ENSG00000050426

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262055 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding protein_coding 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 9.134526 9.448659 10.1591025 0.01412415 0.5904421 0.10448473 0.23154167 0.25363333 0.28160000 0.0279666667 0.935955252 0.003639468 FALSE  
ENST00000547318 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding nonsense_mediated_decay 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 2.410931 2.144109 2.6457982 0.38059792 0.4268813 0.30205425 0.06115417 0.05580000 0.07053333 0.0147333333 0.740463603 0.003639468 FALSE  
ENST00000547877 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding retained_intron 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 2.600564 2.449387 2.6691779 0.62200521 0.7078712 0.12349156 0.06445833 0.06303333 0.06950000 0.0064666667 0.971821312 0.003639468    
ENST00000549340 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding protein_coding 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 1.381553 0.000000 2.3315848 0.00000000 1.2064296 7.87134148 0.03509583 0.00000000 0.05786667 0.0578666667 0.253794755 0.003639468 FALSE  
ENST00000550100 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding nonsense_mediated_decay 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 3.236872 4.986811 2.3114849 0.87732139 1.2093891 -1.10595971 0.08100417 0.13740000 0.06470000 -0.0727000000 0.788249108 0.003639468 TRUE  
ENST00000553043 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding protein_coding 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 1.832824 1.988070 0.0651615 0.52179022 0.0651615 -4.73246921 0.04472083 0.05273333 0.00160000 -0.0511333333 0.003639468 0.003639468    
ENST00000623495 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding TEC 40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 2.330968 2.013862 1.9827439 0.83763392 0.5926333 -0.02235469 0.05632917 0.05066667 0.05130000 0.0006333333 1.000000000 0.003639468    
MSTRG.7300.10 ENSG00000050426 HEK293_OSMI2_6hA HEK293_TMG_6hB LETMD1 protein_coding   40.11231 37.99931 37.08682 3.679812 3.218198 -0.03505731 2.462201 4.660168 1.2439929 1.23067361 1.2439929 -1.89694520 0.06199167 0.12033333 0.04060000 -0.0797333333 0.257571411 0.003639468 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000050426 E001 0.4812263 4.1250846147 1.000000e+00   12 51047962 51048298 337 + 0.165 0.165 -0.004
ENSG00000050426 E002 0.6300180 2.4354176681 3.937536e-01   12 51048301 51048312 12 + 0.255 0.178 -0.657
ENSG00000050426 E003 0.6300180 2.4354176681 3.937536e-01   12 51048313 51048316 4 + 0.255 0.178 -0.657
ENSG00000050426 E004 0.8327523 1.7200263399 5.084595e-01   12 51048317 51048317 1 + 0.257 0.253 -0.034
ENSG00000050426 E005 0.8327523 1.7200263399 5.084595e-01   12 51048318 51048318 1 + 0.257 0.253 -0.034
ENSG00000050426 E006 0.8327523 1.7200263399 5.084595e-01   12 51048319 51048319 1 + 0.257 0.253 -0.034
ENSG00000050426 E007 0.9753869 1.6643945079 4.281443e-01   12 51048320 51048320 1 + 0.342 0.252 -0.606
ENSG00000050426 E008 1.3091769 1.0313983534 3.183530e-01   12 51048321 51048326 6 + 0.421 0.309 -0.657
ENSG00000050426 E009 1.6842855 1.0811429336 4.660882e-01 0.6773183139 12 51048327 51048328 2 + 0.421 0.413 -0.044
ENSG00000050426 E010 3.5770347 0.0109497047 1.000000e+00 1.0000000000 12 51048329 51048337 9 + 0.601 0.668 0.293
ENSG00000050426 E011 8.0193419 0.0039529087 9.114791e-01 0.9579129715 12 51048338 51048340 3 + 0.917 0.956 0.147
ENSG00000050426 E012 8.4957666 0.0038519006 1.000000e+00 1.0000000000 12 51048341 51048342 2 + 0.960 0.968 0.030
ENSG00000050426 E013 10.0856485 0.0038963653 7.978653e-01 0.8951245483 12 51048343 51048343 1 + 1.051 1.021 -0.109
ENSG00000050426 E014 22.2404597 0.0022315188 7.910498e-02 0.2430417396 12 51048344 51048345 2 + 1.432 1.301 -0.453
ENSG00000050426 E015 39.5513802 0.0069367489 2.983391e-01 0.5387710833 12 51048346 51048357 12 + 1.638 1.572 -0.225
ENSG00000050426 E016 53.5882337 0.0009616689 6.744731e-01 0.8191867521 12 51048358 51048371 14 + 1.739 1.717 -0.072
ENSG00000050426 E017 109.1116080 0.0050236547 8.250548e-01 0.9109741453 12 51048372 51048478 107 + 2.018 2.033 0.050
