ENSG00000064961

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000333651 ENSG00000064961 HEK293_OSMI2_6hA HEK293_TMG_6hB HMG20B protein_coding protein_coding 129.6896 183.5498 89.31311 24.98303 0.6165734 -1.039144 87.131469 126.97719 37.260407 22.444016 4.6450006 -1.7685805 0.63013333 0.68393333 0.41763333 -0.26630000 0.0332928 5.605019e-07 FALSE TRUE
ENST00000416526 ENSG00000064961 HEK293_OSMI2_6hA HEK293_TMG_6hB HMG20B protein_coding protein_coding 129.6896 183.5498 89.31311 24.98303 0.6165734 -1.039144 7.175459 15.80952 11.168260 4.484123 2.0519421 -0.5010098 0.06692083 0.08696667 0.12500000 0.03803333 0.7311514 5.605019e-07 FALSE FALSE
ENST00000488973 ENSG00000064961 HEK293_OSMI2_6hA HEK293_TMG_6hB HMG20B protein_coding retained_intron 129.6896 183.5498 89.31311 24.98303 0.6165734 -1.039144 10.775855 13.65660 9.500596 2.081293 0.7262924 -0.5230471 0.09187500 0.07870000 0.10640000 0.02770000 0.7129817 5.605019e-07 FALSE TRUE
MSTRG.16178.15 ENSG00000064961 HEK293_OSMI2_6hA HEK293_TMG_6hB HMG20B protein_coding   129.6896 183.5498 89.31311 24.98303 0.6165734 -1.039144 1.908319 0.00000 8.190537 0.000000 4.2121364 9.6795746 0.01821667 0.00000000 0.09113333 0.09113333 0.2106450 5.605019e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000064961 E001 0.9114452 0.0921829579 2.033322e-01   19 3572777 3572799 23 + 0.000 0.297 10.827
ENSG00000064961 E002 1.0423134 0.0194221894 1.339707e-01   19 3572800 3572822 23 + 0.000 0.334 13.563
ENSG00000064961 E003 1.0423134 0.0194221894 1.339707e-01   19 3572823 3572824 2 + 0.000 0.334 13.563
ENSG00000064961 E004 1.2028192 0.0160500293 4.251557e-01   19 3572825 3572925 101 + 0.418 0.293 -0.749
ENSG00000064961 E005 3.4404417 0.1981901601 4.920562e-01 6.953803e-01 19 3572926 3572943 18 + 0.417 0.676 1.214
ENSG00000064961 E006 4.5832634 0.0912504484 1.391893e-01 3.463066e-01 19 3572944 3572947 4 + 0.418 0.789 1.673
ENSG00000064961 E007 42.3268793 0.2131045617 4.795667e-02 1.748984e-01 19 3572948 3572976 29 + 1.225 1.660 1.501
ENSG00000064961 E008 58.0678159 0.3676382994 1.421035e-01 3.505681e-01 19 3572977 3572978 2 + 1.422 1.784 1.235
ENSG00000064961 E009 105.9589535 0.5050076578 1.081006e-01 2.958912e-01 19 3572979 3572985 7 + 1.594 2.053 1.550
ENSG00000064961 E010 125.1011355 0.5397203521 9.800309e-02 2.782675e-01 19 3572986 3572994 9 + 1.637 2.128 1.653
ENSG00000064961 E011 90.2126787 0.0028959169 4.425836e-07 1.391810e-05 19 3572995 3573291 297 + 1.656 1.973 1.070
ENSG00000064961 E012 313.3878312 0.1259639095 2.903662e-03 2.282171e-02 19 3573292 3573347 56 + 2.019 2.526 1.693
ENSG00000064961 E013 249.4720185 1.0026569938 1.769283e-01 3.996502e-01 19 3573692 3573696 5 + 1.898 2.429 1.779
ENSG00000064961 E014 325.6575166 1.1486955677 2.346240e-01 4.704211e-01 19 3573697 3573744 48 + 2.059 2.539 1.606
ENSG00000064961 E015 420.3660951 1.2540875905 3.243916e-01 5.636816e-01 19 3573745 3573800 56 + 2.258 2.638 1.268
