ENSG00000066777

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262215 ENSG00000066777 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF1 protein_coding protein_coding 6.68801 4.305744 9.830025 0.7902735 0.4631406 1.189053 3.4235900 1.78020674 5.60315541 0.45182184 0.06828338 1.648686 0.47421667 0.40280000 0.572566667 0.16976667 2.975827e-01 1.0489e-09 FALSE TRUE
ENST00000517955 ENSG00000066777 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF1 protein_coding retained_intron 6.68801 4.305744 9.830025 0.7902735 0.4631406 1.189053 0.4936228 0.69026020 0.00000000 0.58445261 0.00000000 -6.129819 0.12931667 0.14180000 0.000000000 -0.14180000 2.897122e-01 1.0489e-09 FALSE FALSE
ENST00000522878 ENSG00000066777 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF1 protein_coding processed_transcript 6.68801 4.305744 9.830025 0.7902735 0.4631406 1.189053 0.3872671 1.14133669 0.08725882 0.36626284 0.08725882 -3.565337 0.08905417 0.27706667 0.008133333 -0.26893333 7.233061e-03 1.0489e-09 FALSE FALSE
MSTRG.31630.5 ENSG00000066777 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF1 protein_coding   6.68801 4.305744 9.830025 0.7902735 0.4631406 1.189053 1.1575006 0.01347617 2.18425145 0.01347617 0.28384006 6.546388 0.12106250 0.00280000 0.222600000 0.21980000 1.048900e-09 1.0489e-09 FALSE TRUE
MSTRG.31630.6 ENSG00000066777 HEK293_OSMI2_6hA HEK293_TMG_6hB ARFGEF1 protein_coding   6.68801 4.305744 9.830025 0.7902735 0.4631406 1.189053 0.4830257 0.08704430 1.00671594 0.04754971 0.20709735 3.389129 0.05535417 0.02586667 0.101233333 0.07536667 2.768642e-01 1.0489e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000066777 E001 13.5776570 0.0319888939 3.018785e-03 2.351755e-02 8 67173511 67175486 1976 - 0.986 1.347 1.289
ENSG00000066777 E002 2.1208693 0.0103645663 9.623148e-02 2.751427e-01 8 67175487 67175572 86 - 0.356 0.647 1.435
ENSG00000066777 E003 1.2865538 0.1042249337 8.215840e-05   8 67197658 67197673 16 - 0.000 0.708 14.877
ENSG00000066777 E004 91.1191879 0.0011664680 2.360682e-11 1.906315e-09 8 67197674 67198404 731 - 1.812 2.095 0.953
ENSG00000066777 E005 131.7521315 0.0088365669 2.741410e-04 3.468837e-03 8 67198405 67199010 606 - 1.995 2.208 0.714
ENSG00000066777 E006 49.6600200 0.0089281941 5.534776e-03 3.739089e-02 8 67199011 67199098 88 - 1.578 1.780 0.684
ENSG00000066777 E007 4.8754584 0.1737504484 3.008495e-01 5.413233e-01 8 67199099 67199928 830 - 0.634 0.877 0.982
ENSG00000066777 E008 3.9162619 0.0876720735 3.183212e-02 1.327034e-01 8 67199929 67200395 467 - 0.503 0.899 1.668
ENSG00000066777 E009 44.8401202 0.0011070184 6.151887e-03 4.046839e-02 8 67200396 67200513 118 - 1.553 1.720 0.570
ENSG00000066777 E010 54.6257110 0.0018305164 1.511833e-04 2.113120e-03 8 67201467 67201605 139 - 1.626 1.838 0.721
ENSG00000066777 E011 0.3447487 0.7108199162 1.166153e-01   8 67201606 67201889 284 - 0.000 0.337 13.068
ENSG00000066777 E012 67.2718417 0.0012746060 3.893520e-04 4.628048e-03 8 67203083 67203251 169 - 1.722 1.903 0.608
ENSG00000066777 E013 46.0499386 0.0008244299 4.538545e-05 7.660763e-04 8 67204680 67204766 87 - 1.539 1.774 0.797
ENSG00000066777 E014 32.3798466 0.0024448541 1.508386e-03 1.367200e-02 8 67204767 67204819 53 - 1.391 1.614 0.766
ENSG00000066777 E015 0.9223018 0.0431344635 1.901457e-01   8 67207095 67207190 96 - 0.189 0.434 1.659
ENSG00000066777 E016 40.0295643 0.0010076977 5.061437e-02 1.811177e-01 8 67211483 67211615 133 - 1.514 1.643 0.441
ENSG00000066777 E017 20.8885148 0.0018714439 1.028366e-02 5.933082e-02 8 67216590 67216607 18 - 1.221 1.437 0.753
ENSG00000066777 E018 30.5633387 0.0144682422 4.658347e-02 1.715184e-01 8 67216608 67216662 55 - 1.387 1.562 0.602
ENSG00000066777 E019 45.6533958 0.0010902329 6.916496e-02 2.231048e-01 8 67217782 67217920 139 - 1.576 1.691 0.390
ENSG00000066777 E020 43.8752172 0.0009109877 7.686290e-03 4.788543e-02 8 67218003 67218138 136 - 1.542 1.706 0.559
