ENSG00000060982

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261192 ENSG00000060982 HEK293_OSMI2_6hA HEK293_TMG_6hB BCAT1 protein_coding protein_coding 13.17357 5.968822 14.42388 1.203728 0.4566242 1.271526 6.009328 1.5968668 8.924202 0.5866226 0.29552357 2.475088 0.3930417 0.2443667 0.6186333 0.37426667 1.324559e-02 1.103849e-23 FALSE TRUE
ENST00000355164 ENSG00000060982 HEK293_OSMI2_6hA HEK293_TMG_6hB BCAT1 protein_coding processed_transcript 13.17357 5.968822 14.42388 1.203728 0.4566242 1.271526 2.937319 3.5684614 1.541042 0.6602090 0.06255957 -1.206101 0.3297833 0.6045000 0.1072333 -0.49726667 1.103849e-23 1.103849e-23 FALSE FALSE
ENST00000539780 ENSG00000060982 HEK293_OSMI2_6hA HEK293_TMG_6hB BCAT1 protein_coding protein_coding 13.17357 5.968822 14.42388 1.203728 0.4566242 1.271526 2.181577 0.5790407 2.112744 0.0209273 0.36053401 1.849491 0.1443833 0.1062333 0.1452000 0.03896667 7.485633e-01 1.103849e-23 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000060982 E001 606.5565830 0.0186445755 6.698944e-01 8.162227e-01 12 24810024 24814802 4779 - 2.658 2.731 0.241
ENSG00000060982 E002 9.5143207 0.0182191861 7.491987e-01 8.662868e-01 12 24814803 24814820 18 - 0.919 0.897 -0.082
ENSG00000060982 E003 200.4567645 0.0013297531 4.115435e-14 5.507452e-12 12 24814821 24816334 1514 - 2.233 1.972 -0.872
ENSG00000060982 E004 47.0465764 0.0008658602 2.507947e-03 2.037930e-02 12 24816335 24816541 207 - 1.604 1.423 -0.621
ENSG00000060982 E005 123.4703095 0.0215245179 5.229705e-03 3.582735e-02 12 24816542 24817479 938 - 2.014 1.839 -0.589
ENSG00000060982 E006 21.1728650 0.0020452858 5.355659e-02 1.880055e-01 12 24817480 24817480 1 - 1.268 1.104 -0.586
ENSG00000060982 E007 40.3352603 0.0291170258 4.560266e-02 1.690340e-01 12 24817481 24817655 175 - 1.537 1.356 -0.622
ENSG00000060982 E008 39.6605132 0.0014822157 2.338619e-01 4.695990e-01 12 24817656 24817808 153 - 1.509 1.455 -0.186
ENSG00000060982 E009 69.7407530 0.0005310752 8.591283e-01 9.298841e-01 12 24817809 24818049 241 - 1.734 1.775 0.137
ENSG00000060982 E010 43.9757612 0.0008948785 9.572173e-02 2.742996e-01 12 24829823 24829897 75 - 1.519 1.654 0.459
ENSG00000060982 E011 0.0000000       12 24830230 24830328 99 -      
ENSG00000060982 E012 57.3696344 0.0266296253 6.379121e-01 7.956764e-01 12 24832723 24832863 141 - 1.650 1.697 0.162
ENSG00000060982 E013 0.0000000       12 24833811 24834807 997 -      
ENSG00000060982 E014 0.4764247 0.0244156374 5.260331e-01   12 24836447 24836510 64 - 0.115 0.228 1.189
ENSG00000060982 E015 39.1398889 0.2597834678 3.561747e-01 5.926821e-01 12 24836511 24836596 86 - 1.469 1.598 0.441
ENSG00000060982 E016 52.0600040 0.0331892749 3.699721e-01 6.039267e-01 12 24842082 24842224 143 - 1.599 1.694 0.323
ENSG00000060982 E017 63.1519247 0.0006566126 7.669427e-01 8.767984e-01 12 24849786 24849949 164 - 1.691 1.738 0.160
ENSG00000060982 E018 28.0915071 0.0012426385 1.949443e-01 4.232805e-01 12 24878530 24878535 6 - 1.372 1.292 -0.278
ENSG00000060982 E019 48.1609621 0.0007826012 9.243401e-01 9.645052e-01 12 24878536 24878649 114 - 1.575 1.612 0.126
ENSG00000060982 E020 42.4120100 0.0069984544 8.069151e-01 9.002637e-01 12 24881301 24881411 111 - 1.527 1.534 0.021
ENSG00000060982 E021 58.9351918 0.0006796689 2.585372e-01 4.973556e-01 12 24894275 24894475 201 - 1.676 1.640 -0.120
ENSG00000060982 E022 36.1707218 0.0058108238 7.054681e-01 8.390097e-01 12 24901814 24901885 72 - 1.463 1.464 0.006
ENSG00000060982 E023 0.2852693 0.3763777915 1.000000e+00   12 24901963 24902388 426 - 0.113 0.000 -9.687
ENSG00000060982 E024 0.0000000       12 24902947 24903075 129 -      
ENSG00000060982 E025 27.2694686 0.0300613342 5.367931e-22 1.990389e-19 12 24945431 24945816 386 - 0.903 1.929 3.585
ENSG00000060982 E026 49.0605194 0.0011075329 2.877496e-09 1.522321e-07 12 24948927 24949131 205 - 1.503 1.861 1.214