ENSG00000065911

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000394053 ENSG00000065911 HEK293_OSMI2_6hA HEK293_TMG_6hB MTHFD2 protein_coding protein_coding 87.96807 22.85563 156.572 3.605494 11.26927 2.775666 29.125234 9.700158 47.710381 2.0768525 1.0972387 2.297039 0.3668375 0.41490000 0.3071667 -0.10773333 0.27424131 0.02614027 FALSE TRUE
ENST00000462026 ENSG00000065911 HEK293_OSMI2_6hA HEK293_TMG_6hB MTHFD2 protein_coding protein_coding 87.96807 22.85563 156.572 3.605494 11.26927 2.775666 47.100725 10.153724 90.610722 1.3054928 8.3629863 3.156412 0.4910042 0.44943333 0.5770667 0.12763333 0.02614027 0.02614027 FALSE FALSE
ENST00000470592 ENSG00000065911 HEK293_OSMI2_6hA HEK293_TMG_6hB MTHFD2 protein_coding nonsense_mediated_decay 87.96807 22.85563 156.572 3.605494 11.26927 2.775666 5.187764 1.546890 7.996594 0.3811601 0.6864165 2.362522 0.0671250 0.06566667 0.0510000 -0.01466667 0.67577679 0.02614027 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000065911 E001 0.000000       2 74186172 74186212 41 +      
ENSG00000065911 E002 0.000000       2 74186213 74186464 252 +      
ENSG00000065911 E003 0.000000       2 74186465 74187120 656 +      
ENSG00000065911 E004 0.000000       2 74187121 74187382 262 +      
ENSG00000065911 E005 0.000000       2 74187383 74187457 75 +      
ENSG00000065911 E006 8.862139 0.0782612060 8.758000e-01 0.939147731 2 74198610 74198612 3 + 0.892 0.897 0.022
ENSG00000065911 E007 9.040122 0.0883132704 8.467647e-01 0.923174442 2 74198613 74198613 1 + 0.901 0.897 -0.014
ENSG00000065911 E008 9.170991 0.0888402148 9.796302e-01 0.991627517 2 74198614 74198614 1 + 0.901 0.932 0.117
ENSG00000065911 E009 9.653025 0.0852209802 8.745451e-01 0.938483731 2 74198615 74198615 1 + 0.925 0.932 0.027
ENSG00000065911 E010 9.653025 0.0852209802 8.745451e-01 0.938483731 2 74198616 74198616 1 + 0.925 0.932 0.027
ENSG00000065911 E011 128.175333 0.0258944441 1.650952e-01 0.384074107 2 74198617 74198628 12 + 2.012 1.937 -0.250
ENSG00000065911 E012 320.952472 0.0188128254 5.384787e-01 0.727958947 2 74198629 74198742 114 + 2.393 2.400 0.023
ENSG00000065911 E013 21.645792 0.0018290632 6.030273e-01 0.772271000 2 74201382 74201399 18 + 1.248 1.231 -0.060
ENSG00000065911 E014 35.464362 0.0083615153 9.762895e-01 0.989918023 2 74201400 74201479 80 + 1.446 1.482 0.126
ENSG00000065911 E015 11.610295 0.0985484903 1.390386e-01 0.346043809 2 74201480 74205704 4225 + 0.924 1.217 1.063
ENSG00000065911 E016 636.470013 0.0115059794 4.997434e-02 0.179605157 2 74205705 74205889 185 + 2.700 2.642 -0.194
ENSG00000065911 E017 4.733412 0.0968479550 6.035490e-01 0.772549869 2 74205890 74205981 92 + 0.645 0.769 0.514
ENSG00000065911 E018 5.112795 0.0384372458 9.917907e-01 0.997618191 2 74205982 74206088 107 + 0.699 0.714 0.062
ENSG00000065911 E019 2.945887 0.0087766466 7.268320e-01 0.852323174 2 74206384 74206393 10 + 0.490 0.578 0.414
ENSG00000065911 E020 3.296245 0.0437220926 5.367167e-01 0.726848802 2 74206394 74206413 20 + 0.512 0.653 0.636
ENSG00000065911 E021 4.096309 0.0254333680 7.401165e-01 0.860499114 2 74206414 74206606 193 + 0.617 0.580 -0.167
ENSG00000065911 E022 744.443785 0.0111556297 4.468462e-03 0.031764234 2 74207704 74207826 123 + 2.775 2.673 -0.339
ENSG00000065911 E023 818.173519 0.0072423935 7.581677e-04 0.007926757 2 74208569 74208721 153 + 2.815 2.718 -0.324
ENSG00000065911 E024 11.837765 0.0390426229 9.484049e-03 0.055890956 2 74209019 74209941 923 + 0.911 1.282 1.345
ENSG00000065911 E025 664.582839 0.0002424024 9.875976e-05 0.001479245 2 74209942 74210049 108 + 2.719 2.683 -0.118
ENSG00000065911 E026 523.992194 0.0242265016 3.957395e-01 0.625023683 2 74211199 74211291 93 + 2.593 2.664 0.239
ENSG00000065911 E027 554.925689 0.1411734676 3.013788e-01 0.541867885 2 74211741 74211866 126 + 2.607 2.728 0.404
ENSG00000065911 E028 5.133376 0.0220792637 1.021465e-04 0.001522720 2 74211970 74211996 27 + 0.529 1.108 2.314
ENSG00000065911 E029 7.387104 0.0995258521 2.034397e-02 0.097101565 2 74212075 74212109 35 + 0.721 1.132 1.559
ENSG00000065911 E030 5.365677 0.0071284469 2.866225e-04 0.003595707 2 74212110 74212128 19 + 0.567 1.084 2.052
ENSG00000065911 E031 2493.099770 0.0052159834 1.771283e-04 0.002415720 2 74214079 74217565 3487 + 3.258 3.381 0.411