ENSG00000064961 E016 91.0809360 0.1211684676 6.513481e-01 8.044076e-01 19 3573801 3573902 102 + 1.930 1.901 -0.099
ENSG00000064961 E017 240.3976541 0.0743908990 1.068777e-01 2.938225e-01 19 3573903 3574292 390 + 2.439 2.278 -0.537
ENSG00000064961 E018 59.1563484 0.0602214514 5.674186e-02 1.953905e-01 19 3574293 3574340 48 + 1.861 1.664 -0.667
ENSG00000064961 E019 71.4939660 0.0421267594 7.204404e-03 4.560605e-02 19 3574341 3574369 29 + 1.975 1.724 -0.846
ENSG00000064961 E020 75.2839316 0.0217184280 2.869682e-03 2.262374e-02 19 3574370 3574382 13 + 1.974 1.760 -0.720
ENSG00000064961 E021 422.5970080 0.0142488979 1.643220e-01 3.831564e-01 19 3574383 3574409 27 + 2.489 2.596 0.357
ENSG00000064961 E022 1100.4945599 0.0001033252 3.860295e-01 6.173237e-01 19 3574410 3574586 177 + 2.954 3.003 0.163
ENSG00000064961 E023 28.3824617 0.0854519668 2.902414e-03 2.281612e-02 19 3575502 3575539 38 + 1.671 1.269 -1.382
ENSG00000064961 E024 551.3213990 0.0002754242 8.229233e-02 2.492796e-01 19 3575540 3575541 2 + 2.685 2.691 0.020
ENSG00000064961 E025 1128.3684836 0.0005225129 9.101522e-05 1.381399e-03 19 3575542 3575650 109 + 3.016 2.992 -0.081
ENSG00000064961 E026 551.2035250 0.0002280649 8.222146e-10 4.880495e-08 19 3575651 3575660 10 + 2.740 2.670 -0.234
ENSG00000064961 E027 23.2309407 0.1606550766 7.042845e-03 4.483234e-02 19 3575661 3575875 215 + 1.616 1.157 -1.593
ENSG00000064961 E028 52.7106231 0.0825616872 1.233657e-04 1.779372e-03 19 3575876 3576260 385 + 1.986 1.476 -1.727
ENSG00000064961 E029 489.0371571 0.0007374424 3.743816e-08 1.539433e-06 19 3576261 3576263 3 + 2.695 2.613 -0.274
ENSG00000064961 E030 776.3408268 0.0018875445 2.745371e-04 3.472827e-03 19 3576264 3576307 44 + 2.874 2.819 -0.183
ENSG00000064961 E031 30.3265081 0.1236699869 3.051776e-04 3.782104e-03 19 3576308 3576351 44 + 1.786 1.204 -2.002
ENSG00000064961 E032 55.8108795 0.1193700092 7.640638e-04 7.978753e-03 19 3576352 3576552 201 + 2.009 1.500 -1.724
ENSG00000064961 E033 896.5890658 0.0040472817 7.377566e-02 2.323250e-01 19 3576553 3576615 63 + 2.915 2.889 -0.084
ENSG00000064961 E034 600.9054460 0.0043336389 2.003348e-01 4.301559e-01 19 3576616 3576625 10 + 2.731 2.719 -0.041
ENSG00000064961 E035 6.1059319 0.0046498352 1.074868e-03 1.049125e-02 19 3576885 3576891 7 + 1.051 0.669 -1.482
ENSG00000064961 E036 1188.5561613 0.0031030296 2.466444e-01 4.843350e-01 19 3576892 3577107 216 + 2.968 3.035 0.222
ENSG00000064961 E037 727.0020712 0.0017972984 6.591035e-03 4.265529e-02 19 3577981 3578113 133 + 2.728 2.831 0.343
ENSG00000064961 E038 28.3835586 0.0205671555 2.658235e-01 5.052485e-01 19 3578114 3578444 331 + 1.470 1.391 -0.272
ENSG00000064961 E039 881.9411712 0.0008112919 6.195917e-01 7.835349e-01 19 3578509 3578701 193 + 2.872 2.899 0.091
ENSG00000064961 E040 1140.3580547 0.0040758090 1.506392e-01 3.632085e-01 19 3578702 3579088 387 + 3.008 2.998 -0.033