ENSG00000055732

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341115 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding protein_coding 5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 0.4156215 0.04909709 0.3556020 0.04909709 0.28867744 2.6291147 0.05601250 0.008866667 0.06546667 0.0566 8.301824e-01 3.814654e-07 FALSE TRUE
ENST00000370587 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding protein_coding 5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 0.4258829 0.42062406 0.5075158 0.24332873 0.13472850 0.2651740 0.08706250 0.127933333 0.09723333 -0.0307 8.598261e-01 3.814654e-07 FALSE TRUE
ENST00000370589 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding protein_coding 5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 1.7897933 0.42233772 2.4886999 0.09758021 0.11740941 2.5309470 0.30670417 0.202133333 0.47313333 0.2710 2.108332e-01 3.814654e-07 FALSE TRUE
ENST00000474447 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding processed_transcript 5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 1.4269266 1.64717201 0.9374648 0.81240558 0.02527062 -0.8065791 0.34901667 0.534866667 0.17786667 -0.3570 3.814654e-07 3.814654e-07 FALSE TRUE
ENST00000490600 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding nonsense_mediated_decay 5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 0.3572832 0.24994356 0.3671071 0.13160731 0.09555183 0.5367758 0.05987917 0.087200000 0.06900000 -0.0182 9.834472e-01 3.814654e-07 FALSE TRUE
MSTRG.1514.2 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding   5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 0.4176631 0.00000000 0.0000000 0.00000000 0.00000000 0.0000000 0.07858333 0.000000000 0.00000000 0.0000   3.814654e-07 FALSE TRUE
MSTRG.1514.4 ENSG00000055732 HEK293_OSMI2_6hA HEK293_TMG_6hB MCOLN3 protein_coding   5.152586 2.94361 5.271218 1.2975 0.1071206 0.8383912 0.1542139 0.00000000 0.3991213 0.00000000 0.05909771 5.3544566 0.02608333 0.000000000 0.07620000 0.0762 4.637075e-04 3.814654e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000055732 E001 0.7007164 0.151561060 7.284979e-01   1 85018082 85018082 1 - 0.182 0.259 0.642
ENSG00000055732 E002 58.6620826 0.000870785 1.210205e-01 3.174898e-01 1 85018083 85018907 825 - 1.727 1.799 0.241
ENSG00000055732 E003 5.2230562 0.021245956 7.529132e-01 8.684919e-01 1 85018908 85018908 1 - 0.791 0.737 -0.217
ENSG00000055732 E004 40.5499487 0.001309611 8.851943e-01 9.440534e-01 1 85018909 85019257 349 - 1.602 1.596 -0.019
ENSG00000055732 E005 27.5027347 0.010848203 9.222719e-01 9.634226e-01 1 85021070 85021276 207 - 1.434 1.440 0.021
ENSG00000055732 E006 27.4473075 0.001525546 9.220024e-01 9.632853e-01 1 85022070 85022192 123 - 1.436 1.442 0.019
ENSG00000055732 E007 25.7965832 0.010329788 5.394268e-01 7.285611e-01 1 85022299 85022400 102 - 1.403 1.447 0.154
ENSG00000055732 E008 41.4537352 0.006065332 5.352997e-15 8.182377e-13 1 85022401 85023520 1120 - 1.345 1.852 1.729
ENSG00000055732 E009 4.4908392 0.007119818 4.457327e-02 1.665508e-01 1 85024541 85024556 16 - 0.584 0.873 1.189
ENSG00000055732 E010 13.2880398 0.023197909 1.663040e-01 3.856511e-01 1 85024557 85024823 267 - 1.045 1.250 0.731
ENSG00000055732 E011 31.4526564 0.002148127 5.376795e-04 6.015944e-03 1 85024824 85025938 1115 - 1.394 1.617 0.768
ENSG00000055732 E012 19.7320954 0.002242263 5.816841e-03 3.883146e-02 1 85025939 85026088 150 - 1.384 1.156 -0.800
ENSG00000055732 E013 4.9986919 0.006015280 9.305770e-01 9.672987e-01 1 85026089 85026171 83 - 0.752 0.767 0.063
ENSG00000055732 E014 19.1586039 0.001873739 3.975214e-03 2.901780e-02 1 85026172 85026284 113 - 1.370 1.131 -0.841
ENSG00000055732 E015 24.2672222 0.001984725 3.460055e-02 1.404995e-01 1 85029106 85029205 100 - 1.441 1.283 -0.548
ENSG00000055732 E016 4.9183833 0.007496960 4.453029e-01 6.623110e-01 1 85029206 85029959 754 - 0.710 0.823 0.455
ENSG00000055732 E017 22.5657010 0.001841586 1.908119e-02 9.275584e-02 1 85032696 85032792 97 - 1.424 1.244 -0.626
ENSG00000055732 E018 18.9646587 0.002069213 2.010693e-05 3.836220e-04 1 85032872 85032956 85 - 1.402 1.029 -1.323
ENSG00000055732 E019 21.0452455 0.016245632 3.027203e-04 3.757829e-03 1 85034098 85034251 154 - 1.448 1.063 -1.356
ENSG00000055732 E020 2.3510284 0.009786212 6.556882e-01 8.071372e-01 1 85036710 85036868 159 - 0.554 0.480 -0.354
ENSG00000055732 E021 15.8387167 0.048849358 1.482474e-02 7.740962e-02 1 85041010 85041177 168 - 1.322 0.967 -1.275
ENSG00000055732 E022 16.3881800 0.023881317 1.562033e-01 3.712185e-01 1 85045133 85045362 230 - 1.288 1.133 -0.550
ENSG00000055732 E023 1.3385568 0.013696863 9.150087e-01   1 85047310 85047574 265 - 0.360 0.345 -0.090
ENSG00000055732 E024 1.2782695 0.013361896 9.283649e-01   1 85047575 85047609 35 - 0.360 0.344 -0.091
ENSG00000055732 E025 3.2993818 0.007996429 7.120014e-01 8.430896e-01 1 85048396 85048500 105 - 0.639 0.583 -0.246