ENSG00000063854

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397353 ENSG00000063854 HEK293_OSMI2_6hA HEK293_TMG_6hB HAGH protein_coding protein_coding 74.69565 109.5854 34.93242 15.82024 0.8153102 -1.649136 12.587525 18.42275 3.802471 2.658994 1.9358260 -2.2734733 0.15608333 0.1681 0.10883333 -0.05926667 0.85412072 0.03370096 FALSE TRUE
ENST00000564445 ENSG00000063854 HEK293_OSMI2_6hA HEK293_TMG_6hB HAGH protein_coding protein_coding 74.69565 109.5854 34.93242 15.82024 0.8153102 -1.649136 27.834904 31.41485 20.526314 3.671309 2.8176965 -0.6137286 0.39828333 0.2892 0.58646667 0.29726667 0.03370096 0.03370096 FALSE TRUE
MSTRG.11800.10 ENSG00000063854 HEK293_OSMI2_6hA HEK293_TMG_6hB HAGH protein_coding   74.69565 109.5854 34.93242 15.82024 0.8153102 -1.649136 7.095617 10.71910 2.243853 1.906066 0.4615218 -2.2510636 0.08956667 0.0968 0.06376667 -0.03303333 0.48744461 0.03370096 FALSE TRUE
MSTRG.11800.3 ENSG00000063854 HEK293_OSMI2_6hA HEK293_TMG_6hB HAGH protein_coding   74.69565 109.5854 34.93242 15.82024 0.8153102 -1.649136 16.675871 32.83563 2.612720 6.253930 1.3137457 -3.6465660 0.20780417 0.2953 0.07530000 -0.22000000 0.54704537 0.03370096 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000063854 E001 0.5171231 0.9576836336 1.000000e+00   16 1795620 1795720 101 - 0.000 0.170 8.802
ENSG00000063854 E002 1.0944886 0.0152481716 5.263196e-01   16 1806803 1806880 78 - 0.371 0.248 -0.815
ENSG00000063854 E003 3.2205691 0.0074694709 6.838325e-01 8.251745e-01 16 1807577 1807628 52 - 0.482 0.558 0.362
ENSG00000063854 E004 9.2976098 0.0036117346 2.076004e-01 4.389303e-01 16 1807629 1807764 136 - 0.759 0.939 0.698
ENSG00000063854 E005 12.0698286 0.0035345364 1.241591e-02 6.811082e-02 16 1807765 1807948 184 - 0.704 1.059 1.364
ENSG00000063854 E006 30.1923440 0.0012163106 3.416975e-01 5.794741e-01 16 1807949 1808869 921 - 1.329 1.407 0.274
ENSG00000063854 E007 2.9457812 0.0084125323 3.506347e-01 5.878004e-01 16 1808870 1808871 2 - 0.372 0.557 0.944
ENSG00000063854 E008 11.4348706 0.0315987507 5.746953e-01 7.534605e-01 16 1808872 1809025 154 - 0.929 1.010 0.300
ENSG00000063854 E009 8.7316122 0.1040297263 7.980796e-01 8.952170e-01 16 1809026 1809102 77 - 0.857 0.898 0.156
ENSG00000063854 E010 7.0181345 0.1666823492 4.038450e-01 6.313940e-01 16 1809103 1809104 2 - 0.649 0.839 0.774
ENSG00000063854 E011 7.4999810 0.0887085647 6.391829e-01 7.963549e-01 16 1809105 1809106 2 - 0.763 0.852 0.350
ENSG00000063854 E012 26.0654049 0.0336345302 5.080212e-01 7.064549e-01 16 1809107 1809123 17 - 1.255 1.347 0.323
ENSG00000063854 E013 153.2077191 0.0003384428 4.484470e-02 1.672302e-01 16 1809124 1809237 114 - 2.022 2.098 0.254
ENSG00000063854 E014 488.1567183 0.0001976227 7.819059e-02 2.411836e-01 16 1809238 1809382 145 - 2.554 2.590 0.120
ENSG00000063854 E015 39.6595451 0.0010769690 1.645008e-01 3.833785e-01 16 1809383 1809446 64 - 1.416 1.518 0.352
ENSG00000063854 E016 522.7432955 0.0019846491 9.070087e-01 9.556099e-01 16 1809754 1809833 80 - 2.610 2.608 -0.005
ENSG00000063854 E017 3.6541976 0.1261662396 5.089201e-01 7.070800e-01 16 1809834 1810887 1054 - 0.701 0.557 -0.627
ENSG00000063854 E018 0.1723744 0.0392766052 1.000000e+00   16 1812359 1812419 61 - 0.000 0.062 9.569
ENSG00000063854 E019 616.4383619 0.0002907996 7.114001e-01 8.427007e-01 16 1816893 1816994 102 - 2.678 2.683 0.017
ENSG00000063854 E020 362.4035456 0.0001874794 5.962445e-01 7.678243e-01 16 1817168 1817188 21 - 2.464 2.451 -0.044
ENSG00000063854 E021 554.9595498 0.0001234282 3.675413e-01 6.020271e-01 16 1817189 1817271 83 - 2.652 2.635 -0.058
ENSG00000063854 E022 19.4771540 0.1087874044 6.337698e-02 2.103068e-01 16 1818515 1819114 600 - 1.445 1.128 -1.114
ENSG00000063854 E023 523.2709865 0.0002085000 1.481547e-01 3.596918e-01 16 1819115 1819223 109 - 2.636 2.606 -0.098
ENSG00000063854 E024 1.5058740 0.0125461875 8.994107e-01 9.515720e-01 16 1819835 1819835 1 - 0.372 0.348 -0.142
ENSG00000063854 E025 3.4506604 0.0242250992 5.387852e-01 7.281402e-01 16 1819836 1819896 61 - 0.482 0.606 0.580
ENSG00000063854 E026 645.8297241 0.0001122010 5.038003e-03 3.483985e-02 16 1819897 1820014 118 - 2.742 2.695 -0.159
ENSG00000063854 E027 34.1138765 0.0264066190 1.682234e-03 1.489634e-02 16 1821512 1822299 788 - 1.680 1.357 -1.109
ENSG00000063854 E028 488.3868253 0.0001727788 4.181431e-02 1.594555e-01 16 1822300 1822364 65 - 2.618 2.577 -0.137
ENSG00000063854 E029 583.7385797 0.0001643962 8.906705e-01 9.470133e-01 16 1822865 1823037 173 - 2.659 2.661 0.004
ENSG00000063854 E030 46.5593125 0.0203581479 7.286738e-01 8.534062e-01 16 1826507 1826602 96 - 1.550 1.568 0.063
ENSG00000063854 E031 4.3193118 0.0101931820 9.864014e-01 9.950520e-01 16 1826603 1826711 109 - 0.642 0.641 -0.007
ENSG00000063854 E032 193.7625846 0.0005532602 2.742564e-14 3.786589e-12 16 1826712 1826940 229 - 1.933 2.226 0.984
ENSG00000063854 E033 16.2413587 0.0165846474 8.594448e-01 9.300611e-01 16 1826941 1828997 2057 - 1.119 1.134 0.057