ENSG00000065526

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375759 ENSG00000065526 HEK293_OSMI2_6hA HEK293_TMG_6hB SPEN protein_coding protein_coding 38.70618 62.23036 29.65222 6.446127 1.024498 -1.069224 5.290809 4.279168 9.4687864 0.8212618 0.61977175 1.1440047 0.1722542 0.07046667 0.31920000 0.2487333 5.052938e-06 1.077403e-13 FALSE TRUE
ENST00000487496 ENSG00000065526 HEK293_OSMI2_6hA HEK293_TMG_6hB SPEN protein_coding processed_transcript 38.70618 62.23036 29.65222 6.446127 1.024498 -1.069224 8.944246 12.490864 0.9990959 1.9824972 0.08008599 -3.6308926 0.1947250 0.19943333 0.03356667 -0.1658667 1.077403e-13 1.077403e-13 FALSE TRUE
MSTRG.376.5 ENSG00000065526 HEK293_OSMI2_6hA HEK293_TMG_6hB SPEN protein_coding   38.70618 62.23036 29.65222 6.446127 1.024498 -1.069224 12.306297 27.967099 6.6273470 4.5217651 0.56568746 -2.0755678 0.2903708 0.44340000 0.22486667 -0.2185333 4.364664e-03 1.077403e-13 FALSE TRUE
MSTRG.376.6 ENSG00000065526 HEK293_OSMI2_6hA HEK293_TMG_6hB SPEN protein_coding   38.70618 62.23036 29.65222 6.446127 1.024498 -1.069224 1.794046 1.482648 2.8706563 0.7418009 0.10844245 0.9485234 0.0518750 0.02533333 0.09713333 0.0718000 5.416586e-01 1.077403e-13 FALSE TRUE
MSTRG.376.9 ENSG00000065526 HEK293_OSMI2_6hA HEK293_TMG_6hB SPEN protein_coding   38.70618 62.23036 29.65222 6.446127 1.024498 -1.069224 8.845388 14.770246 8.6625134 1.2621535 0.33876290 -0.7691482 0.2443917 0.24176667 0.29206667 0.0503000 7.008834e-01 1.077403e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000065526 E001 12.4207130 0.1857222913 2.739862e-01 5.139451e-01 1 15836095 15836885 791 + 0.933 1.151 0.796
ENSG00000065526 E002 2.0463425 0.0212726155 5.085415e-01 7.068515e-01 1 15847707 15847799 93 + 0.389 0.498 0.568
ENSG00000065526 E003 2.1967995 0.0099512160 7.466751e-01 8.647508e-01 1 15847800 15847819 20 + 0.534 0.467 -0.323
ENSG00000065526 E004 4.6501737 0.0081529425 1.907089e-01 4.178019e-01 1 15847820 15847900 81 + 0.863 0.667 -0.786
ENSG00000065526 E005 3.9832586 0.0068709608 3.873112e-01 6.183845e-01 1 15847901 15847927 27 + 0.766 0.626 -0.587
ENSG00000065526 E006 10.8426255 0.0065427013 1.634353e-01 3.818490e-01 1 15847928 15847984 57 + 0.941 1.085 0.530
ENSG00000065526 E007 47.7036718 0.2056922152 8.400012e-01 9.193834e-01 1 15847985 15848150 166 + 1.759 1.597 -0.550
ENSG00000065526 E008 1.7016386 0.3471302814 2.914419e-01 5.320570e-01 1 15867879 15867884 6 + 0.172 0.476 2.034
ENSG00000065526 E009 2.1357492 0.2124783380 1.204368e-01 3.165405e-01 1 15867885 15867976 92 + 0.172 0.556 2.420
ENSG00000065526 E010 204.6999172 0.0059047686 1.146066e-15 1.906252e-13 1 15872816 15873136 321 + 2.505 2.126 -1.264
ENSG00000065526 E011 214.4293217 0.0069636705 8.503697e-03 5.162008e-02 1 15873502 15874395 894 + 2.393 2.250 -0.478
ENSG00000065526 E012 282.8078535 0.0093857798 1.401244e-08 6.359259e-07 1 15876202 15876575 374 + 2.609 2.298 -1.039
ENSG00000065526 E013 114.4426058 0.0051066936 5.458715e-07 1.673615e-05 1 15876576 15876678 103 + 2.191 1.931 -0.870
ENSG00000065526 E014 0.4929928 0.0209744949 2.187866e-01   1 15876679 15876711 33 + 0.294 0.085 -2.171
ENSG00000065526 E015 57.9362213 0.0044135063 2.083155e-05 3.954920e-04 1 15909321 15909323 3 + 1.897 1.638 -0.876
ENSG00000065526 E016 164.3556146 0.0068617433 1.682399e-08 7.512756e-07 1 15909324 15909481 158 + 2.367 2.071 -0.990
ENSG00000065526 E017 222.2928115 0.0072239429 4.132779e-11 3.171446e-09 1 15911101 15911301 201 + 2.517 2.184 -1.110
ENSG00000065526 E018 216.8377235 0.0097881550 2.269338e-09 1.236400e-07 1 15916128 15916279 152 + 2.510 2.170 -1.133
ENSG00000065526 E019 196.9520750 0.0076669129 5.145656e-10 3.164979e-08 1 15918926 15919051 126 + 2.462 2.134 -1.093
ENSG00000065526 E020 208.4116891 0.0032092232 4.782507e-20 1.429894e-17 1 15919404 15919517 114 + 2.501 2.146 -1.186
ENSG00000065526 E021 187.0252148 0.0024968237 9.621045e-22 3.461599e-19 1 15920870 15920966 97 + 2.456 2.100 -1.189
ENSG00000065526 E022 132.6649172 0.0032453180 3.596646e-15 5.613908e-13 1 15920967 15920983 17 + 2.301 1.960 -1.141
ENSG00000065526 E023 215.8636407 0.0052959432 2.792008e-14 3.845476e-12 1 15922249 15922349 101 + 2.512 2.165 -1.157
ENSG00000065526 E024 4567.8358664 0.0015288752 8.053365e-18 1.792460e-15 1 15928091 15932491 4401 + 3.721 3.572 -0.495
ENSG00000065526 E025 763.8534786 0.0001209941 1.746250e-02 8.716007e-02 1 15932492 15932972 481 + 2.838 2.855 0.058
ENSG00000065526 E026 4202.2616758 0.0005868587 8.853432e-17 1.702737e-14 1 15932973 15936266 3294 + 3.539 3.607 0.226
ENSG00000065526 E027 1027.4363759 0.0001777713 7.767996e-62 4.885868e-58 1 15937163 15937645 483 + 2.819 3.033 0.713
ENSG00000065526 E028 719.9350402 0.0017218824 1.427049e-13 1.718765e-11 1 15937812 15938006 195 + 2.700 2.866 0.554
ENSG00000065526 E029 52.8203190 0.0739034165 5.125159e-01 7.098649e-01 1 15938583 15938717 135 + 1.619 1.721 0.348
ENSG00000065526 E030 621.8394314 0.0005434590 2.241051e-32 2.212686e-29 1 15938718 15938876 159 + 2.598 2.816 0.725
ENSG00000065526 E031 2361.8010425 0.0035872520 4.226790e-22 1.584561e-19 1 15939296 15941451 2156 + 3.150 3.398 0.825