ENSG00000076321

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000209884 ENSG00000076321 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL20 protein_coding protein_coding 5.497439 2.158024 9.203196 0.4555404 0.9910887 2.087321 3.5623237 1.3678159 5.5751995 0.15196932 0.4334241 2.019226 0.68797500 0.66616667 0.61736667 -0.04880000 0.941724716 0.008486845 FALSE  
ENST00000479505 ENSG00000076321 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL20 protein_coding processed_transcript 5.497439 2.158024 9.203196 0.4555404 0.9910887 2.087321 0.5155181 0.4020837 0.7901883 0.40208374 0.7901883 0.957402 0.06530000 0.13486667 0.07090000 -0.06396667 0.944017687 0.008486845 FALSE  
ENST00000483154 ENSG00000076321 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL20 protein_coding processed_transcript 5.497439 2.158024 9.203196 0.4555404 0.9910887 2.087321 0.5619166 0.0000000 0.7263720 0.00000000 0.2248736 6.202363 0.08720417 0.00000000 0.07940000 0.07940000 0.008486845 0.008486845 FALSE  
MSTRG.2605.1 ENSG00000076321 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL20 protein_coding   5.497439 2.158024 9.203196 0.4555404 0.9910887 2.087321 0.2799683 0.1865286 0.8108898 0.14384507 0.5165479 2.062450 0.06198333 0.09643333 0.09683333 0.00040000 1.000000000 0.008486845 FALSE  
MSTRG.2605.3 ENSG00000076321 HEK293_OSMI2_6hA HEK293_TMG_6hB KLHL20 protein_coding   5.497439 2.158024 9.203196 0.4555404 0.9910887 2.087321 0.5393783 0.1528374 1.2234709 0.06601486 0.3318171 2.921220 0.08395833 0.06793333 0.12856667 0.06063333 0.556998672 0.008486845 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000076321 E001 0.5963342 0.0235334803 0.86198995   1 173714941 173714953 13 + 0.177 0.209 0.290
ENSG00000076321 E002 4.9568015 0.0108567623 0.74924942 0.86630752 1 173714954 173714980 27 + 0.730 0.673 -0.236
ENSG00000076321 E003 17.4274282 0.0053093994 0.75693726 0.87081952 1 173714981 173715078 98 + 1.189 1.215 0.094
ENSG00000076321 E004 2.3416853 0.0252902553 0.01727732 0.08646195 1 173715303 173715522 220 + 0.547 0.000 -11.118
ENSG00000076321 E005 4.8535168 0.0821357088 0.62081662 0.78439483 1 173715523 173715566 44 + 0.725 0.614 -0.470
ENSG00000076321 E006 6.8584934 0.1138674844 0.99454624 0.99887332 1 173715567 173715654 88 + 0.821 0.825 0.016
ENSG00000076321 E007 22.9638553 0.0018078328 0.80530538 0.89933040 1 173716003 173716066 64 + 1.301 1.317 0.056
ENSG00000076321 E008 86.0233209 0.0006090005 0.50719710 0.70581750 1 173733713 173734286 574 + 1.853 1.876 0.079
ENSG00000076321 E009 0.3150090 0.0307659727 0.33396941   1 173734287 173734513 227 + 0.068 0.209 1.879
ENSG00000076321 E010 34.6764400 0.0018877035 0.71015615 0.84187785 1 173751764 173751922 159 + 1.481 1.450 -0.107
ENSG00000076321 E011 31.9702466 0.0017497049 0.84220146 0.92069973 1 173753213 173753307 95 + 1.449 1.430 -0.063
ENSG00000076321 E012 43.5857598 0.0009285811 0.25028184 0.48826666 1 173755923 173756038 116 + 1.556 1.621 0.220
ENSG00000076321 E013 55.3109182 0.0006917070 0.82736969 0.91226086 1 173756976 173757159 184 + 1.675 1.658 -0.057
ENSG00000076321 E014 52.0503306 0.0016754651 0.05668954 0.19528812 1 173766146 173766289 144 + 1.666 1.544 -0.414
ENSG00000076321 E015 51.5735217 0.0165764698 0.08421973 0.25319893 1 173774305 173774438 134 + 1.668 1.512 -0.533
ENSG00000076321 E016 58.7479042 0.0146818580 0.94318266 0.97392954 1 173775634 173775842 209 + 1.699 1.685 -0.049
ENSG00000076321 E017 0.1614157 0.0351292959 1.00000000   1 173781913 173782123 211 + 0.068 0.000 -7.454
ENSG00000076321 E018 36.2415437 0.0014023061 0.63483682 0.79346076 1 173782124 173782230 107 + 1.486 1.512 0.090
ENSG00000076321 E019 132.2420856 0.0017588410 0.05543393 0.19231781 1 173785163 173786692 1530 + 2.029 2.091 0.207