ENSG00000023330

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000310271 ENSG00000023330 HEK293_OSMI2_6hA HEK293_TMG_6hB ALAS1 protein_coding protein_coding 35.16283 40.01584 28.93635 4.775592 0.9876494 -0.4675502 3.605915 5.542896 3.964099 2.3958825 0.6625209 -0.4826126 0.10410000 0.12970000 0.1362333 0.006533333 0.96230094 0.00306531 FALSE TRUE
ENST00000394965 ENSG00000023330 HEK293_OSMI2_6hA HEK293_TMG_6hB ALAS1 protein_coding protein_coding 35.16283 40.01584 28.93635 4.775592 0.9876494 -0.4675502 2.124724 2.673561 3.026388 0.9290894 0.6613917 0.1782076 0.06499583 0.06286667 0.1033333 0.040466667 0.68831321 0.00306531 FALSE TRUE
ENST00000469224 ENSG00000023330 HEK293_OSMI2_6hA HEK293_TMG_6hB ALAS1 protein_coding protein_coding 35.16283 40.01584 28.93635 4.775592 0.9876494 -0.4675502 21.258056 25.055415 13.816825 2.8717073 0.7746190 -0.8582282 0.59463750 0.62790000 0.4768667 -0.151033333 0.07394684 0.00306531 FALSE TRUE
ENST00000484952 ENSG00000023330 HEK293_OSMI2_6hA HEK293_TMG_6hB ALAS1 protein_coding protein_coding 35.16283 40.01584 28.93635 4.775592 0.9876494 -0.4675502 5.436729 4.165583 6.025365 0.6448918 0.2877599 0.5314634 0.15831250 0.10376667 0.2094000 0.105633333 0.00306531 0.00306531 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000023330 E001 0.000000       3 52198086 52198093 8 +      
ENSG00000023330 E002 1.773407 0.0982357952 9.082666e-01 9.562944e-01 3 52198094 52198124 31 + 0.430 0.465 0.179
ENSG00000023330 E003 2.380700 0.1041917973 8.817367e-01 9.422713e-01 3 52198125 52198131 7 + 0.548 0.530 -0.085
ENSG00000023330 E004 43.516906 0.0009827801 1.319905e-01 3.349475e-01 3 52198132 52198159 28 + 1.577 1.661 0.287
ENSG00000023330 E005 134.025989 0.0003827631 7.445687e-01 8.634224e-01 3 52198160 52198255 96 + 2.119 2.108 -0.036
ENSG00000023330 E006 69.365220 0.0006611515 3.096478e-07 1.013791e-05 3 52198672 52198848 177 + 1.957 1.736 -0.745
ENSG00000023330 E007 84.350563 0.0166850236 5.652889e-01 7.468942e-01 3 52199210 52199220 11 + 1.888 1.929 0.138
ENSG00000023330 E008 308.312888 0.0001956381 7.021586e-03 4.474398e-02 3 52199221 52199440 220 + 2.507 2.451 -0.187
ENSG00000023330 E009 107.205266 0.0005317197 2.228735e-02 1.035570e-01 3 52202507 52202509 3 + 2.067 1.986 -0.273
ENSG00000023330 E010 348.009908 0.0015022340 2.907320e-01 5.313607e-01 3 52202510 52202734 225 + 2.541 2.514 -0.091
ENSG00000023330 E011 300.391085 0.0048892306 8.368882e-01 9.177103e-01 3 52203863 52204012 150 + 2.453 2.463 0.034
ENSG00000023330 E012 336.211244 0.0037526077 7.600320e-01 8.725548e-01 3 52204693 52204915 223 + 2.501 2.513 0.041
ENSG00000023330 E013 335.489065 0.0003400206 7.401151e-01 8.604991e-01 3 52205839 52206023 185 + 2.515 2.507 -0.024
ENSG00000023330 E014 254.464922 0.0002432000 7.251961e-01 8.512515e-01 3 52206572 52206631 60 + 2.396 2.388 -0.029
ENSG00000023330 E015 338.155779 0.0006323906 8.764248e-01 9.394205e-01 3 52206632 52206751 120 + 2.512 2.516 0.013
ENSG00000023330 E016 349.515866 0.0001912511 6.468347e-02 2.131759e-01 3 52208083 52208247 165 + 2.503 2.540 0.121
ENSG00000023330 E017 308.148218 0.0004677668 8.085551e-01 9.013118e-01 3 52211283 52211451 169 + 2.469 2.475 0.018
ENSG00000023330 E018 213.516493 0.0013400264 7.769632e-01 8.829018e-01 3 52211452 52211545 94 + 2.308 2.317 0.032
ENSG00000023330 E019 117.861003 0.0003408911 7.759815e-01 8.823772e-01 3 52211546 52211551 6 + 2.063 2.053 -0.034
ENSG00000023330 E020 283.447512 0.0001924556 1.910432e-02 9.281781e-02 3 52212258 52212420 163 + 2.401 2.452 0.170
ENSG00000023330 E021 7.242960 0.1711255013 6.699678e-01 8.162651e-01 3 52212421 52212507 87 + 0.941 0.873 -0.259
ENSG00000023330 E022 256.727506 0.0002037341 2.868031e-03 2.261178e-02 3 52214020 52214327 308 + 2.347 2.416 0.229