ENSG00000013374

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000413040 ENSG00000013374 HEK293_OSMI2_6hA HEK293_TMG_6hB NUB1 protein_coding protein_coding 59.41386 38.31304 81.61039 8.920237 3.701107 1.090717 19.588002 17.76266 24.18654 4.909731 1.242753 0.4451411 0.3493042 0.4508667 0.2962000 -0.15466667 2.924236e-02 6.612256e-22 FALSE TRUE
ENST00000468404 ENSG00000013374 HEK293_OSMI2_6hA HEK293_TMG_6hB NUB1 protein_coding nonsense_mediated_decay 59.41386 38.31304 81.61039 8.920237 3.701107 1.090717 21.557074 13.22186 25.58608 3.680090 1.473893 0.9519073 0.3699292 0.3343000 0.3131333 -0.02116667 9.059937e-01 6.612256e-22 TRUE TRUE
ENST00000483358 ENSG00000013374 HEK293_OSMI2_6hA HEK293_TMG_6hB NUB1 protein_coding protein_coding 59.41386 38.31304 81.61039 8.920237 3.701107 1.090717 8.229373 0.00000 18.95184 0.000000 2.587007 10.8888834 0.1017250 0.0000000 0.2349333 0.23493333 6.612256e-22 6.612256e-22 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_6hB HEK293_OSMI2_6hA log2fold_HEK293_OSMI2_6hA_HEK293_TMG_6hB
ENSG00000013374 E001 0.5352391 0.1842609052 2.934402e-01   7 151341697 151341707 11 + 0.103 0.328 2.081
ENSG00000013374 E002 0.5352391 0.1842609052 2.934402e-01   7 151341708 151341711 4 + 0.103 0.328 2.081
ENSG00000013374 E003 0.5352391 0.1842609052 2.934402e-01   7 151341712 151341717 6 + 0.103 0.328 2.081
ENSG00000013374 E004 4.6375202 0.0697734554 6.920341e-01 8.303539e-01 7 151341718 151341771 54 + 0.781 0.743 -0.152
ENSG00000013374 E005 14.7107875 0.0046001567 3.476763e-01 5.851098e-01 7 151341772 151341803 32 + 1.218 1.164 -0.194
ENSG00000013374 E006 19.6054441 0.0029491980 7.140464e-01 8.443589e-01 7 151341804 151341811 8 + 1.307 1.310 0.010
ENSG00000013374 E007 96.1907742 0.0005403429 5.209084e-01 7.155055e-01 7 151341812 151341846 35 + 1.976 1.986 0.036
ENSG00000013374 E008 15.1202405 0.0030827474 9.640081e-01 9.839063e-01 7 151341847 151341965 119 + 1.182 1.218 0.127
ENSG00000013374 E009 0.7035494 0.0369122261 4.783395e-01   7 151345345 151345347 3 + 0.257 0.143 -1.050
ENSG00000013374 E010 268.6555087 0.0013140080 1.962911e-04 2.633700e-03 7 151345348 151345466 119 + 2.452 2.383 -0.229
ENSG00000013374 E011 402.3211422 0.0019428146 1.648460e-06 4.405136e-05 7 151349073 151349240 168 + 2.635 2.544 -0.303
ENSG00000013374 E012 0.8096672 0.0461975067 2.949195e-01   7 151351120 151351327 208 + 0.317 0.142 -1.477
ENSG00000013374 E013 0.9217543 0.0176059664 6.308404e-01   7 151351328 151351423 96 + 0.317 0.249 -0.471
ENSG00000013374 E014 275.4419126 0.0031153916 1.918795e-02 9.310957e-02 7 151351424 151351482 59 + 2.450 2.412 -0.129
ENSG00000013374 E015 2.8919447 0.2154008839 1.166858e-01 3.103173e-01 7 151352093 151352193 101 + 0.700 0.339 -1.762
ENSG00000013374 E016 0.7499275 0.0173969323 9.062723e-01   7 151352252 151352366 115 + 0.256 0.249 -0.051
ENSG00000013374 E017 250.6922902 0.0031306396 7.072880e-04 7.505703e-03 7 151352812 151352882 71 + 2.426 2.346 -0.268
ENSG00000013374 E018 530.0408568 0.0020327885 3.706321e-06 8.892605e-05 7 151355768 151355950 183 + 2.751 2.671 -0.268
ENSG00000013374 E019 199.3852994 0.0016303292 3.597532e-03 2.691975e-02 7 151356128 151356130 3 + 2.320 2.261 -0.196
ENSG00000013374 E020 388.0525227 0.0015431212 1.336616e-02 7.190450e-02 7 151356131 151356222 92 + 2.596 2.569 -0.090
ENSG00000013374 E021 362.8571910 0.0008754195 6.354266e-05 1.022941e-03 7 151360141 151360247 107 + 2.577 2.521 -0.185
ENSG00000013374 E022 380.4141203 0.0002575157 5.665481e-08 2.231800e-06 7 151360248 151360914 667 + 2.604 2.535 -0.228
ENSG00000013374 E023 51.4391738 0.0008100803 6.126844e-02 2.056415e-01 7 151361059 151361249 191 + 1.658 1.786 0.434
ENSG00000013374 E024 268.9315022 0.0002546289 9.996578e-01 1.000000e+00 7 151366939 151367125 187 + 2.411 2.446 0.117
ENSG00000013374 E025 209.6302554 0.0002697204 1.146571e-01 3.068855e-01 7 151367861 151367968 108 + 2.287 2.361 0.250
ENSG00000013374 E026 1.1326708 0.0142100815 4.561906e-01   7 151368733 151368734 2 + 0.256 0.407 0.949
ENSG00000013374 E027 178.1564766 0.0004031490 2.197941e-02 1.025116e-01 7 151368735 151368829 95 + 2.206 2.305 0.330
ENSG00000013374 E028 133.3929255 0.0004888754 4.920344e-04 5.601204e-03 7 151368830 151368887 58 + 2.060 2.206 0.487
ENSG00000013374 E029 8.7166617 0.2504327912 2.742871e-01 5.142602e-01 7 151368888 151369193 306 + 0.829 1.124 1.099
ENSG00000013374 E030 166.3793875 0.0025817993 3.959174e-05 6.819054e-04 7 151374097 151374201 105 + 2.136 2.321 0.621
ENSG00000013374 E031 12.4555131 0.0340773725 2.259298e-03 1.879498e-02 7 151374202 151374243 42 + 0.915 1.321 1.463
ENSG00000013374 E032 21.2473775 0.2271500587 5.217341e-01 7.161183e-01 7 151374244 151374624 381 + 1.233 1.461 0.792
ENSG00000013374 E033 147.2882539 0.0747118566 4.602536e-02 1.701163e-01 7 151375848 151375943 96 + 2.043 2.295 0.843
ENSG00000013374 E034 211.8961860 1.4612563208 4.365218e-01 6.561430e-01 7 151376634 151376811 178 + 2.194 2.456 0.874
ENSG00000013374 E035 765.8036536 0.0069684653 1.751142e-07 6.131633e-06 7 151377047 151378429 1383 + 2.774 2.994 0.733
ENSG00000013374 E036 11.1158973 0.0213092383 4.081580e-01 6.345992e-01 7 151378430 151378449 20 + 1.018 1.150 0.475
ENSG00000013374 E037 3.4488273 0.1360047874 5.979143e-01 7.688881e-01 7 151378541 151378700 160 + 0.595 0.696 0.435
ENSG00000013374 E038 3.0667768 0.0077371492 1.444472e-01 3.540720e-01 7 151379492 151379530 39 + 0.677 0.469 -0.950