ENSG00000077097

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264331 ENSG00000077097 HEK293_OSMI2_6hA HEK293_TMG_6hB TOP2B protein_coding protein_coding 69.89524 39.53286 120.5478 8.7709 4.742384 1.608236 8.884733 3.430994 14.11874 0.8444727 0.1595981 2.037736 0.1117083 0.08916667 0.1175333 0.02836667 6.380596e-01 9.212054e-14 FALSE TRUE
ENST00000413971 ENSG00000077097 HEK293_OSMI2_6hA HEK293_TMG_6hB TOP2B protein_coding nonsense_mediated_decay 69.89524 39.53286 120.5478 8.7709 4.742384 1.608236 31.989758 24.024759 51.74368 6.2285083 2.8430827 1.106539 0.5229708 0.59330000 0.4287333 -0.16456667 5.650923e-02 9.212054e-14 FALSE TRUE
ENST00000424225 ENSG00000077097 HEK293_OSMI2_6hA HEK293_TMG_6hB TOP2B protein_coding protein_coding 69.89524 39.53286 120.5478 8.7709 4.742384 1.608236 17.798177 7.247322 42.24334 1.9120119 2.2673255 2.541556 0.2135500 0.17926667 0.3502000 0.17093333 1.567568e-05 9.212054e-14 FALSE TRUE
ENST00000435706 ENSG00000077097 HEK293_OSMI2_6hA HEK293_TMG_6hB TOP2B protein_coding protein_coding 69.89524 39.53286 120.5478 8.7709 4.742384 1.608236 6.291286 3.792760 0.00000 0.6973483 0.0000000 -8.570903 0.0986250 0.11150000 0.0000000 -0.11150000 9.212054e-14 9.212054e-14 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000077097 E001 1.2615137 0.0128911895 1.777807e-01   3 25597954 25597983 30 - 0.250 0.496 1.453
ENSG00000077097 E002 6.2030983 0.0268306447 5.978347e-03 3.961993e-02 3 25597984 25597985 2 - 0.654 1.051 1.545
ENSG00000077097 E003 8.0312562 0.0045892964 2.248616e-04 2.946048e-03 3 25597986 25597989 4 - 0.739 1.173 1.630
ENSG00000077097 E004 101.1702739 0.0017878081 3.575923e-10 2.259203e-08 3 25597990 25598068 79 - 1.870 2.135 0.892
ENSG00000077097 E005 741.9871282 0.0012376874 7.757942e-26 4.277523e-23 3 25598069 25598477 409 - 2.746 2.976 0.766
ENSG00000077097 E006 506.9033515 0.0002330480 2.633057e-20 8.085960e-18 3 25599435 25599529 95 - 2.604 2.777 0.575
ENSG00000077097 E007 537.1939679 0.0001527406 5.650137e-27 3.527659e-24 3 25601100 25601225 126 - 2.623 2.811 0.627
ENSG00000077097 E008 365.8644445 0.0015087749 5.402112e-13 5.828480e-11 3 25604760 25604870 111 - 2.450 2.654 0.680
ENSG00000077097 E009 295.6320016 0.0028843157 5.758203e-09 2.848457e-07 3 25606043 25606122 80 - 2.356 2.562 0.685
ENSG00000077097 E010 572.0166554 0.0003642347 1.322403e-11 1.114191e-09 3 25607171 25607375 205 - 2.671 2.800 0.429
ENSG00000077097 E011 491.2125809 0.0014725928 4.591833e-05 7.735333e-04 3 25609183 25609344 162 - 2.612 2.720 0.361
ENSG00000077097 E012 489.4918660 0.0003398853 2.971362e-06 7.376197e-05 3 25609568 25609712 145 - 2.614 2.711 0.322
ENSG00000077097 E013 313.6648413 0.0001814483 7.816839e-03 4.846760e-02 3 25612515 25612570 56 - 2.429 2.497 0.227
ENSG00000077097 E014 460.0466074 0.0001229605 3.774919e-05 6.564285e-04 3 25612571 25612709 139 - 2.591 2.674 0.279
ENSG00000077097 E015 344.7652474 0.0001463802 3.545241e-05 6.220306e-04 3 25615205 25615288 84 - 2.462 2.558 0.318
ENSG00000077097 E016 212.6419786 0.0002227470 2.931898e-03 2.298937e-02 3 25615431 25615472 42 - 2.256 2.344 0.295
ENSG00000077097 E017 337.9686785 0.0011338697 7.757576e-03 4.815993e-02 3 25615473 25615586 114 - 2.458 2.536 0.263
ENSG00000077097 E018 0.6434498 0.0194979861 2.472966e-01   3 25615781 25615941 161 - 0.250 0.000 -9.622
ENSG00000077097 E019 313.4489147 0.0009816369 7.226667e-03 4.570445e-02 3 25618418 25618509 92 - 2.426 2.504 0.261
ENSG00000077097 E020 425.9720619 0.0063708377 3.396649e-01 5.777072e-01 3 25618654 25618849 196 - 2.565 2.619 0.180
ENSG00000077097 E021 403.8561159 0.0002242005 4.163072e-01 6.408497e-01 3 25619862 25620062 201 - 2.552 2.577 0.083
