ENSG00000028116

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000340157 ENSG00000028116 HEK293_OSMI2_6hA HEK293_TMG_6hB VRK2 protein_coding protein_coding 11.03366 4.128719 18.72338 0.7867627 0.604874 2.178355 8.0195513 3.477963 14.646032 0.6933618 0.2807008 2.071038 0.73995417 0.8418 0.78293333 -0.05886667 0.57161438 0.01602439 FALSE  
MSTRG.18464.10 ENSG00000028116 HEK293_OSMI2_6hA HEK293_TMG_6hB VRK2 protein_coding   11.03366 4.128719 18.72338 0.7867627 0.604874 2.178355 0.7685673 0.000000 1.112585 0.0000000 0.5406691 6.810680 0.06710417 0.0000 0.05943333 0.05943333 0.01602439 0.01602439 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000028116 E001 0.0000000       2 57907629 57907650 22 +      
ENSG00000028116 E002 0.0000000       2 57907651 57907651 1 +      
ENSG00000028116 E003 0.0000000       2 57907652 57907655 4 +      
ENSG00000028116 E004 0.0000000       2 57907656 57907839 184 +      
ENSG00000028116 E005 0.0000000       2 58018016 58018086 71 +      
ENSG00000028116 E006 0.0000000       2 58025665 58025770 106 +      
ENSG00000028116 E007 0.0000000       2 58026744 58026861 118 +      
ENSG00000028116 E008 0.0000000       2 58028298 58028398 101 +      
ENSG00000028116 E009 0.0000000       2 58033227 58033553 327 +      
ENSG00000028116 E010 0.0000000       2 58033554 58033663 110 +      
ENSG00000028116 E011 0.3559677 0.6748618361 1.000000000   2 58046292 58046532 241 + 0.135 0.000 -9.660
ENSG00000028116 E012 0.0000000       2 58046533 58046627 95 +      
ENSG00000028116 E013 0.0000000       2 58046628 58046633 6 +      
ENSG00000028116 E014 0.0000000       2 58046634 58046638 5 +      
ENSG00000028116 E015 0.1426347 0.0324189773 1.000000000   2 58046639 58046641 3 + 0.070 0.000 -10.334
ENSG00000028116 E016 0.7319543 0.1847186618 0.325003849   2 58046642 58046691 50 + 0.270 0.000 -12.667
ENSG00000028116 E017 1.9271617 0.2354921202 0.081952439 0.24865665 2 58046692 58046711 20 + 0.506 0.000 -13.957
ENSG00000028116 E018 2.5518305 0.1502155484 0.026603308 0.11707924 2 58046712 58046714 3 + 0.592 0.000 -14.327
ENSG00000028116 E019 5.4592323 0.0056731064 0.005752596 0.03850854 2 58046715 58046805 91 + 0.832 0.340 -2.287
ENSG00000028116 E020 6.6410594 0.0046793991 0.001181186 0.01129017 2 58046806 58046821 16 + 0.904 0.340 -2.566
ENSG00000028116 E021 18.1111799 0.0019879907 0.591376687 0.76465759 2 58046822 58046868 47 + 1.202 1.259 0.202
ENSG00000028116 E022 0.1779838 0.0494342291 1.000000000   2 58046869 58046874 6 + 0.070 0.000 -10.354
ENSG00000028116 E023 3.2290636 0.0104194921 0.314349312 0.55455397 2 58046875 58046941 67 + 0.539 0.711 0.751
ENSG00000028116 E024 4.9015532 0.0080575306 0.549244815 0.73580551 2 58046942 58047026 85 + 0.702 0.800 0.397
ENSG00000028116 E025 6.0130579 0.0312246655 0.847669009 0.92366823 2 58047027 58047128 102 + 0.785 0.835 0.198
ENSG00000028116 E026 0.6731895 0.0512308824 0.956949893   2 58048527 58048587 61 + 0.184 0.202 0.168
ENSG00000028116 E027 35.6547375 0.0023330602 0.660292016 0.81014349 2 58048827 58048880 54 + 1.499 1.477 -0.075
ENSG00000028116 E028 60.1373478 0.0129718456 0.421495149 0.64475063 2 58048881 58048967 87 + 1.726 1.681 -0.153
ENSG00000028116 E029 4.7093839 0.0061154571 0.579163540 0.75660130 2 58058352 58058488 137 + 0.671 0.759 0.364
ENSG00000028116 E030 63.2119463 0.0111145128 0.292764823 0.53347242 2 58084089 58084138 50 + 1.751 1.687 -0.216
ENSG00000028116 E031 71.4452507 0.0161296859 0.187903057 0.41428984 2 58084881 58084950 70 + 1.808 1.720 -0.295
ENSG00000028116 E032 2.3735838 0.3053286437 0.060642480 0.20425808 2 58084951 58084999 49 + 0.551 0.000 -14.141
ENSG00000028116 E033 73.6327924 0.0088475176 0.377199427 0.61002603 2 58086339 58086426 88 + 1.813 1.768 -0.150
ENSG00000028116 E034 73.0685888 0.0014629542 0.442292787 0.66027888 2 58088341 58088446 106 + 1.791 1.835 0.151
ENSG00000028116 E035 69.6105548 0.0014732302 0.444773534 0.66197011 2 58089631 58089723 93 + 1.769 1.816 0.159
ENSG00000028116 E036 87.1362844 0.0017791951 0.965276342 0.98453537 2 58123101 58123233 133 + 1.874 1.888 0.044
ENSG00000028116 E037 74.8457914 0.0009241468 0.668429163 0.81516255 2 58131808 58131928 121 + 1.818 1.808 -0.034
ENSG00000028116 E038 49.8557268 0.0008594454 0.295048628 0.53556763 2 58135141 58135199 59 + 1.628 1.695 0.228
ENSG00000028116 E039 98.8164540 0.0004181366 0.772505757 0.88016937 2 58139666 58139832 167 + 1.933 1.930 -0.011
ENSG00000028116 E040 97.1245085 0.0170589615 0.623845185 0.78649588 2 58146316 58146474 159 + 1.918 1.935 0.059
ENSG00000028116 E041 2.6299033 0.0446355045 0.656497788 0.80763412 2 58154754 58154758 5 + 0.536 0.444 -0.453
ENSG00000028116 E042 6.8810782 0.0579340511 0.134483305 0.33884805 2 58154759 58154802 44 + 0.778 0.996 0.834
ENSG00000028116 E043 101.6894045 1.3930685245 0.472423148 0.68184639 2 58159349 58159920 572 + 1.897 2.055 0.528