ENSG00000025434

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000395397 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding protein_coding 12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 2.13848957 4.3196807 1.8323888 1.79095712 0.13033077 -1.2326831 0.183387500 0.22490000 0.28003333 0.055133333 8.627533e-01 4.70847e-09 FALSE TRUE
ENST00000405576 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding protein_coding 12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 1.46441881 0.8489719 0.3242685 0.44304528 0.32426853 -1.3616034 0.106979167 0.05946667 0.04716667 -0.012300000 8.135229e-01 4.70847e-09 FALSE TRUE
ENST00000481889 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding protein_coding 12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 1.23050300 1.8885376 0.6992217 0.52647002 0.02001577 -1.4205800 0.099404167 0.10760000 0.10623333 -0.001366667 1.000000e+00 4.70847e-09 FALSE TRUE
ENST00000487913 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding retained_intron 12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 1.97733070 2.3948274 1.3034813 0.06574972 0.12996009 -0.8725377 0.174295833 0.15010000 0.19780000 0.047700000 7.529624e-01 4.70847e-09 FALSE FALSE
ENST00000527949 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding protein_coding 12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 0.06891883 0.0000000 0.3169244 0.00000000 0.31692438 5.0308851 0.009658333 0.00000000 0.05106667 0.051066667 7.814535e-01 4.70847e-09 FALSE TRUE
MSTRG.5476.16 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding   12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 0.64382711 0.5845944 0.3017316 0.24638365 0.11614396 -0.9316016 0.057416667 0.04103333 0.04640000 0.005366667 9.361750e-01 4.70847e-09 TRUE TRUE
MSTRG.5476.7 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding   12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 1.23951024 2.8594509 0.0000000 0.97907189 0.00000000 -8.1646309 0.089566667 0.15593333 0.00000000 -0.155933333 4.708470e-09 4.70847e-09 FALSE TRUE
MSTRG.5476.8 ENSG00000025434 HEK293_OSMI2_6hA HEK293_TMG_6hB NR1H3 protein_coding   12.31736 17.2427 6.582825 2.923525 0.1974878 -1.387853 1.21477532 1.1184955 0.3738052 0.99179590 0.07995181 -1.5559547 0.091775000 0.05883333 0.05660000 -0.002233333 7.551359e-01 4.70847e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000025434 E001 0.1426347 0.0320986098 0.15303419   11 47248300 47248310 11 + 0.195 0.000 -11.855
ENSG00000025434 E002 0.1426347 0.0320986098 0.15303419   11 47248311 47248355 45 + 0.195 0.000 -14.094
ENSG00000025434 E003 0.0000000       11 47248639 47248679 41 +      
ENSG00000025434 E004 0.0000000       11 47248680 47248864 185 +      
ENSG00000025434 E005 8.6437921 0.0035720334 0.47657113 0.68467539 11 47248916 47248923 8 + 0.860 0.948 0.332
ENSG00000025434 E006 14.4005444 0.0024460569 0.30011299 0.54053632 11 47248924 47248926 3 + 1.213 1.110 -0.369
ENSG00000025434 E007 28.8674244 0.0013825758 0.87974829 0.94111132 11 47248927 47248955 29 + 1.415 1.422 0.023
ENSG00000025434 E008 42.6400890 0.0009665035 0.34595546 0.58353394 11 47248956 47248999 44 + 1.538 1.592 0.183
ENSG00000025434 E009 2.0334034 0.8595912276 0.48788045 0.69258659 11 47250466 47250551 86 + 0.433 0.436 0.013
ENSG00000025434 E010 0.6536007 0.0706504749 1.00000000   11 47257604 47257622 19 + 0.196 0.190 -0.053
ENSG00000025434 E011 0.4812263 0.0210790933 0.72025691   11 47257623 47257635 13 + 0.196 0.135 -0.635
ENSG00000025434 E012 0.4812263 0.0210790933 0.72025691   11 47257636 47257657 22 + 0.196 0.135 -0.635
ENSG00000025434 E013 0.5227326 0.5121904312 0.75602648   11 47257658 47257696 39 + 0.198 0.138 -0.628
ENSG00000025434 E014 0.3503582 0.0412681862 0.42197657   11 47257697 47257707 11 + 0.196 0.073 -1.633
ENSG00000025434 E015 0.3503582 0.0412681862 0.42197657   11 47257708 47257709 2 + 0.196 0.073 -1.633
ENSG00000025434 E016 0.4812263 0.0210790933 0.72025691   11 47257710 47257735 26 + 0.196 0.135 -0.635
