ENSG00000066855

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262146 ENSG00000066855 HEK293_OSMI2_6hA HEK293_TMG_6hB MTFR1 protein_coding protein_coding 19.79411 6.76578 32.22839 0.7067663 0.09658242 2.250321 15.9011621 4.4216594 26.205321 0.6181924 0.7336589 2.5644913 0.77912917 0.6483667 0.8129667 0.16460000 0.02286396 0.02286396 FALSE TRUE
ENST00000518352 ENSG00000066855 HEK293_OSMI2_6hA HEK293_TMG_6hB MTFR1 protein_coding retained_intron 19.79411 6.76578 32.22839 0.7067663 0.09658242 2.250321 0.4191981 0.5892810 1.015401 0.1221432 0.4440596 0.7748834 0.02761667 0.0932000 0.0316000 -0.06160000 0.39141732 0.02286396   FALSE
MSTRG.31582.1 ENSG00000066855 HEK293_OSMI2_6hA HEK293_TMG_6hB MTFR1 protein_coding   19.79411 6.76578 32.22839 0.7067663 0.09658242 2.250321 1.1536056 0.7917147 1.297025 0.1668567 0.4098431 0.7051256 0.06146667 0.1144333 0.0402000 -0.07423333 0.10203256 0.02286396 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000066855 E001 2.7733955 0.0083448849 0.668733790 0.81537088 8 65644719 65644720 2 + 0.492 0.575 0.389
ENSG00000066855 E002 7.0122381 0.0116312003 0.938774942 0.97167519 8 65644721 65644733 13 + 0.818 0.813 -0.017
ENSG00000066855 E003 7.3625963 0.0085294483 0.872796012 0.93748355 8 65644734 65644738 5 + 0.828 0.857 0.116
ENSG00000066855 E004 55.7204601 0.0008702830 0.137879328 0.34426349 8 65644739 65644784 46 + 1.625 1.717 0.313
ENSG00000066855 E005 2.8958211 0.0269088259 0.070896968 0.22650618 8 65662465 65662576 112 + 0.448 0.764 1.416
ENSG00000066855 E006 5.5844999 0.0053725332 0.019708307 0.09492082 8 65662577 65662724 148 + 0.635 0.968 1.324
ENSG00000066855 E007 1.6394708 0.1584452814 0.569064053 0.74955530 8 65662725 65662764 40 + 0.374 0.229 -0.979
ENSG00000066855 E008 0.3337900 0.0271092417 0.271027150   8 65662765 65662808 44 + 0.061 0.228 2.197
ENSG00000066855 E009 0.2922838 0.0257020385 0.272782437   8 65669871 65669872 2 + 0.061 0.228 2.194
ENSG00000066855 E010 104.6822101 0.0007348530 0.077747545 0.24028384 8 65669873 65669980 108 + 1.896 1.978 0.276
ENSG00000066855 E011 91.1393590 0.0005377777 0.839018058 0.91884472 8 65669981 65670018 38 + 1.850 1.866 0.055
ENSG00000066855 E012 0.0000000       8 65679503 65679650 148 +      
ENSG00000066855 E013 0.0000000       8 65682044 65682080 37 +      
ENSG00000066855 E014 0.2852693 0.2766236162 1.000000000   8 65682328 65682352 25 + 0.113 0.000 -8.149
ENSG00000066855 E015 123.6916828 0.0003952482 0.037727994 0.14890423 8 65682353 65682451 99 + 1.999 1.920 -0.267
ENSG00000066855 E016 0.4820342 0.0213473700 0.717871673   8 65689566 65689599 34 + 0.162 0.000 -9.370
ENSG00000066855 E017 142.4914802 0.0002973124 0.109561336 0.29834027 8 65693644 65693759 116 + 2.056 2.002 -0.181
ENSG00000066855 E018 141.3716536 0.0004779574 0.047051982 0.17265612 8 65704694 65704929 236 + 2.057 1.987 -0.237
ENSG00000066855 E019 25.8197107 0.0017145738 0.471705980 0.68133232 8 65707010 65707044 35 + 1.310 1.377 0.231
ENSG00000066855 E020 15.2667739 0.0140344550 0.742242051 0.86179340 8 65707045 65707052 8 + 1.116 1.080 -0.127
ENSG00000066855 E021 45.0130575 0.0010008438 0.778226726 0.88363679 8 65707053 65707129 77 + 1.550 1.575 0.085
ENSG00000066855 E022 99.2415202 0.0004259668 0.688837668 0.82833286 8 65707130 65707220 91 + 1.894 1.882 -0.038
ENSG00000066855 E023 100.1573199 0.0004032600 0.249614468 0.48744949 8 65707221 65707256 36 + 1.904 1.858 -0.153
ENSG00000066855 E024 94.7669976 0.0005883544 0.167113357 0.38671279 8 65707843 65707856 14 + 1.883 1.824 -0.199
ENSG00000066855 E025 105.8789204 0.0015979986 0.275505466 0.51557809 8 65707857 65707889 33 + 1.930 1.886 -0.149
ENSG00000066855 E026 149.9796504 0.0004526669 0.401984363 0.63002416 8 65707890 65708011 122 + 2.064 2.100 0.123
ENSG00000066855 E027 1.3475629 0.3311345465 0.825662298   8 65708012 65708338 327 + 0.318 0.230 -0.631
ENSG00000066855 E028 541.0175201 0.0048104384 0.948441740 0.97649196 8 65708976 65710562 1587 + 2.622 2.627 0.018
ENSG00000066855 E029 11.5674824 0.5059637987 0.072337089 0.22945387 8 65719380 65719481 102 + 0.824 1.391 2.060
ENSG00000066855 E030 4.7077683 0.0056014017 0.004321631 0.03094821 8 65719482 65719748 267 + 0.552 0.968 1.695
ENSG00000066855 E031 3.4667871 0.0790227818 0.442994654 0.66074709 8 65720404 65720596 193 + 0.536 0.704 0.735
ENSG00000066855 E032 2.7206703 0.0312365518 0.148428806 0.36003153 8 65725316 65725350 35 + 0.447 0.709 1.196
ENSG00000066855 E033 5.0683747 0.0448222288 0.158461545 0.37448393 8 65725351 65725513 163 + 0.648 0.893 0.986
ENSG00000066855 E034 3.8994065 0.0086574222 0.083796615 0.25236508 8 65725514 65725661 148 + 0.533 0.814 1.194
ENSG00000066855 E035 2.2908586 0.0294477859 0.363922438 0.59896833 8 65728361 65728603 243 + 0.400 0.576 0.871
ENSG00000066855 E036 1.2260943 0.0182016556 0.023529759   8 65770947 65771261 315 + 0.162 0.576 2.612