ENSG00000068654

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263857 ENSG00000068654 HEK293_OSMI2_6hA HEK293_TMG_6hB POLR1A protein_coding protein_coding 23.0955 31.01231 22.19088 3.370447 0.8147286 -0.4826891 11.851572 17.303041 8.329520 2.12519241 0.4394829 -1.0538229 0.5069500 0.5559667 0.3750000 -0.1809667 1.772219e-05 7.425905e-23 FALSE TRUE
ENST00000409681 ENSG00000068654 HEK293_OSMI2_6hA HEK293_TMG_6hB POLR1A protein_coding protein_coding 23.0955 31.01231 22.19088 3.370447 0.8147286 -0.4826891 3.615306 1.239665 6.952192 0.04740838 0.6336950 2.4779998 0.1652833 0.0408000 0.3120333 0.2712333 7.425905e-23 7.425905e-23 FALSE TRUE
MSTRG.18784.4 ENSG00000068654 HEK293_OSMI2_6hA HEK293_TMG_6hB POLR1A protein_coding   23.0955 31.01231 22.19088 3.370447 0.8147286 -0.4826891 6.625586 11.590318 5.802116 1.25706306 0.1956840 -0.9970289 0.2827292 0.3737667 0.2618667 -0.1119000 6.254804e-03 7.425905e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000068654 E001 17.3545399 0.0020626251 4.686570e-02 1.722214e-01 2 86020216 86020333 118 - 1.100 1.305 0.728
ENSG00000068654 E002 272.2863242 0.0006948751 2.656381e-10 1.721154e-08 2 86020334 86021272 939 - 2.280 2.476 0.654
ENSG00000068654 E003 206.6267788 0.0002194917 3.838352e-09 1.974676e-07 2 86021273 86021613 341 - 2.167 2.355 0.630
ENSG00000068654 E004 182.3971120 0.0002767083 1.801719e-05 3.496439e-04 2 86021614 86021942 329 - 2.139 2.291 0.506
ENSG00000068654 E005 340.4119738 0.0001711601 8.843304e-17 1.702717e-14 2 86021943 86022702 760 - 2.368 2.576 0.691
ENSG00000068654 E006 170.8636262 0.0007042619 3.832280e-07 1.223556e-05 2 86022703 86022994 292 - 2.088 2.277 0.634
ENSG00000068654 E007 780.5366700 0.0010454886 1.447744e-08 6.554523e-07 2 86022995 86025029 2035 - 2.776 2.920 0.478
ENSG00000068654 E008 759.0785428 0.0024188175 8.899313e-10 5.251244e-08 2 86025030 86026327 1298 - 2.725 2.921 0.653
ENSG00000068654 E009 835.0180571 0.0062048089 3.603526e-05 6.307583e-04 2 86026328 86027017 690 - 2.769 2.963 0.644
ENSG00000068654 E010 694.1769092 0.0031838747 6.780211e-08 2.622962e-06 2 86027018 86027523 506 - 2.688 2.883 0.650
ENSG00000068654 E011 229.8546248 0.0003173795 3.892899e-02 1.519814e-01 2 86027885 86027965 81 - 2.289 2.369 0.267
ENSG00000068654 E012 226.7856646 0.0003924503 1.435876e-02 7.572297e-02 2 86027966 86028049 84 - 2.277 2.368 0.304
ENSG00000068654 E013 273.9622656 0.0002325899 1.805048e-01 4.046312e-01 2 86028594 86028711 118 - 2.381 2.439 0.193
ENSG00000068654 E014 0.9417030 0.0158403988 1.175736e-01   2 86028712 86028799 88 - 0.434 0.172 -1.820
ENSG00000068654 E015 334.7311604 0.0002644584 2.187708e-01 4.519267e-01 2 86030196 86030396 201 - 2.506 2.507 0.003
