ENSG00000034053

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000411764 ENSG00000034053 HEK293_OSMI2_6hA HEK293_TMG_6hB APBA2 protein_coding protein_coding 13.01861 18.07079 8.034982 2.21208 0.1212243 -1.168296 4.163813 7.654129 1.478972 0.9752415 0.75740829 -2.3638056 0.2780083 0.4236333 0.1826333 -0.24100000 0.67224901 0.02435785 FALSE TRUE
ENST00000559814 ENSG00000034053 HEK293_OSMI2_6hA HEK293_TMG_6hB APBA2 protein_coding retained_intron 13.01861 18.07079 8.034982 2.21208 0.1212243 -1.168296 4.040289 4.894804 3.694526 0.8029226 0.23916257 -0.4049065 0.3382458 0.2706000 0.4596333 0.18903333 0.02435785 0.02435785 FALSE TRUE
MSTRG.10344.5 ENSG00000034053 HEK293_OSMI2_6hA HEK293_TMG_6hB APBA2 protein_coding   13.01861 18.07079 8.034982 2.21208 0.1212243 -1.168296 1.679549 1.676945 1.541956 0.2951045 0.32048552 -0.1203252 0.1407667 0.0956000 0.1924667 0.09686667 0.38500494 0.02435785 FALSE TRUE
MSTRG.10344.7 ENSG00000034053 HEK293_OSMI2_6hA HEK293_TMG_6hB APBA2 protein_coding   13.01861 18.07079 8.034982 2.21208 0.1212243 -1.168296 1.714102 2.712927 0.497626 0.7074402 0.07886873 -2.4233202 0.1253167 0.1445000 0.0621000 -0.08240000 0.18622562 0.02435785 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000034053 E001 0.0000000       15 28884483 28884613 131 +      
ENSG00000034053 E002 0.8148287 0.0169687838 8.013149e-01   15 28885974 28886021 48 + 0.193 0.242 0.418
ENSG00000034053 E003 2.0433082 0.0121711133 1.408307e-01 3.487917e-01 15 28886022 28886087 66 + 0.193 0.485 1.882
ENSG00000034053 E004 2.0848144 0.0175832727 1.411407e-01 3.492386e-01 15 28886088 28886089 2 + 0.193 0.486 1.885
ENSG00000034053 E005 2.6801531 0.0091320969 5.023296e-02 1.802428e-01 15 28886090 28886118 29 + 0.193 0.580 2.328
ENSG00000034053 E006 5.6433102 0.0050834516 1.275392e-01 3.279543e-01 15 28886119 28886159 41 + 0.579 0.819 1.003
ENSG00000034053 E007 5.5466937 0.0056992252 2.780262e-01 5.181201e-01 15 28886160 28886162 3 + 0.638 0.806 0.689
ENSG00000034053 E008 33.5642069 0.0063597982 4.053720e-01 6.326085e-01 15 28886163 28886278 116 + 1.524 1.460 -0.220
ENSG00000034053 E009 0.0000000       15 28893874 28894044 171 +      
ENSG00000034053 E010 0.6082027 0.8831409395 7.964619e-01   15 28895520 28895609 90 + 0.000 0.211 9.537
ENSG00000034053 E011 0.0000000       15 28919367 28919487 121 +      
ENSG00000034053 E012 0.0000000       15 28921637 28921639 3 +      
ENSG00000034053 E013 51.2572202 0.0013224586 5.851933e-03 3.901246e-02 15 28921640 28921718 79 + 1.763 1.612 -0.512
ENSG00000034053 E014 41.3390232 0.0010160241 6.686166e-03 4.312765e-02 15 28921719 28921749 31 + 1.680 1.520 -0.547
ENSG00000034053 E015 0.0000000       15 28961304 28961584 281 +      
ENSG00000034053 E016 0.0000000       15 28990419 28990796 378 +      
ENSG00000034053 E017 57.6027731 0.0035231675 1.323888e-02 7.140528e-02 15 28995753 28995806 54 + 1.807 1.664 -0.482
ENSG00000034053 E018 15.1282027 0.0130627553 7.376955e-04 7.763861e-03 15 29014091 29014280 190 + 1.379 1.036 -1.218
ENSG00000034053 E019 0.0000000       15 29044324 29044428 105 +      
ENSG00000034053 E020 355.7031341 0.0003082485 1.030868e-01 2.873404e-01 15 29053845 29054835 991 + 2.516 2.482 -0.112
ENSG00000034053 E021 0.0000000       15 29070658 29071099 442 +      
ENSG00000034053 E022 74.1472503 0.0030553676 1.344189e-01 3.387562e-01 15 29074921 29075001 81 + 1.869 1.789 -0.270
ENSG00000034053 E023 55.7698010 0.0006809541 1.298136e-01 3.315487e-01 15 29076055 29076091 37 + 1.750 1.671 -0.268
ENSG00000034053 E024 98.5749477 0.0004857650 6.414246e-02 2.119936e-01 15 29093075 29093220 146 + 1.991 1.919 -0.243
ENSG00000034053 E025 0.2735028 0.0257628324 4.241576e-01   15 29094278 29094313 36 + 0.192 0.074 -1.581
ENSG00000034053 E026 86.7016143 0.0005158668 5.085570e-03 3.508050e-02 15 29098490 29098576 87 + 1.966 1.851 -0.389
ENSG00000034053 E027 145.3529001 0.0004029557 1.279114e-01 3.285134e-01 15 29101599 29101784 186 + 2.142 2.093 -0.164
ENSG00000034053 E028 21.0218964 0.0696891478 1.328807e-01 3.363206e-01 15 29101785 29102759 975 + 1.427 1.217 -0.732
ENSG00000034053 E029 187.2480895 0.0002540962 9.954841e-01 9.993149e-01 15 29105379 29105558 180 + 2.213 2.214 0.002
ENSG00000034053 E030 196.1199752 0.0003702021 6.435050e-01 7.992127e-01 15 29106607 29106819 213 + 2.222 2.235 0.045
ENSG00000034053 E031 155.9206746 0.0019260429 1.101422e-01 2.992318e-01 15 29108270 29108389 120 + 2.086 2.147 0.204
ENSG00000034053 E032 17.3328005 0.0095791043 2.063336e-01 4.373681e-01 15 29108390 29108620 231 + 1.105 1.242 0.487
ENSG00000034053 E033 171.0972047 0.0018259409 1.396742e-04 1.976822e-03 15 29113876 29114016 141 + 2.062 2.201 0.466
ENSG00000034053 E034 326.2771972 0.0002631490 1.460781e-11 1.219638e-09 15 29117062 29118806 1745 + 2.332 2.488 0.520