ENSG00000069011

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265340 ENSG00000069011 HEK293_OSMI2_6hA HEK293_TMG_6hB PITX1 protein_coding protein_coding 40.8426 68.0328 22.06464 15.17242 0.8911942 -1.624053 23.591993 42.050446 7.887559 11.9106220 0.27086128 -2.4129854 0.5278500 0.59423333 0.3595667 -0.23466667 7.121181e-02 2.696762e-05 FALSE TRUE
ENST00000504936 ENSG00000069011 HEK293_OSMI2_6hA HEK293_TMG_6hB PITX1 protein_coding retained_intron 40.8426 68.0328 22.06464 15.17242 0.8911942 -1.624053 2.747009 3.353138 2.466129 0.6575808 0.05406121 -0.4417217 0.0801875 0.06206667 0.1121000 0.05003333 4.959805e-01 2.696762e-05   FALSE
ENST00000506438 ENSG00000069011 HEK293_OSMI2_6hA HEK293_TMG_6hB PITX1 protein_coding protein_coding 40.8426 68.0328 22.06464 15.17242 0.8911942 -1.624053 7.510070 12.492339 7.357788 2.7983251 0.61880110 -0.7628946 0.2168542 0.18386667 0.3325000 0.14863333 2.696762e-05 2.696762e-05 FALSE TRUE
MSTRG.26964.2 ENSG00000069011 HEK293_OSMI2_6hA HEK293_TMG_6hB PITX1 protein_coding   40.8426 68.0328 22.06464 15.17242 0.8911942 -1.624053 4.902865 7.357375 2.236002 1.4758015 0.36479487 -1.7137911 0.1141542 0.11133333 0.1004667 -0.01086667 9.300236e-01 2.696762e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000069011 E001 1.679056 0.0116232405 1.097164e-01 2.985527e-01 5 135021946 135021976 31 - 0.593 0.300 -1.553
ENSG00000069011 E002 761.706695 0.0778899527 9.792500e-01 9.913932e-01 5 135027734 135028552 819 - 2.710 2.755 0.148
ENSG00000069011 E003 898.243262 0.0516216630 2.787188e-01 5.188111e-01 5 135028553 135029221 669 - 2.861 2.811 -0.168
ENSG00000069011 E004 341.425503 0.0002112871 1.616291e-01 3.791625e-01 5 135029222 135029286 65 - 2.434 2.406 -0.094
ENSG00000069011 E005 260.619034 0.0318824311 8.468590e-01 9.232293e-01 5 135029287 135029321 35 - 2.307 2.280 -0.091
ENSG00000069011 E006 213.070430 0.8900172093 5.407539e-01 7.295469e-01 5 135031276 135031285 10 - 2.186 2.198 0.041
ENSG00000069011 E007 307.823065 1.0464362395 5.560359e-01 7.405282e-01 5 135031286 135031335 50 - 2.342 2.358 0.051
ENSG00000069011 E008 397.769279 1.0964222717 4.768211e-01 6.848808e-01 5 135031336 135031508 173 - 2.393 2.479 0.285
ENSG00000069011 E009 42.425615 0.1448152928 1.865085e-02 9.123321e-02 5 135031509 135032010 502 - 1.834 1.401 -1.475
ENSG00000069011 E010 10.439990 0.0846998538 4.121539e-02 1.579056e-01 5 135032845 135033135 291 - 1.197 0.857 -1.251
ENSG00000069011 E011 25.549624 0.2571851251 7.398948e-01 8.603474e-01 5 135033438 135033712 275 - 1.474 1.252 -0.771
ENSG00000069011 E012 183.515527 0.4405935149 2.047008e-01 4.355435e-01 5 135033713 135033949 237 - 1.942 2.160 0.731
ENSG00000069011 E013 52.613240 0.0062446747 5.928341e-09 2.917846e-07 5 135033950 135034228 279 - 1.077 1.663 2.040
ENSG00000069011 E014 8.613002 0.0059941528 9.978137e-01 1.000000e+00 5 135034502 135034568 67 - 0.879 0.884 0.020
ENSG00000069011 E015 14.309137 0.0037620218 2.842910e-01 5.248092e-01 5 135034569 135034686 118 - 0.956 1.092 0.501
ENSG00000069011 E016 8.739051 0.0036755596 7.455918e-01 8.640495e-01 5 135034687 135034837 151 - 0.835 0.886 0.195