ENSG00000077097 E022 403.3394816 0.0019006289 2.677019e-01 5.073707e-01 3 25620682 25620816 135 - 2.566 2.538 -0.091
ENSG00000077097 E023 522.2661958 0.0001598453 3.579006e-01 5.940745e-01 3 25623515 25623746 232 - 2.672 2.664 -0.028
ENSG00000077097 E024 226.5617556 0.0006123142 3.895275e-01 6.200835e-01 3 25624297 25624351 55 - 2.300 2.331 0.103
ENSG00000077097 E025 288.0926576 0.0001783330 8.932792e-01 9.482939e-01 3 25624352 25624445 94 - 2.409 2.421 0.039
ENSG00000077097 E026 321.7572477 0.0001771725 2.366452e-02 1.080463e-01 3 25624682 25624803 122 - 2.472 2.429 -0.144
ENSG00000077097 E027 7.4493441 0.0386035519 5.227674e-01 7.168944e-01 3 25624804 25625038 235 - 0.906 0.802 -0.400
ENSG00000077097 E028 286.5999637 0.0002627915 3.962672e-06 9.425110e-05 3 25626560 25626674 115 - 2.437 2.330 -0.358
ENSG00000077097 E029 277.5178791 0.0002676782 4.012887e-12 3.725973e-10 3 25626772 25626864 93 - 2.438 2.265 -0.577
ENSG00000077097 E030 1.5304025 0.1401933248 7.935612e-01 8.924121e-01 3 25627057 25627186 130 - 0.370 0.415 0.247
ENSG00000077097 E031 305.0806628 0.0001917505 7.845817e-10 4.668536e-08 3 25627187 25627296 110 - 2.472 2.330 -0.472
ENSG00000077097 E032 316.6302795 0.0005296181 3.966145e-08 1.619618e-06 3 25628847 25628952 106 - 2.484 2.351 -0.444
ENSG00000077097 E033 310.7472435 0.0009535354 2.094287e-10 1.388747e-08 3 25629035 25629145 111 - 2.484 2.312 -0.575
ENSG00000077097 E034 301.2512570 0.0002631149 5.377000e-12 4.864882e-10 3 25630029 25630154 126 - 2.470 2.305 -0.551
ENSG00000077097 E035 1.0408063 0.0251692363 4.307552e-01   3 25630155 25630155 1 - 0.336 0.186 -1.131
ENSG00000077097 E036 329.8143775 0.0001657651 1.623560e-07 5.730986e-06 3 25630312 25630469 158 - 2.498 2.386 -0.375
ENSG00000077097 E037 293.2190835 0.0002431431 2.430768e-12 2.342794e-10 3 25630801 25630939 139 - 2.460 2.290 -0.565
ENSG00000077097 E038 220.9518517 0.0002777758 2.311743e-07 7.825138e-06 3 25632446 25632583 138 - 2.332 2.192 -0.468
ENSG00000077097 E039 237.6142642 0.0002254461 7.677332e-11 5.576508e-09 3 25632693 25632794 102 - 2.371 2.198 -0.576
ENSG00000077097 E040 321.4983333 0.0001876674 3.133625e-23 1.340457e-20 3 25633841 25634014 174 - 2.512 2.275 -0.791
ENSG00000077097 E041 407.1403283 0.0005702063 2.964273e-26 1.683618e-23 3 25635936 25636148 213 - 2.617 2.366 -0.838
ENSG00000077097 E042 262.5200406 0.0011738142 2.624980e-16 4.707307e-14 3 25637215 25637312 98 - 2.427 2.173 -0.849
ENSG00000077097 E043 210.9712680 0.0060182240 6.465876e-05 1.038251e-03 3 25638165 25638249 85 - 2.323 2.131 -0.642
ENSG00000077097 E044 184.9321276 0.0057184115 5.487692e-05 9.020775e-04 3 25638250 25638310 61 - 2.267 2.067 -0.667
ENSG00000077097 E045 211.9086054 0.0049504320 3.152417e-06 7.751997e-05 3 25642322 25642385 64 - 2.327 2.111 -0.723
ENSG00000077097 E046 256.5670631 0.0024097469 1.899470e-10 1.269975e-08 3 25643694 25643784 91 - 2.412 2.183 -0.767
ENSG00000077097 E047 221.9314370 0.0008642352 9.774505e-12 8.400485e-10 3 25645300 25645455 156 - 2.348 2.136 -0.707
ENSG00000077097 E048 10.3049011 0.0034699651 3.223277e-02 1.339106e-01 3 25645456 25645470 15 - 1.062 0.802 -0.981
ENSG00000077097 E049 61.5497191 0.0007694293 4.926905e-02 1.778438e-01 3 25664229 25664340 112 - 1.775 1.680 -0.318
ENSG00000077097 E050 101.9137515 0.0133472823 3.422247e-01 5.799481e-01 3 25664341 25664829 489 - 1.981 1.926 -0.185
ENSG00000077097 E051 62.1431693 0.0013754480 1.301497e-03 1.217697e-02 3 25664830 25664872 43 - 1.796 1.629 -0.568
ENSG00000077097 E052 48.0289948 0.0066461198 2.368989e-02 1.081362e-01 3 25664873 25664875 3 - 1.683 1.536 -0.503
ENSG00000077097 E053 45.2086713 0.0039179793 1.800433e-02 8.904272e-02 3 25664876 25665009 134 - 1.659 1.510 -0.509