ENSG00000033178

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000322244 ENSG00000033178 HEK293_OSMI2_6hA HEK293_TMG_6hB UBA6 protein_coding protein_coding 19.34573 2.38278 37.68179 0.172194 2.084873 3.977491 1.71487465 0.6531774 2.177809 0.1147842 0.6976401 1.722020 0.21513750 0.2696667 0.05706667 -0.2126 1.026917e-02 8.794771e-18 FALSE TRUE
ENST00000506571 ENSG00000033178 HEK293_OSMI2_6hA HEK293_TMG_6hB UBA6 protein_coding retained_intron 19.34573 2.38278 37.68179 0.172194 2.084873 3.977491 7.74755950 0.0000000 15.195105 0.0000000 1.1906885 10.570340 0.22307083 0.0000000 0.40290000 0.4029 8.794771e-18 8.794771e-18 FALSE TRUE
ENST00000514261 ENSG00000033178 HEK293_OSMI2_6hA HEK293_TMG_6hB UBA6 protein_coding nonsense_mediated_decay 19.34573 2.38278 37.68179 0.172194 2.084873 3.977491 0.05447338 0.1497220 0.000000 0.1497220 0.0000000 -3.997491 0.01465417 0.0734000 0.00000000 -0.0734 5.061739e-01 8.794771e-18 TRUE FALSE
MSTRG.24971.2 ENSG00000033178 HEK293_OSMI2_6hA HEK293_TMG_6hB UBA6 protein_coding   19.34573 2.38278 37.68179 0.172194 2.084873 3.977491 5.96585069 1.4363706 11.312057 0.2388292 1.6459368 2.968627 0.42215000 0.5942667 0.29966667 -0.2946 4.433337e-02 8.794771e-18 FALSE TRUE
MSTRG.24971.3 ENSG00000033178 HEK293_OSMI2_6hA HEK293_TMG_6hB UBA6 protein_coding   19.34573 2.38278 37.68179 0.172194 2.084873 3.977491 3.58926412 0.0000000 8.326889 0.0000000 1.1786370 9.703365 0.09847500 0.0000000 0.22280000 0.2228 4.942224e-08 8.794771e-18 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000033178 E001 141.6759322 0.1004340272 1.128153e-03 0.010876939 4 67612652 67617446 4795 - 1.838 2.382 1.824
ENSG00000033178 E002 266.9773940 1.7083493724 3.591923e-01 0.595200396 4 67617447 67618668 1222 - 2.134 2.586 1.508
ENSG00000033178 E003 73.7219776 1.0237605930 3.683452e-01 0.602669607 4 67618669 67618854 186 - 1.611 1.930 1.078
ENSG00000033178 E004 106.4424022 1.4251605265 4.861813e-01 0.691375674 4 67618855 67619132 278 - 1.775 2.049 0.920
ENSG00000033178 E005 79.1805583 0.0376243103 1.517661e-01 0.364877376 4 67622831 67622925 95 - 1.657 1.891 0.793
ENSG00000033178 E006 84.7045738 0.0034191834 9.253269e-01 0.964870527 4 67623135 67623222 88 - 1.704 1.818 0.386
ENSG00000033178 E007 99.6533979 0.0020663208 8.449995e-01 0.922149816 4 67624126 67624253 128 - 1.770 1.894 0.420
ENSG00000033178 E008 109.2371844 0.0057456385 9.577212e-01 0.980699250 4 67624994 67625187 194 - 1.811 1.925 0.384
ENSG00000033178 E009 91.6884478 0.0124176235 4.320708e-02 0.163100285 4 67626360 67626477 118 - 1.752 1.707 -0.152
ENSG00000033178 E010 66.2692108 0.0186758605 4.671036e-02 0.171851501 4 67629071 67629142 72 - 1.618 1.545 -0.249
ENSG00000033178 E011 0.1614157 0.0322560722 7.807865e-01   4 67630454 67630465 12 - 0.044 0.001 -6.491
ENSG00000033178 E012 59.7131087 0.0125630590 1.016009e-01 0.284661121 4 67630466 67630535 70 - 1.570 1.544 -0.089
ENSG00000033178 E013 52.7568301 0.0118352471 5.605687e-01 0.743690647 4 67631708 67631771 64 - 1.493 1.662 0.576
ENSG00000033178 E014 51.3791117 0.0228563576 6.095584e-01 0.776612754 4 67631857 67631908 52 - 1.482 1.659 0.603
