ENSG00000069329

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000299138 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding protein_coding 40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 2.004582 0.4641716 3.147333 0.1003878 0.08169369 2.7352252 0.05604583 0.06203333 0.04630000 -0.015733333 6.974318e-01 1.656727e-10 FALSE TRUE
ENST00000568784 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding nonsense_mediated_decay 40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 1.379270 0.9152501 1.413837 0.3093062 0.42587270 0.6218687 0.06190000 0.11663333 0.02093333 -0.095700000 7.439315e-03 1.656727e-10 FALSE TRUE
ENST00000647959 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding nonsense_mediated_decay 40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 3.628854 1.0089998 5.558297 0.4484938 0.05065655 2.4500824 0.11883333 0.11916667 0.08173333 -0.037433333 8.777693e-01 1.656727e-10 FALSE TRUE
MSTRG.12564.3 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding   40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 8.961522 1.8217552 15.545628 0.1070446 0.28563704 3.0861380 0.24325417 0.26336667 0.22850000 -0.034866667 9.172843e-01 1.656727e-10 FALSE TRUE
MSTRG.12564.4 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding   40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 4.194111 0.8970349 6.954045 0.3176600 0.47146572 2.9406956 0.11748750 0.11096667 0.10160000 -0.009366667 9.579336e-01 1.656727e-10 FALSE TRUE
MSTRG.12564.6 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding   40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 15.027074 2.2332061 26.969183 0.3561396 2.03258348 3.5882126 0.31989583 0.30946667 0.39386667 0.084400000 5.228334e-01 1.656727e-10 FALSE TRUE
MSTRG.12564.8 ENSG00000069329 HEK293_OSMI2_6hA HEK293_TMG_6hB VPS35 protein_coding   40.40042 7.488636 68.28205 1.514221 2.947035 3.187018 4.097757 0.0000000 7.416825 0.0000000 1.15409279 9.5366019 0.05777083 0.00000000 0.10836667 0.108366667 1.656727e-10 1.656727e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000069329 E001 16.4003873 0.0181395799 1.795573e-02 8.889619e-02 16 46656132 46658076 1945 - 1.026 1.315 1.030
ENSG00000069329 E002 157.5909681 0.0313546881 2.376809e-01 4.738906e-01 16 46658077 46659012 936 - 2.002 2.157 0.520
ENSG00000069329 E003 73.3735694 0.0100326331 2.778802e-03 2.205264e-02 16 46659013 46659334 322 - 1.664 1.881 0.733
ENSG00000069329 E004 72.6009573 0.0005177767 2.300529e-04 3.001788e-03 16 46659335 46659676 342 - 1.661 1.869 0.705
ENSG00000069329 E005 312.5281926 0.0012854276 5.321843e-02 1.872873e-01 16 46659677 46660204 528 - 2.310 2.380 0.233
ENSG00000069329 E006 195.1246291 0.0003075442 8.211162e-04 8.452713e-03 16 46660205 46660335 131 - 2.097 2.222 0.420
ENSG00000069329 E007 399.0844874 0.0001780388 5.107508e-05 8.474572e-04 16 46660336 46660651 316 - 2.410 2.517 0.357
ENSG00000069329 E008 199.1918293 0.0002193934 2.111510e-02 9.971718e-02 16 46661718 46661826 109 - 2.113 2.202 0.296
ENSG00000069329 E009 142.7351571 0.0003493335 3.855523e-03 2.834076e-02 16 46661827 46661861 35 - 1.964 2.090 0.421
ENSG00000069329 E010 356.9899338 0.0013549556 8.433104e-02 2.534074e-01 16 46662243 46662482 240 - 2.369 2.429 0.201
ENSG00000069329 E011 320.7401706 0.0006582013 2.077718e-01 4.391286e-01 16 46662983 46663162 180 - 2.325 2.371 0.150