ENSG00000068654 E016 203.0076748 0.0069617343 2.927659e-01 5.334724e-01 2 86031330 86031420 91 - 2.240 2.316 0.256
ENSG00000068654 E017 189.7865480 0.0030684160 1.195601e-01 3.151285e-01 2 86031421 86031503 83 - 2.204 2.290 0.286
ENSG00000068654 E018 108.0743546 0.0004317609 4.180970e-01 6.422100e-01 2 86031504 86031512 9 - 1.981 2.037 0.188
ENSG00000068654 E019 215.6463110 0.0040287602 8.901462e-01 9.467483e-01 2 86031513 86031635 123 - 2.295 2.327 0.106
ENSG00000068654 E020 165.7296616 0.0065093368 9.384483e-01 9.715083e-01 2 86032272 86032382 111 - 2.188 2.211 0.077
ENSG00000068654 E021 183.1378013 0.0022474323 8.176042e-02 2.483198e-01 2 86033661 86033787 127 - 2.265 2.230 -0.116
ENSG00000068654 E022 0.7844689 0.0224529833 4.811908e-01   2 86036978 86037070 93 - 0.155 0.294 1.174
ENSG00000068654 E023 261.0705551 0.0006801364 4.302818e-03 3.085409e-02 2 86038700 86038857 158 - 2.426 2.380 -0.154
ENSG00000068654 E024 0.8259751 0.2113390303 5.519350e-01   2 86038858 86038917 60 - 0.156 0.295 1.170
ENSG00000068654 E025 0.1308682 0.0310394913 8.836594e-01   2 86039325 86039326 2 - 0.000 0.094 8.716
ENSG00000068654 E026 122.5097876 0.0003835705 2.340282e-02 1.072406e-01 2 86039327 86039341 15 - 2.105 2.056 -0.165
ENSG00000068654 E027 228.3507691 0.0002371732 7.754254e-03 4.814811e-02 2 86039342 86039462 121 - 2.366 2.327 -0.130
ENSG00000068654 E028 2.0724661 0.0516687016 4.205528e-02 1.600689e-01 2 86039998 86040200 203 - 0.648 0.297 -1.814
ENSG00000068654 E029 227.7684775 0.0002687737 1.054796e-04 1.564442e-03 2 86040392 86040559 168 - 2.383 2.314 -0.231
ENSG00000068654 E030 260.7328036 0.0002034112 1.185707e-02 6.584924e-02 2 86041889 86042103 215 - 2.418 2.386 -0.105
ENSG00000068654 E031 267.2282316 0.0002091743 5.337253e-02 1.876153e-01 2 86042974 86043195 222 - 2.421 2.403 -0.058
ENSG00000068654 E032 209.7081058 0.0004940131 9.696375e-01 9.866437e-01 2 86044139 86044304 166 - 2.285 2.311 0.086
ENSG00000068654 E033 153.3025825 0.0014914707 1.803598e-01 4.044585e-01 2 86045278 86045360 83 - 2.178 2.158 -0.065
ENSG00000068654 E034 206.4118666 0.0003870225 1.914755e-01 4.187522e-01 2 86045617 86045769 153 - 2.301 2.292 -0.028
ENSG00000068654 E035 180.4669319 0.0002976392 1.167166e-02 6.511763e-02 2 86047165 86047263 99 - 2.264 2.220 -0.146
ENSG00000068654 E036 221.3661978 0.0002068989 3.480182e-05 6.124508e-04 2 86048884 86049042 159 - 2.375 2.298 -0.254
ENSG00000068654 E037 165.4530498 0.0007563576 3.138975e-05 5.615403e-04 2 86049160 86049242 83 - 2.264 2.163 -0.337
ENSG00000068654 E038 1.3108423 0.0136968632 1.166382e-01   2 86052329 86052816 488 - 0.498 0.238 -1.558
ENSG00000068654 E039 228.6299732 0.0003054306 2.744774e-04 3.472332e-03 2 86052817 86053000 184 - 2.381 2.316 -0.215
