ENSG00000074935

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368657 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding retained_intron 18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 3.802153 2.7237708 5.904705 0.5105834 0.3138419 1.113414 0.20300417 0.32770000 0.18430000 -0.143400000 0.001329136 0.001329136 FALSE TRUE
ENST00000368662 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding protein_coding 18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 4.350367 1.8743796 7.084294 0.4581385 0.4831112 1.912569 0.25515833 0.23273333 0.22053333 -0.012200000 1.000000000 0.001329136 FALSE TRUE
ENST00000441191 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding processed_transcript 18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 1.830671 1.1118473 1.640369 0.6976991 0.7875628 0.556912 0.11868750 0.11286667 0.05096667 -0.061900000 0.947331952 0.001329136 FALSE FALSE
ENST00000604689 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding retained_intron 18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 1.268794 0.3294016 3.119031 0.0406179 0.2495230 3.204651 0.05767917 0.04266667 0.09726667 0.054600000 0.119002522 0.001329136 FALSE FALSE
ENST00000605457 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding nonsense_mediated_decay 18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 1.779913 0.6830279 2.321988 0.3918668 0.5892075 1.750575 0.09344167 0.07696667 0.07290000 -0.004066667 0.968041003 0.001329136 FALSE TRUE
MSTRG.28819.2 ENSG00000074935 HEK293_OSMI2_6hA HEK293_TMG_6hB TUBE1 protein_coding   18.71766 8.311659 32.06482 1.550609 0.61525 1.946498 3.800482 0.7829786 8.939993 0.3915114 0.4964248 3.496533 0.15711667 0.09730000 0.27853333 0.181233333 0.523691297 0.001329136 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000074935 E001 35.423420 0.0017032510 6.288045e-01 7.897223e-01 6 112070663 112071111 449 - 1.495 1.451 -0.153
ENSG00000074935 E002 23.028161 0.0348008289 4.753662e-01 6.837528e-01 6 112071112 112071264 153 - 1.290 1.352 0.216
ENSG00000074935 E003 43.322406 0.0061949530 3.486641e-01 5.861186e-01 6 112071265 112071328 64 - 1.592 1.510 -0.282
ENSG00000074935 E004 122.769873 0.0003395086 3.392735e-05 5.990481e-04 6 112071329 112071570 242 - 2.055 1.882 -0.584
ENSG00000074935 E005 115.960804 0.0005484384 5.526520e-04 6.153489e-03 6 112071902 112072076 175 - 2.029 1.878 -0.507
ENSG00000074935 E006 44.560548 0.0008646080 8.616059e-03 5.212329e-02 6 112072758 112072781 24 - 1.625 1.441 -0.628
ENSG00000074935 E007 80.367542 0.0009441192 9.521652e-05 1.435415e-03 6 112072782 112072898 117 - 1.882 1.673 -0.707
ENSG00000074935 E008 89.557592 0.0015503877 9.254404e-04 9.303027e-03 6 112074710 112074850 141 - 1.922 1.748 -0.588
ENSG00000074935 E009 5.624399 0.0050904667 6.950729e-03 4.440241e-02 6 112074851 112075084 234 - 0.833 0.339 -2.295
ENSG00000074935 E010 20.212098 0.0093037505 2.562391e-02 1.141444e-01 6 112075085 112075936 852 - 1.299 1.044 -0.911
ENSG00000074935 E011 63.306905 0.0006416593 5.329452e-04 5.975658e-03 6 112075937 112075998 62 - 1.775 1.569 -0.699
ENSG00000074935 E012 87.452630 0.0004902262 5.081583e-07 1.569321e-05 6 112075999 112076112 114 - 1.924 1.668 -0.866
ENSG00000074935 E013 14.150261 0.0029076389 8.262083e-02 2.499473e-01 6 112076113 112076171 59 - 1.147 0.939 -0.760
ENSG00000074935 E014 27.223147 0.0039308161 8.872967e-01 9.452331e-01 6 112076172 112076321 150 - 1.379 1.381 0.009
ENSG00000074935 E015 93.757483 0.0006235179 3.644903e-01 5.994464e-01 6 112076322 112076434 113 - 1.919 1.871 -0.161
ENSG00000074935 E016 93.080775 0.0023536135 1.466977e-01 3.574882e-01 6 112076435 112076509 75 - 1.890 1.949 0.197
ENSG00000074935 E017 102.943522 0.0255754134 4.287435e-04 5.013462e-03 6 112076510 112078694 2185 - 2.003 1.663 -1.145
ENSG00000074935 E018 17.662231 0.0020864631 7.888764e-05 1.226713e-03 6 112079400 112079632 233 - 1.274 0.801 -1.739
ENSG00000074935 E019 126.603156 0.0003555374 1.485074e-05 2.955928e-04 6 112079633 112079754 122 - 1.999 2.139 0.467
ENSG00000074935 E020 130.586298 0.0005393775 5.137821e-09 2.579131e-07 6 112081092 112081203 112 - 2.000 2.188 0.628
ENSG00000074935 E021 65.759246 0.0009295664 1.796112e-04 2.444471e-03 6 112081204 112081207 4 - 1.710 1.881 0.579
ENSG00000074935 E022 3.122106 0.0103565933 3.948403e-03 2.885612e-02 6 112083916 112084188 273 - 0.638 0.000 -12.331
ENSG00000074935 E023 78.408143 0.0022239386 7.714642e-07 2.267827e-05 6 112084189 112084246 58 - 1.768 1.990 0.748
ENSG00000074935 E024 3.617312 0.0086446081 2.032236e-02 9.701479e-02 6 112085334 112085654 321 - 0.672 0.202 -2.644
ENSG00000074935 E025 1.249338 0.0137882108 9.061408e-02   6 112085655 112085674 20 - 0.381 0.000 -11.080
ENSG00000074935 E026 3.373979 0.0139507182 3.613468e-01 5.968081e-01 6 112085675 112085743 69 - 0.620 0.444 -0.832
ENSG00000074935 E027 91.171382 0.0006442358 2.483986e-06 6.309273e-05 6 112086556 112086608 53 - 1.846 2.028 0.613
ENSG00000074935 E028 5.404525 0.1333601473 3.726228e-01 6.061354e-01 6 112086814 112087232 419 - 0.784 0.529 -1.094
ENSG00000074935 E029 80.554356 0.0007735382 6.554028e-09 3.197428e-07 6 112087233 112087306 74 - 1.775 2.014 0.802
ENSG00000074935 E030 54.136807 0.0009042913 5.512618e-08 2.177644e-06 6 112087410 112087529 120 - 1.591 1.861 0.912