ENSG00000035403

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000372755 ENSG00000035403 HEK293_OSMI2_6hA HEK293_TMG_6hB VCL protein_coding protein_coding 42.59755 30.99357 49.83356 5.313943 1.818548 0.6849728 31.949882 26.881450 32.84265 5.8647676 0.303186 0.2888621 0.7790083 0.8516000 0.6610333 -0.1905667 0.19706791 0.02056301 FALSE TRUE
MSTRG.4209.2 ENSG00000035403 HEK293_OSMI2_6hA HEK293_TMG_6hB VCL protein_coding   42.59755 30.99357 49.83356 5.313943 1.818548 0.6849728 9.783737 3.128575 15.83848 0.6710057 1.722093 2.3361634 0.1975750 0.1083333 0.3162000 0.2078667 0.02056301 0.02056301 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000035403 E001 0.1614157 0.0343835297 5.864068e-01   10 73995193 73995231 39 + 0.108 0.000 -9.061
ENSG00000035403 E002 0.1614157 0.0343835297 5.864068e-01   10 73997182 73997221 40 + 0.108 0.000 -11.457
ENSG00000035403 E003 9.1734461 0.0574150342 8.929048e-01 9.480791e-01 10 73998104 73998113 10 + 0.979 1.054 0.276
ENSG00000035403 E004 9.1734461 0.0574150342 8.929048e-01 9.480791e-01 10 73998114 73998115 2 + 0.979 1.054 0.276
ENSG00000035403 E005 221.7997051 0.0002947792 2.215030e-01 4.552764e-01 10 73998116 73998375 260 + 2.344 2.344 0.001
ENSG00000035403 E006 0.0000000       10 74016832 74016878 47 +      
ENSG00000035403 E007 72.4285560 0.0066866207 7.806918e-01 8.851551e-01 10 74043083 74043090 8 + 1.850 1.870 0.065
ENSG00000035403 E008 139.3396422 0.0004414032 7.984008e-01 8.953821e-01 10 74043091 74043153 63 + 2.132 2.154 0.073
ENSG00000035403 E009 0.1308682 0.0308807501 4.054369e-01   10 74063686 74063890 205 + 0.000 0.136 11.238
ENSG00000035403 E010 257.9330155 0.0001905912 3.236164e-04 3.969484e-03 10 74070670 74070820 151 + 2.432 2.380 -0.172
ENSG00000035403 E011 244.8714991 0.0002434008 1.538044e-08 6.917249e-07 10 74070975 74071083 109 + 2.430 2.326 -0.347
ENSG00000035403 E012 254.9803627 0.0002606884 8.231445e-08 3.120984e-06 10 74072730 74072852 123 + 2.443 2.347 -0.317
ENSG00000035403 E013 332.3223098 0.0002058774 2.361850e-10 1.547440e-08 10 74074743 74074903 161 + 2.559 2.460 -0.330
ENSG00000035403 E014 1.8371591 0.2679365516 3.637916e-01 5.988830e-01 10 74074904 74077748 2845 + 0.518 0.327 -1.037
ENSG00000035403 E015 291.3261651 0.0003567560 1.693222e-04 2.327947e-03 10 74082454 74082544 91 + 2.486 2.432 -0.178
ENSG00000035403 E016 362.4566827 0.0001634068 2.094441e-04 2.777699e-03 10 74083366 74083513 148 + 2.575 2.533 -0.138
ENSG00000035403 E017 351.6924076 0.0017385906 1.097517e-01 2.986299e-01 10 74089196 74089349 154 + 2.551 2.538 -0.044
ENSG00000035403 E018 388.6556832 0.0004425586 4.627286e-03 3.262016e-02 10 74090023 74090198 176 + 2.601 2.573 -0.090
ENSG00000035403 E019 414.6319413 0.0001410233 3.902132e-01 6.206155e-01 10 74094271 74094461 191 + 2.608 2.623 0.048
ENSG00000035403 E020 392.7849242 0.0002069087 2.132966e-01 4.456090e-01 10 74095656 74095855 200 + 2.589 2.595 0.021
ENSG00000035403 E021 304.3187908 0.0015152830 6.389326e-02 2.114663e-01 10 74097204 74097332 129 + 2.490 2.469 -0.071
ENSG00000035403 E022 318.4053722 0.0014678256 2.623465e-02 1.160372e-01 10 74100948 74101053 106 + 2.514 2.483 -0.103
ENSG00000035403 E023 215.3789218 0.0002266891 9.383301e-01 9.714689e-01 10 74101054 74101097 44 + 2.316 2.347 0.105
ENSG00000035403 E024 300.9848028 0.0001680074 1.511180e-02 7.852670e-02 10 74103820 74103928 109 + 2.485 2.464 -0.070
ENSG00000035403 E025 2.8148787 0.1128471801 7.672847e-01 8.769747e-01 10 74104928 74105050 123 + 0.550 0.630 0.358
ENSG00000035403 E026 549.6354406 0.0001389357 1.051684e-03 1.030762e-02 10 74105051 74105353 303 + 2.745 2.723 -0.072
ENSG00000035403 E027 323.2464400 0.0001980966 5.547407e-01 7.395851e-01 10 74107230 74107354 125 + 2.500 2.517 0.059
ENSG00000035403 E028 397.9672067 0.0002593044 8.877850e-01 9.454541e-01 10 74108971 74109156 186 + 2.584 2.616 0.108
ENSG00000035403 E029 12.5039042 0.1556120888 2.713707e-01 5.113168e-01 10 74111362 74111523 162 + 0.975 1.264 1.044
ENSG00000035403 E030 15.1390275 0.2495670964 5.648000e-01 7.466375e-01 10 74111909 74112112 204 + 1.072 1.331 0.919
ENSG00000035403 E031 434.1306331 0.0007538926 4.973515e-03 3.448825e-02 10 74114184 74114387 204 + 2.595 2.683 0.293
ENSG00000035403 E032 325.1416594 0.0019895346 1.317867e-02 7.116186e-02 10 74114795 74114899 105 + 2.464 2.565 0.336
ENSG00000035403 E033 2406.0661167 0.0016600707 4.162307e-11 3.191221e-09 10 74118023 74121363 3341 + 3.309 3.452 0.475