ENSG00000026652

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000320285 ENSG00000026652 HEK293_OSMI2_6hA HEK293_TMG_6hB AGPAT4 protein_coding protein_coding 14.93221 18.88123 12.17289 0.5260997 0.1796341 -0.6328609 2.0101295 2.1248905 1.8682609 0.3098208 0.18748960 -0.1847646 0.14050000 0.1135667 0.15310000 0.03953333 0.668965227 0.008990875 FALSE TRUE
ENST00000366911 ENSG00000026652 HEK293_OSMI2_6hA HEK293_TMG_6hB AGPAT4 protein_coding protein_coding 14.93221 18.88123 12.17289 0.5260997 0.1796341 -0.6328609 0.7777997 0.5003828 0.9135703 0.2505142 0.26606535 0.8556421 0.05419167 0.0258000 0.07553333 0.04973333 0.595909196 0.008990875 FALSE TRUE
MSTRG.29288.3 ENSG00000026652 HEK293_OSMI2_6hA HEK293_TMG_6hB AGPAT4 protein_coding   14.93221 18.88123 12.17289 0.5260997 0.1796341 -0.6328609 10.9858569 14.4512742 7.8445192 0.4229230 0.03843826 -0.8805998 0.72691250 0.7653000 0.64476667 -0.12053333 0.008990875 0.008990875 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000026652 E001 0.2027342 0.0394455197 7.757031e-01   6 161129967 161129978 12 - 0.000 0.096 8.743
ENSG00000026652 E002 398.7901963 0.0006660446 1.540056e-27 1.017654e-24 6 161129979 161136236 6258 - 2.702 2.499 -0.676
ENSG00000026652 E003 254.6575508 0.0031434689 7.169625e-04 7.587034e-03 6 161136237 161136634 398 - 2.321 2.424 0.341
ENSG00000026652 E004 167.2896846 0.0005145793 2.319536e-05 4.336312e-04 6 161139422 161139620 199 - 2.129 2.247 0.395
ENSG00000026652 E005 2.2311532 0.0097153637 6.234222e-02 2.080261e-01 6 161143956 161144266 311 - 0.266 0.597 1.803
ENSG00000026652 E006 83.6391457 0.0006360686 5.013112e-02 1.799877e-01 6 161146524 161146599 76 - 1.865 1.939 0.249
ENSG00000026652 E007 50.4274443 0.0068404740 6.131737e-01 7.790674e-01 6 161149187 161149204 18 - 1.680 1.706 0.086
ENSG00000026652 E008 65.9434618 0.0040834368 2.351866e-01 4.711150e-01 6 161149205 161149255 51 - 1.774 1.832 0.196
ENSG00000026652 E009 45.7598206 0.0035627731 3.272311e-02 1.352792e-01 6 161149256 161149289 34 - 1.573 1.697 0.423
ENSG00000026652 E010 108.5240000 0.0004138661 1.355526e-02 7.258148e-02 6 161153346 161153499 154 - 1.969 2.051 0.274
ENSG00000026652 E011 117.3629331 0.0004593352 1.286175e-04 1.843749e-03 6 161154149 161154310 162 - 1.973 2.100 0.424
ENSG00000026652 E012 60.4541918 0.0574520388 1.627757e-01 3.808737e-01 6 161160105 161165576 5472 - 1.855 1.718 -0.465
ENSG00000026652 E013 8.8346347 0.0034594866 4.802714e-01 6.872688e-01 6 161165577 161165713 137 - 1.030 0.948 -0.304
ENSG00000026652 E014 6.7244909 0.0047331073 3.517212e-01 5.888302e-01 6 161165838 161166247 410 - 0.956 0.839 -0.444
ENSG00000026652 E015 115.3060414 0.0004826350 3.709743e-03 2.752730e-02 6 161166248 161166417 170 - 1.987 2.082 0.318
ENSG00000026652 E016 139.2573402 0.0003037045 3.680709e-01 6.024595e-01 6 161232036 161232302 267 - 2.150 2.118 -0.108
ENSG00000026652 E017 49.8042060 0.0008580980 5.190508e-02 1.842109e-01 6 161273938 161274069 132 - 1.754 1.651 -0.350