ENSG00000033178 E015 79.6999861 0.0074236727 7.569514e-01 0.870825356 4 67633345 67633473 129 - 1.680 1.781 0.341
ENSG00000033178 E016 64.3458984 0.0051886734 6.898454e-01 0.828934217 4 67634242 67634322 81 - 1.592 1.680 0.300
ENSG00000033178 E017 78.7933196 0.0105954520 4.542667e-01 0.668939754 4 67634429 67634518 90 - 1.678 1.744 0.223
ENSG00000033178 E018 78.7499266 0.0063465874 7.503892e-01 0.866958335 4 67635453 67635558 106 - 1.673 1.769 0.327
ENSG00000033178 E019 102.3416651 0.0041350956 1.513132e-01 0.364251572 4 67638943 67639124 182 - 1.792 1.818 0.089
ENSG00000033178 E020 59.0687657 0.0056134349 3.878809e-01 0.618834453 4 67641151 67641228 78 - 1.557 1.604 0.162
ENSG00000033178 E021 56.4971244 0.0096052468 2.754193e-01 0.515476507 4 67644698 67644778 81 - 1.540 1.560 0.068
ENSG00000033178 E022 45.0073213 0.0315771710 3.200581e-01 0.559809128 4 67645938 67646016 79 - 1.447 1.439 -0.028
ENSG00000033178 E023 41.6573570 0.0181183781 1.002638e-01 0.282270199 4 67646724 67646791 68 - 1.420 1.354 -0.231
ENSG00000033178 E024 59.7293899 0.0071783635 1.372898e-02 0.073276015 4 67649068 67649211 144 - 1.576 1.482 -0.321
ENSG00000033178 E025 1.7274571 0.1240364745 9.716360e-03 0.056884940 4 67661663 67662188 526 - 0.185 0.780 3.238
ENSG00000033178 E026 52.7407202 0.0009408716 4.547696e-03 0.032190036 4 67662189 67662255 67 - 1.526 1.406 -0.412
ENSG00000033178 E027 2.5575911 0.0492905362 9.405670e-01 0.972542101 4 67662256 67662261 6 - 0.395 0.473 0.412
ENSG00000033178 E028 3.5867483 0.0221497001 8.443107e-01 0.921741686 4 67662487 67663138 652 - 0.480 0.596 0.547
ENSG00000033178 E029 54.2894246 0.0011289883 4.956802e-03 0.034403465 4 67663139 67663215 77 - 1.537 1.423 -0.393
ENSG00000033178 E030 3.4085929 0.0399420607 8.399596e-01 0.919355593 4 67663216 67663884 669 - 0.466 0.595 0.611
ENSG00000033178 E031 54.4197571 0.0085860638 1.145265e-02 0.064217056 4 67663885 67663947 63 - 1.538 1.422 -0.397
ENSG00000033178 E032 68.2415799 0.0170504864 8.175181e-02 0.248311371 4 67665189 67665292 104 - 1.626 1.582 -0.151
ENSG00000033178 E033 95.6151876 0.0241829324 2.047318e-03 0.017378648 4 67668551 67668674 124 - 1.781 1.594 -0.634
ENSG00000033178 E034 99.4906887 0.0154165696 1.472391e-03 0.013420236 4 67670470 67670592 123 - 1.796 1.643 -0.518
ENSG00000033178 E035 73.6747181 0.0043440380 1.065270e-04 0.001577359 4 67673697 67673777 81 - 1.672 1.482 -0.649
ENSG00000033178 E036 83.0151710 0.0107901990 2.045025e-05 0.000389345 4 67677611 67677722 112 - 1.726 1.467 -0.883
ENSG00000033178 E037 0.1426347 0.0320455500 7.802738e-01   4 67678430 67678438 9 - 0.044 0.001 -6.489
ENSG00000033178 E038 74.5831605 0.0088902155 4.624532e-04 0.005331865 4 67678439 67678533 95 - 1.676 1.495 -0.617
ENSG00000033178 E039 49.4259087 0.0085930647 1.801090e-02 0.089067446 4 67681563 67681591 29 - 1.496 1.389 -0.370
ENSG00000033178 E040 61.1095340 0.0222448467 5.946079e-03 0.039477409 4 67682119 67682213 95 - 1.590 1.406 -0.633
ENSG00000033178 E041 44.6826945 0.0174075469 3.072371e-02 0.129516674 4 67696645 67696707 63 - 1.454 1.333 -0.416
ENSG00000033178 E042 37.8856406 0.0235520186 2.449131e-02 0.110640234 4 67701049 67701155 107 - 1.388 1.222 -0.580