ENSG00000066777 E021 43.1763368 0.0039908540 1.488101e-02 7.763628e-02 8 67219431 67219560 130 - 1.532 1.697 0.560
ENSG00000066777 E022 44.7459260 0.0011319675 8.116065e-01 9.032252e-01 8 67224903 67225033 131 - 1.589 1.614 0.085
ENSG00000066777 E023 52.5526069 0.0070166299 7.565408e-01 8.705653e-01 8 67226023 67226183 161 - 1.668 1.655 -0.045
ENSG00000066777 E024 53.1594960 0.0006709070 1.903420e-01 4.173950e-01 8 67227137 67227309 173 - 1.683 1.620 -0.216
ENSG00000066777 E025 55.9086529 0.0008776461 2.673668e-01 5.069902e-01 8 67227447 67227598 152 - 1.704 1.654 -0.171
ENSG00000066777 E026 49.2105890 0.0011832864 4.907201e-01 6.944865e-01 8 67227963 67228132 170 - 1.648 1.619 -0.097
ENSG00000066777 E027 21.4981499 0.0052865806 9.225536e-02 2.679233e-01 8 67228224 67228264 41 - 1.329 1.184 -0.511
ENSG00000066777 E028 42.9329843 0.0076529495 8.230926e-01 9.097803e-01 8 67232855 67232945 91 - 1.578 1.569 -0.032
ENSG00000066777 E029 56.7109053 0.0009061682 8.233621e-01 9.098801e-01 8 67238343 67238493 151 - 1.689 1.711 0.076
ENSG00000066777 E030 49.5032942 0.0010162346 5.026489e-01 7.026029e-01 8 67238735 67238893 159 - 1.628 1.676 0.164
ENSG00000066777 E031 33.6686900 0.0011478699 1.734618e-01 3.951733e-01 8 67240162 67240290 129 - 1.454 1.555 0.347
ENSG00000066777 E032 40.5958496 0.0013367918 1.528458e-01 3.663927e-01 8 67251299 67251450 152 - 1.532 1.631 0.336
ENSG00000066777 E033 53.9206397 0.0007028489 2.657167e-01 5.051303e-01 8 67253451 67253622 172 - 1.688 1.637 -0.174
ENSG00000066777 E034 41.1814120 0.0009870783 1.172276e-02 6.532763e-02 8 67257732 67257816 85 - 1.595 1.437 -0.542
ENSG00000066777 E035 53.3045813 0.0008529024 3.154202e-03 2.433030e-02 8 67258085 67258290 206 - 1.706 1.541 -0.559
ENSG00000066777 E036 33.2241451 0.0012605662 7.857992e-02 2.419418e-01 8 67259815 67259926 112 - 1.496 1.379 -0.406
ENSG00000066777 E037 46.0059412 0.0117336296 8.890604e-03 5.332635e-02 8 67266006 67266207 202 - 1.653 1.451 -0.692
ENSG00000066777 E038 37.5656563 0.0009387837 9.302722e-06 1.971320e-04 8 67266876 67266984 109 - 1.583 1.260 -1.117
ENSG00000066777 E039 40.1365007 0.0011984525 2.325470e-05 4.344058e-04 8 67267091 67267230 140 - 1.607 1.311 -1.020
ENSG00000066777 E040 26.3737459 0.0018239353 1.570330e-05 3.103434e-04 8 67267343 67267442 100 - 1.443 1.053 -1.374
ENSG00000066777 E041 46.3231506 0.0009758393 6.463334e-04 6.973461e-03 8 67271702 67271936 235 - 1.656 1.446 -0.721
ENSG00000066777 E042 41.9630189 0.0011064123 2.237758e-04 2.934539e-03 8 67275976 67276109 134 - 1.622 1.378 -0.836
ENSG00000066777 E043 56.8796595 0.0006729113 2.816007e-07 9.323898e-06 8 67277282 67277457 176 - 1.757 1.455 -1.031
ENSG00000066777 E044 44.2726255 0.0032078103 4.186838e-05 7.153133e-04 8 67287955 67288065 111 - 1.646 1.357 -0.994
ENSG00000066777 E045 63.6695003 0.0015245397 3.385125e-07 1.098342e-05 8 67291847 67292123 277 - 1.805 1.512 -0.996
ENSG00000066777 E046 39.3420764 0.0037916886 5.863496e-06 1.323616e-04 8 67296431 67296610 180 - 1.610 1.259 -1.210
ENSG00000066777 E047 27.2189049 0.0013190029 2.547772e-04 3.271856e-03 8 67299209 67299313 105 - 1.443 1.134 -1.084
ENSG00000066777 E048 14.9261789 0.0033716886 3.341736e-03 2.543263e-02 8 67299314 67299355 42 - 1.197 0.864 -1.224
ENSG00000066777 E049 27.7868335 0.0119990649 8.486187e-04 8.679534e-03 8 67301224 67301380 157 - 1.455 1.116 -1.188
ENSG00000066777 E050 15.5174123 0.0885650907 2.160591e-02 1.013138e-01 8 67302436 67302466 31 - 1.226 0.795 -1.594
ENSG00000066777 E051 21.0533148 0.0017103634 4.501633e-04 5.224004e-03 8 67343164 67343336 173 - 1.344 1.007 -1.202
ENSG00000066777 E052 6.4796934 0.0093679060 5.485892e-02 1.910253e-01 8 67343337 67343509 173 - 0.878 0.586 -1.195
ENSG00000066777 E053 10.2564294 0.0438927860 1.080106e-01 2.957259e-01 8 67343510 67343787 278 - 1.044 0.787 -0.975