ENSG00000069329 E012 277.7049771 0.0012670927 4.254430e-02 1.613624e-01 16 46668930 46669052 123 - 2.259 2.334 0.250
ENSG00000069329 E013 0.9475002 0.0153787590 5.030748e-01   16 46669053 46669214 162 - 0.237 0.000 -11.272
ENSG00000069329 E014 313.4684585 0.0006926727 5.199055e-01 7.149493e-01 16 46671705 46671860 156 - 2.318 2.344 0.088
ENSG00000069329 E015 335.5436890 0.0001643842 2.403090e-02 1.091760e-01 16 46672265 46672417 153 - 2.356 2.296 -0.200
ENSG00000069329 E016 222.9922743 0.0002366784 1.129883e-01 3.041429e-01 16 46672418 46672472 55 - 2.179 2.128 -0.170
ENSG00000069329 E017 325.5206237 0.0002111503 3.685081e-02 1.465360e-01 16 46674314 46674462 149 - 2.345 2.289 -0.187
ENSG00000069329 E018 235.4675980 0.0004969678 6.299186e-01 7.903909e-01 16 46674564 46674660 97 - 2.196 2.220 0.078
ENSG00000069329 E019 0.0000000       16 46676505 46676577 73 -      
ENSG00000069329 E020 0.3336024 0.0244411696 6.288544e-03   16 46676578 46676582 5 - 0.000 0.435 13.535
ENSG00000069329 E021 218.7320433 0.0017195728 2.887131e-01 5.294150e-01 16 46676583 46676692 110 - 2.170 2.133 -0.123
ENSG00000069329 E022 234.8248059 0.0002362769 7.750888e-02 2.398730e-01 16 46677315 46677398 84 - 2.202 2.147 -0.186
ENSG00000069329 E023 5.0307088 0.0371172834 3.694069e-01 6.035577e-01 16 46677399 46677686 288 - 0.617 0.788 0.710
ENSG00000069329 E024 425.6186002 0.0001385539 5.513448e-05 9.058729e-04 16 46678943 46679156 214 - 2.464 2.363 -0.338
ENSG00000069329 E025 165.1027456 0.0002871101 3.859179e-04 4.595592e-03 16 46680671 46680674 4 - 2.060 1.908 -0.511
ENSG00000069329 E026 177.6488348 0.0002822023 2.358421e-04 3.066723e-03 16 46680675 46680686 12 - 2.091 1.939 -0.510
ENSG00000069329 E027 367.3177318 0.0001789508 5.607888e-04 6.229385e-03 16 46680687 46680853 167 - 2.400 2.307 -0.309
ENSG00000069329 E028 337.8522263 0.0023179244 4.355722e-03 3.113092e-02 16 46681377 46681500 124 - 2.365 2.263 -0.341
ENSG00000069329 E029 6.1466728 0.0095076387 1.238438e-02 6.797300e-02 16 46681501 46681520 20 - 0.642 1.019 1.477
ENSG00000069329 E030 0.9584589 0.0155902309 7.633482e-01   16 46681521 46681755 235 - 0.206 0.270 0.507
ENSG00000069329 E031 0.9475002 0.0153787590 5.030748e-01   16 46681756 46682078 323 - 0.237 0.000 -11.272
ENSG00000069329 E032 254.5935163 0.0006131071 1.282793e-05 2.603599e-04 16 46682079 46682175 97 - 2.247 2.090 -0.525
ENSG00000069329 E033 1.8433888 0.0151259669 7.395261e-01 8.601285e-01 16 46682478 46682642 165 - 0.345 0.270 -0.494
ENSG00000069329 E034 0.8545700 0.3902935129 8.759379e-01   16 46682643 46682666 24 - 0.210 0.000 -10.363
ENSG00000069329 E035 0.3559677 0.5261358924 1.000000e+00   16 46682667 46682684 18 - 0.097 0.000 -8.927
ENSG00000069329 E036 0.5255656 0.2761857929 3.307930e-01   16 46682882 46683507 626 - 0.094 0.273 1.852
ENSG00000069329 E037 188.0343022 0.0002682771 7.209570e-02 2.290133e-01 16 46683508 46683606 99 - 2.107 2.042 -0.217
ENSG00000069329 E038 0.8511734 0.0611684354 6.207214e-01   16 46683607 46683607 1 - 0.172 0.270 0.823
ENSG00000069329 E039 9.0634847 0.0356214148 1.330946e-09 7.592382e-08 16 46688314 46689130 817 - 0.604 1.433 3.114
ENSG00000069329 E040 77.2724892 0.0005001530 3.093999e-01 5.499028e-01 16 46689131 46689221 91 - 1.724 1.668 -0.189
ENSG00000069329 E041 7.3061329 0.0694670690 5.096358e-02 1.819456e-01 16 46689335 46689607 273 - 0.692 1.058 1.412