ENSG00000050426 E018 13.3448590 0.0505231473 9.814598e-01 0.9925198188 12 51048479 51048518 40 + 1.147 1.148 0.005
ENSG00000050426 E019 46.1647480 0.0496792326 2.656294e-01 0.5050347071 12 51048519 51048873 355 + 1.718 1.622 -0.327
ENSG00000050426 E020 25.1859644 0.0263046496 7.228155e-02 0.2293667708 12 51048874 51049012 139 + 1.500 1.338 -0.560
ENSG00000050426 E021 11.3118672 0.0101343878 1.824921e-01 0.4072399425 12 51049013 51049033 21 + 1.153 1.014 -0.504
ENSG00000050426 E022 96.6509951 0.0039660911 5.832504e-01 0.7591541381 12 51049034 51049077 44 + 1.989 1.964 -0.083
ENSG00000050426 E023 143.3383473 0.0018010780 1.382118e-01 0.3448096364 12 51049078 51049185 108 + 2.176 2.123 -0.176
ENSG00000050426 E024 16.4489704 0.1795503508 9.184214e-02 0.2670967264 12 51049186 51049430 245 + 1.377 1.112 -0.936
ENSG00000050426 E025 10.1629151 0.3676792652 1.444041e-01 0.3539968656 12 51052088 51052091 4 + 1.176 0.927 -0.912
ENSG00000050426 E026 85.5630025 0.0006488321 1.706014e-01 0.3912655034 12 51052092 51052207 116 + 1.959 1.904 -0.185
ENSG00000050426 E027 8.3548332 0.0199080211 5.600411e-02 0.1936550385 12 51052208 51052246 39 + 1.096 0.866 -0.856
ENSG00000050426 E028 9.4241872 0.0663508211 1.194067e-02 0.0662030666 12 51052247 51052361 115 + 1.225 0.830 -1.456
ENSG00000050426 E029 54.5362549 0.0008083652 6.415795e-01 0.7978716331 12 51053778 51053811 34 + 1.745 1.721 -0.081
ENSG00000050426 E030 69.0319784 0.0006473467 2.154745e-01 0.4480096107 12 51053812 51053860 49 + 1.793 1.848 0.186
ENSG00000050426 E031 10.4468137 0.0716082655 2.046658e-01 0.4354908557 12 51055699 51055834 136 + 1.145 0.982 -0.591
ENSG00000050426 E032 70.7263787 0.0005627643 2.206007e-01 0.4542092642 12 51055835 51055879 45 + 1.805 1.858 0.181
ENSG00000050426 E033 61.5916805 0.0006373596 8.209656e-01 0.9085975787 12 51055880 51055908 29 + 1.771 1.782 0.035
ENSG00000050426 E034 91.7936560 0.0004784196 6.585286e-01 0.8090378172 12 51055909 51055986 78 + 1.961 1.944 -0.060
ENSG00000050426 E035 82.7487452 0.0008713716 1.392643e-02 0.0740552647 12 51055987 51056021 35 + 1.967 1.865 -0.343
ENSG00000050426 E036 8.0646424 0.0944398339 5.913451e-02 0.2008399576 12 51056022 51056056 35 + 1.105 0.813 -1.095
ENSG00000050426 E037 12.5896316 0.0928398880 3.248158e-01 0.5640390376 12 51056057 51056143 87 + 1.184 1.072 -0.403
ENSG00000050426 E038 70.0204374 0.0007151337 1.614703e-01 0.3789575441 12 51056144 51056149 6 + 1.872 1.809 -0.211
ENSG00000050426 E039 85.9366956 0.0005427930 2.036486e-01 0.4341152632 12 51056150 51056178 29 + 1.953 1.902 -0.173
ENSG00000050426 E040 127.0292947 0.0006767276 5.729823e-01 0.7523096320 12 51056179 51056245 67 + 2.103 2.083 -0.067
ENSG00000050426 E041 13.7690127 0.0683186179 6.763112e-02 0.2197390436 12 51056246 51056349 104 + 1.301 1.040 -0.929
ENSG00000050426 E042 125.0996414 0.0009833225 1.430979e-01 0.3521393513 12 51056350 51056370 21 + 2.052 2.104 0.176
ENSG00000050426 E043 220.7279294 0.0019029576 2.578235e-01 0.4966037282 12 51056371 51056502 132 + 2.304 2.344 0.135
ENSG00000050426 E044 16.8649845 0.0129950338 7.054791e-01 0.8390150342 12 51056503 51056679 177 + 1.255 1.228 -0.092
ENSG00000050426 E045 17.1226339 0.0229605801 1.000000e+00 1.0000000000 12 51056680 51056952 273 + 1.222 1.254 0.112
ENSG00000050426 E046 33.3634170 0.0992910352 2.096234e-01 0.4414379161 12 51057471 51058005 535 + 1.600 1.469 -0.447
ENSG00000050426 E047 9.2078966 0.0037491897 5.479004e-02 0.1908637902 12 51058006 51058031 26 + 1.112 0.907 -0.757
ENSG00000050426 E048 121.9616547 0.0020634056 3.921773e-01 0.6221440750 12 51058032 51058074 43 + 2.050 2.086 0.120
ENSG00000050426 E049 128.6818184 0.0012140585 1.215348e-01 0.3182794096 12 51058075 51058128 54 + 2.054 2.113 0.196
ENSG00000050426 E050 21.8628116 0.1393550185 2.841790e-01 0.5246859098 12 51058129 51058603 475 + 1.402 1.306 -0.333
ENSG00000050426 E051 10.4162254 0.0681939128 1.000000e+00 1.0000000000 12 51058604 51058738 135 + 1.028 1.049 0.079
ENSG00000050426 E052 51.8381212 0.0573452859 3.371912e-01 0.5755819784 12 51058739 51059360 622 + 1.742 1.685 -0.194
ENSG00000050426 E053 735.4780890 0.0021324243 2.751988e-05 0.0005003221 12 51059361 51060424 1064 + 2.776 2.886 0.365