 0.0139939227 1.336469e-02 7.190125e-02 1 77711747 77711784 38 - 1.897 2.076 0.601
ENSG00000077254 E015 147.1738780 0.0029828848 2.584283e-03 2.086794e-02 1 77711785 77711855 71 - 1.998 2.139 0.473
ENSG00000077254 E016 161.9332952 0.0003036671 7.075389e-05 1.119609e-03 1 77713200 77713281 82 - 2.036 2.186 0.502
ENSG00000077254 E017 0.9764321 0.0313867526 4.471405e-02   1 77713282 77713508 227 - 0.145 0.528 2.582
ENSG00000077254 E018 231.4853523 0.0037131163 2.633890e-02 1.163044e-01 1 77714614 77714783 170 - 2.200 2.297 0.323
ENSG00000077254 E019 2.3793630 0.0099185623 2.255280e-01 4.599698e-01 1 77714784 77714917 134 - 0.389 0.619 1.127
ENSG00000077254 E020 185.2493679 0.0003344767 7.174186e-01 8.465395e-01 1 77715742 77715868 127 - 2.120 2.113 -0.023
ENSG00000077254 E021 0.3228314 0.3684910290 1.000000e+00   1 77717540 77717644 105 - 0.102 0.000 -9.497
ENSG00000077254 E022 1.1024985 0.1048081943 9.242736e-02   1 77717645 77717777 133 - 0.184 0.527 2.170
ENSG00000077254 E023 1.5360120 0.0121989077 6.381149e-01 7.958125e-01 1 77717778 77717866 89 - 0.312 0.412 0.586
ENSG00000077254 E024 234.5669005 0.0004219750 5.788804e-01 7.564213e-01 1 77717867 77718023 157 - 2.222 2.210 -0.041
ENSG00000077254 E025 101.9848181 0.0005149130 4.315718e-01 6.524018e-01 1 77718024 77718047 24 - 1.859 1.903 0.149
ENSG00000077254 E026 129.4320784 0.0003653186 5.916707e-01 7.648806e-01 1 77718596 77718641 46 - 1.969 1.952 -0.057
ENSG00000077254 E027 0.1426347 0.0314638100 1.000000e+00   1 77718642 77718712 71 - 0.054 0.000 -9.414
ENSG00000077254 E028 111.9365206 0.0003951361 4.080817e-02 1.568230e-01 1 77721172 77721205 34 - 1.917 1.823 -0.317
ENSG00000077254 E029 13.0877279 0.0029003951 9.596225e-01 9.817091e-01 1 77721206 77721738 533 - 1.009 1.021 0.043
ENSG00000077254 E030 148.7666213 0.0003681579 2.593527e-01 4.982867e-01 1 77721831 77721901 71 - 2.033 1.992 -0.136
ENSG00000077254 E031 59.7217290 0.0007351079 8.584558e-01 9.295509e-01 1 77721902 77721925 24 - 1.637 1.632 -0.017
ENSG00000077254 E032 235.8111706 0.0012479521 1.854322e-01 4.111320e-01 1 77722024 77722196 173 - 2.230 2.188 -0.140
ENSG00000077254 E033 13.2124391 0.0024489014 7.611738e-01 8.732870e-01 1 77722197 77722399 203 - 1.009 1.052 0.157
ENSG00000077254 E034 234.2048933 0.0002021525 1.207222e-01 3.169960e-01 1 77723331 77723443 113 - 2.227 2.182 -0.150
ENSG00000077254 E035 242.3764484 0.0003304138 2.581985e-02 1.147344e-01 1 77725622 77725762 141 - 2.245 2.177 -0.228
ENSG00000077254 E036 398.4276235 0.0026930754 1.217583e-01 3.186476e-01 1 77728295 77728712 418 - 2.458 2.409 -0.161
ENSG00000077254 E037 137.1557795 0.0112741344 2.842147e-02 1.225785e-01 1 77729860 77729933 74 - 2.010 1.863 -0.493
ENSG00000077254 E038 83.3851516 0.0067582102 2.794198e-02 1.211184e-01 1 77729934 77729938 5 - 1.795 1.641 -0.522
ENSG00000077254 E039 170.3188421 0.0002587927 1.244361e-02 6.822257e-02 1 77730618 77730731 114 - 2.096 2.002 -0.313
ENSG00000077254 E040 87.1309476 0.0004523707 6.373416e-01 7.952742e-01 1 77734347 77734356 10 - 1.799 1.780 -0.063
ENSG00000077254 E041 110.2019801 0.0003612030 3.014236e-01 5.419132e-01 1 77734357 77734416 60 - 1.902 1.858 -0.147
ENSG00000077254 E042 189.0993562 0.0003527425 8.290730e-05 1.279612e-03 1 77736056 77736158 103 - 2.148 1.999 -0.500
ENSG00000077254 E043 149.2726869 0.0005461321 1.743716e-06 4.624755e-05 1 77739265 77739310 46 - 2.054 1.839 -0.725
ENSG00000077254 E044 126.5346339 0.0034852041 1.606956e-03 1.437516e-02 1 77739311 77739330 20 - 1.979 1.807 -0.578
ENSG00000077254 E045 136.2472444 0.0013686895 1.098568e-03 1.066008e-02 1 77739331 77739376 46 - 2.007 1.853 -0.519
ENSG00000077254 E046 119.6866822 0.0004698834 5.459328e-05 8.981104e-04 1 77739377 77739397 21 - 1.956 1.757 -0.670
ENSG00000077254 E047 105.6564095 0.0004907123 6.713284e-04 7.194687e-03 1 77739398 77739417 20 - 1.900 1.726 -0.590
ENSG00000077254 E048 131.1530639 0.0007344218 1.947460e-04 2.618140e-03 1 77740877 77740934 58 - 1.993 1.818 -0.590
ENSG00000077254 E049 69.6168082 0.0006549301 1.111508e-02 6.284865e-02 1 77740935 77740939 5 - 1.720 1.563 -0.535
ENSG00000077254 E050 117.9034037 0.0003467564 3.923925e-03 2.872544e-02 1 77741376 77741429 54 - 1.943 1.808 -0.455
ENSG00000077254 E051 153.5399945 0.0004757142 1.068080e-02 6.102910e-02 1 77741617 77741748 132 - 2.051 1.948 -0.347
ENSG00000077254 E052 48.6173515 0.0007430332 4.160389e-02 1.588957e-01 1 77745421 77745599 179 - 1.568 1.420 -0.508
ENSG00000077254 E053 0.4514866 0.0217681645 3.813714e-01   1 77745600 77745638 39 - 0.102 0.253 1.583
ENSG00000077254 E054 1.9566436 0.1653107271 7.973987e-01 8.948315e-01 1 77746399 77746556 158 - 0.362 0.418 0.311
ENSG00000077254 E055 0.0000000       1 77759348 77759642 295 -      
ENSG00000077254 E056 78.2174851 0.0019850245 1.248301e-01 3.234614e-01 1 77759643 77759700 58 - 1.761 1.671 -0.304
ENSG00000077254 E057 78.2137858 0.0018489410 1.550685e-01 3.695449e-01 1 77759701 77759852 152 - 1.760 1.678 -0.276