ENSG00000068654 E040 1.0955861 0.1737196605 3.749577e-01   2 86054138 86054139 2 - 0.154 0.354 1.565
ENSG00000068654 E041 204.3735710 0.0002638587 4.779053e-08 1.913683e-06 2 86054140 86054289 150 - 2.363 2.248 -0.383
ENSG00000068654 E042 221.0234459 0.0010073383 5.428217e-06 1.238361e-04 2 86065274 86065465 192 - 2.391 2.289 -0.341
ENSG00000068654 E043 0.3337900 0.0316458840 6.474553e-01   2 86065466 86065714 249 - 0.155 0.094 -0.821
ENSG00000068654 E044 242.0787598 0.0022510292 9.964366e-04 9.887108e-03 2 86070018 86070272 255 - 2.417 2.337 -0.266
ENSG00000068654 E045 0.1723744 0.0361606121 8.828534e-01   2 86074261 86075029 769 - 0.000 0.094 8.721
ENSG00000068654 E046 217.9030934 0.0018210160 3.531299e-05 6.199047e-04 2 86075030 86075260 231 - 2.384 2.280 -0.349
ENSG00000068654 E047 178.7324968 0.0008678296 5.918611e-11 4.391072e-09 2 86077859 86077981 123 - 2.334 2.165 -0.565
ENSG00000068654 E048 0.8086220 0.0931517139 5.748518e-02   2 86078041 86078113 73 - 0.432 0.095 -2.803
ENSG00000068654 E049 224.1513419 0.0002508713 1.866669e-18 4.548110e-16 2 86078114 86078284 171 - 2.444 2.255 -0.629
ENSG00000068654 E050 1.5865740 0.3303754954 1.107512e-01 3.002235e-01 2 86079773 86080815 1043 - 0.612 0.228 -2.162
ENSG00000068654 E051 200.7823634 0.0002589079 2.456783e-17 5.051069e-15 2 86080816 86080978 163 - 2.400 2.208 -0.639
ENSG00000068654 E052 77.8143191 0.0027101397 6.529050e-04 7.032301e-03 2 86081601 86081616 16 - 1.961 1.829 -0.443
ENSG00000068654 E053 104.3332553 0.0003725088 3.527201e-06 8.542496e-05 2 86081617 86081706 90 - 2.086 1.948 -0.465
ENSG00000068654 E054 0.8038701 0.0171085931 8.041911e-01   2 86081707 86083081 1375 - 0.269 0.237 -0.239
ENSG00000068654 E055 107.8503441 0.0003831783 9.983625e-07 2.837097e-05 2 86083082 86083168 87 - 2.104 1.959 -0.486
ENSG00000068654 E056 8.8360756 0.0839793888 1.527131e-02 7.909181e-02 2 86086254 86088565 2312 - 1.173 0.793 -1.415
ENSG00000068654 E057 136.7360096 0.0003425106 1.156387e-06 3.221475e-05 2 86088566 86088669 104 - 2.196 2.070 -0.422
ENSG00000068654 E058 111.0420054 0.0005216457 9.628882e-06 2.028539e-04 2 86088785 86088870 86 - 2.111 1.983 -0.431
ENSG00000068654 E059 0.5227326 0.3903927633 1.000000e+00   2 86089634 86089821 188 - 0.157 0.174 0.183
ENSG00000068654 E060 127.5643283 0.0003415124 1.171434e-08 5.396661e-07 2 86089822 86089929 108 - 2.184 2.027 -0.528
ENSG00000068654 E061 163.1392075 0.0003513140 8.947355e-11 6.412382e-09 2 86098611 86098760 150 - 2.293 2.132 -0.537
ENSG00000068654 E062 176.3356822 0.0014578125 2.399301e-09 1.300116e-07 2 86099968 86100172 205 - 2.330 2.163 -0.556
ENSG00000068654 E063 124.0116149 0.0021314886 4.707466e-08 1.889473e-06 2 86105700 86106155 456 - 2.190 2.005 -0.619