ENSG00000025434 E017 0.6487990 0.2501496723 0.35361072   11 47257917 47257962 46 + 0.328 0.137 -1.607
ENSG00000025434 E018 0.6487990 0.2501496723 0.35361072   11 47257963 47257969 7 + 0.328 0.137 -1.607
ENSG00000025434 E019 0.6487990 0.2501496723 0.35361072   11 47257970 47257970 1 + 0.328 0.137 -1.607
ENSG00000025434 E020 0.8102147 0.3584576464 0.19839924   11 47257971 47257978 8 + 0.430 0.137 -2.186
ENSG00000025434 E021 0.8995767 0.0186821574 0.18517485   11 47257979 47257995 17 + 0.432 0.190 -1.635
ENSG00000025434 E022 1.5891467 0.0117735452 0.08749300 0.25909052 11 47257996 47257997 2 + 0.585 0.282 -1.636
ENSG00000025434 E023 1.5891467 0.0117735452 0.08749300 0.25909052 11 47257998 47258000 3 + 0.585 0.282 -1.636
ENSG00000025434 E024 2.7662200 0.0147502460 0.40339347 0.63103803 11 47258001 47258105 105 + 0.645 0.503 -0.643
ENSG00000025434 E025 1.7532711 0.5974572342 0.88196679 0.94235122 11 47258106 47258129 24 + 0.444 0.393 -0.273
ENSG00000025434 E026 0.7429626 0.0208592695 0.82913392   11 47258130 47258165 36 + 0.196 0.238 0.361
ENSG00000025434 E027 2.7943622 0.2083375941 0.29459270 0.53515867 11 47258166 47258879 714 + 0.698 0.456 -1.101
ENSG00000025434 E028 1.3141662 0.4625566445 0.23349000   11 47258880 47259100 221 + 0.513 0.244 -1.580
ENSG00000025434 E029 0.3751086 0.0373031317 0.72612465   11 47259101 47259179 79 + 0.000 0.136 11.962
ENSG00000025434 E030 4.5578931 0.0068142431 0.97485506 0.98932592 11 47259180 47259184 5 + 0.697 0.686 -0.047
ENSG00000025434 E031 8.4378446 0.0045723226 0.20826816 0.43975636 11 47259185 47259259 75 + 0.786 0.948 0.624
ENSG00000025434 E032 1.3684687 0.1627027449 0.41189965   11 47259361 47259463 103 + 0.195 0.364 1.215
ENSG00000025434 E033 1.0542675 0.1247113118 0.70663915   11 47259464 47259586 123 + 0.329 0.243 -0.596
ENSG00000025434 E034 0.5059767 0.0220979645 0.44080361   11 47259587 47259606 20 + 0.000 0.190 12.570
ENSG00000025434 E035 1.3271501 0.1705100118 0.98640642   11 47259607 47259785 179 + 0.329 0.325 -0.021
ENSG00000025434 E036 1.5224422 0.0125055351 0.74039086 0.86064723 11 47259786 47259790 5 + 0.330 0.391 0.363
ENSG00000025434 E037 60.9611358 0.0010348830 0.95136239 0.97767190 11 47259791 47259979 189 + 1.740 1.732 -0.027
ENSG00000025434 E038 0.6897577 0.0535489995 0.04146318   11 47260397 47260408 12 + 0.433 0.073 -3.222
ENSG00000025434 E039 51.5438840 0.0068815454 0.43256585 0.65316501 11 47260409 47260550 142 + 1.702 1.651 -0.174
ENSG00000025434 E040 51.3758389 0.0152292819 0.41456310 0.63948651 11 47260551 47260675 125 + 1.702 1.646 -0.188
ENSG00000025434 E041 0.1779838 0.0348700666 0.15280720   11 47260676 47260678 3 + 0.196 0.000 -14.074
ENSG00000025434 E042 102.9736381 0.0007779433 0.01525409 0.07902191 11 47261241 47261449 209 + 1.881 1.974 0.314
ENSG00000025434 E043 18.0822615 0.0381360500 0.31725164 0.55720990 11 47261450 47261546 97 + 1.320 1.179 -0.496
ENSG00000025434 E044 67.2112907 0.0010613806 0.93528495 0.96981288 11 47261547 47261726 180 + 1.770 1.769 -0.004
ENSG00000025434 E045 26.6395768 0.0025942464 0.88186114 0.94233495 11 47261727 47261918 192 + 1.395 1.377 -0.062
ENSG00000025434 E046 82.8958774 0.0017962815 0.52178587 0.71615461 11 47261919 47262018 100 + 1.840 1.863 0.078
ENSG00000025434 E047 0.1426347 0.0320986098 0.15303419   11 47262019 47262019 1 + 0.195 0.000 -14.094
ENSG00000025434 E048 63.7883290 0.0010846961 0.40107639 0.62915200 11 47267913 47267944 32 + 1.783 1.736 -0.160
ENSG00000025434 E049 84.2514736 0.0088491437 0.90290374 0.95328552 11 47267945 47268026 82 + 1.866 1.864 -0.006
ENSG00000025434 E050 4.4843771 0.1162011733 0.36205763 0.59738365 11 47268027 47268140 114 + 0.823 0.633 -0.780
ENSG00000025434 E051 6.1935483 0.2431412298 0.24613208 0.48377782 11 47268141 47268260 120 + 0.976 0.728 -0.960
ENSG00000025434 E052 84.0416194 0.0005926019 0.97252248 0.98823432 11 47268261 47268355 95 + 1.874 1.869 -0.019
ENSG00000025434 E053 101.1597885 0.0005676592 0.13272900 0.33608917 11 47268550 47269033 484 + 1.906 1.962 0.188