ENSG00000025293

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000374012 ENSG00000025293 HEK293_OSMI2_6hA HEK293_TMG_6hB PHF20 protein_coding protein_coding 19.90482 11.68795 24.87167 1.754328 1.000274 1.088827 7.874177 4.602470 11.950622 1.0772518 0.3364032 1.3746809 0.37335833 0.3851667 0.48096667 0.09580000 6.003178e-01 5.112448e-14 FALSE TRUE
ENST00000617560 ENSG00000025293 HEK293_OSMI2_6hA HEK293_TMG_6hB PHF20 protein_coding processed_transcript 19.90482 11.68795 24.87167 1.754328 1.000274 1.088827 7.171540 4.591749 7.634191 0.5721921 0.6724127 0.7321814 0.38158333 0.3991000 0.30580000 -0.09330000 4.073750e-01 5.112448e-14   FALSE
MSTRG.20640.2 ENSG00000025293 HEK293_OSMI2_6hA HEK293_TMG_6hB PHF20 protein_coding   19.90482 11.68795 24.87167 1.754328 1.000274 1.088827 1.022802 0.000000 2.221301 0.0000000 0.2009509 7.8017416 0.04044167 0.0000000 0.08903333 0.08903333 5.112448e-14 5.112448e-14 FALSE TRUE
MSTRG.20640.3 ENSG00000025293 HEK293_OSMI2_6hA HEK293_TMG_6hB PHF20 protein_coding   19.90482 11.68795 24.87167 1.754328 1.000274 1.088827 2.380783 1.811174 1.542687 0.5307702 0.2194656 -0.2301017 0.13088750 0.1493667 0.06183333 -0.08753333 5.579981e-02 5.112448e-14   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000025293 E001 0.1308682 0.0326491905 2.044327e-01   20 35771974 35771989 16 + 0.000 0.179 10.523
ENSG00000025293 E002 0.1308682 0.0326491905 2.044327e-01   20 35771990 35771995 6 + 0.000 0.179 12.568
ENSG00000025293 E003 0.2617363 0.0895357590 6.361202e-02   20 35771996 35771997 2 + 0.000 0.303 13.080
ENSG00000025293 E004 0.8912068 0.2080419472 4.730561e-01   20 35771998 35772008 11 + 0.208 0.394 1.265
ENSG00000025293 E005 1.3732410 0.0438279396 8.667375e-01   20 35772009 35772011 3 + 0.347 0.401 0.311
ENSG00000025293 E006 1.5041091 0.1336564586 6.859410e-01 8.264471e-01 20 35772012 35772014 3 + 0.348 0.474 0.689
ENSG00000025293 E007 1.9883561 0.0530931454 9.882906e-01 9.959341e-01 20 35772015 35772021 7 + 0.453 0.479 0.132
ENSG00000025293 E008 9.4542075 0.0035275406 2.599577e-01 4.989862e-01 20 35772022 35772025 4 + 1.016 0.911 -0.391
ENSG00000025293 E009 12.2897432 0.0084133125 7.513750e-02 2.351134e-01 20 35772026 35772032 7 + 1.135 0.961 -0.634
ENSG00000025293 E010 54.5160153 0.0006930369 3.442581e-01 5.818577e-01 20 35772033 35772079 47 + 1.680 1.757 0.262
ENSG00000025293 E011 67.4288386 0.0121577382 3.781902e-04 4.519733e-03 20 35774488 35775058 571 + 1.703 1.965 0.884
ENSG00000025293 E012 1.0095684 0.5258506963 3.886375e-01   20 35782328 35782609 282 + 0.214 0.402 1.260
ENSG00000025293 E013 0.8377416 0.5444233190 4.205806e-01   20 35800094 35800173 80 + 0.149 0.411 1.949
ENSG00000025293 E014 74.4586769 0.0007144698 4.783606e-01 6.859871e-01 20 35801491 35801605 115 + 1.838 1.835 -0.009
ENSG00000025293 E015 0.3337900 0.0382513925 5.291040e-01   20 35801606 35801972 367 + 0.081 0.179 1.328
ENSG00000025293 E016 2.7640749 0.0874713043 2.048858e-01 4.357679e-01 20 35812623 35812719 97 + 0.454 0.706 1.145
ENSG00000025293 E017 9.0175264 0.0069950744 2.164447e-06 5.595567e-05 20 35823258 35823477 220 + 0.723 1.275 2.059
ENSG00000025293 E018 0.0000000       20 35838355 35838442 88 +      
ENSG00000025293 E019 84.9086632 0.0004848433 2.508398e-05 4.625230e-04 20 35842573 35842744 172 + 1.932 1.776 -0.525
ENSG00000025293 E020 0.1779838 0.0352203568 1.000000e+00   20 35845385 35845508 124 + 0.081 0.000 -11.750
ENSG00000025293 E021 47.8140696 0.0009176157 4.863345e-03 3.388896e-02 20 35847350 35847434 85 + 1.682 1.547 -0.459
ENSG00000025293 E022 0.0000000       20 35849389 35849483 95 +      
ENSG00000025293 E023 0.0000000       20 35855231 35855253 23 +      
ENSG00000025293 E024 0.0000000       20 35855254 35855291 38 +      
ENSG00000025293 E025 0.1426347 0.0322058418 1.000000e+00   20 35855301 35855572 272 + 0.080 0.000 -11.785
ENSG00000025293 E026 0.1779838 0.0352203568 1.000000e+00   20 35856356 35856530 175 + 0.081 0.000 -11.750
ENSG00000025293 E027 47.7008359 0.0007338010 2.956259e-03 2.313986e-02 20 35858302 35858381 80 + 1.684 1.541 -0.487
ENSG00000025293 E028 130.5152752 0.0006040317 1.089099e-04 1.604808e-03 20 35863013 35863400 388 + 2.104 1.996 -0.363
ENSG00000025293 E029 67.1065471 0.0008125159 6.571352e-04 7.069792e-03 20 35869438 35869529 92 + 1.830 1.690 -0.472
ENSG00000025293 E030 45.1981374 0.0008168618 4.041865e-04 4.770522e-03 20 35869530 35869551 22 + 1.671 1.486 -0.630
ENSG00000025293 E031 90.2038193 0.0005282425 4.943698e-05 8.235762e-04 20 35870955 35871134 180 + 1.952 1.809 -0.484
ENSG00000025293 E032 78.1453852 0.0017577630 3.860377e-02 1.511366e-01 20 35871650 35871829 180 + 1.871 1.802 -0.233
ENSG00000025293 E033 0.2922838 0.0261113322 5.335754e-01   20 35871830 35871893 64 + 0.081 0.179 1.324
ENSG00000025293 E034 66.4950235 0.0041048756 3.775205e-01 6.102676e-01 20 35899370 35899648 279 + 1.792 1.774 -0.062
ENSG00000025293 E035 45.1913407 0.0008515121 4.536004e-01 6.684059e-01 20 35913249 35913347 99 + 1.629 1.616 -0.043
ENSG00000025293 E036 72.9200965 0.0006257859 3.258578e-01 5.651017e-01 20 35914033 35914197 165 + 1.806 1.878 0.245
ENSG00000025293 E037 0.0000000       20 35917180 35917387 208 +      
ENSG00000025293 E038 99.2457092 0.0006497557 2.613278e-01 5.003887e-01 20 35917484 35917662 179 + 1.966 1.951 -0.049
ENSG00000025293 E039 72.1228279 0.0038068255 4.406140e-01 6.591530e-01 20 35927780 35927879 100 + 1.830 1.818 -0.042
ENSG00000025293 E040 75.7541372 0.0045322393 9.197022e-01 9.620543e-01 20 35931249 35931444 196 + 1.839 1.861 0.073
ENSG00000025293 E041 0.0000000       20 35938632 35938696 65 +      
ENSG00000025293 E042 123.0077896 0.0003735464 9.731113e-02 2.770671e-01 20 35938697 35939108 412 + 2.025 2.111 0.289
ENSG00000025293 E043 74.5276936 0.0056461931 4.833236e-01 6.892913e-01 20 35940864 35941047 184 + 1.840 1.833 -0.023
ENSG00000025293 E044 639.0831775 0.0025834822 1.481286e-06 4.002490e-05 20 35947485 35950370 2886 + 2.720 2.854 0.448